Current trends in separation of plasmid DNA vaccines: A review

Ashraf GHANEM, Frady Gouany

Research output: Contribution to journalReview article

44 Citations (Scopus)

Abstract

Plasmid DNA (pDNA)-based vaccines offer more rapid avenues for development and production if compared to those of conventional virus-based vaccines. They do not rely on time- or labour-intensive cell culture processes and allow greater flexibility in shipping and storage. Stimulating antibodies and cell-mediated components of the immune system are considered as some of the major advantages associated with the use of pDNA vaccines. This review summarizes the current trends in the purification of pDNA vaccines for practical and analytical applications. Special attention is paid to chromatographic techniques aimed at reducing the steps of final purification, post primary isolation and intermediate recovery, in order to reduce the number of steps necessary to reach a purified end product from the crude plasmid
Original languageEnglish
Pages (from-to)1-15
Number of pages15
JournalAnalytica Chimica Acta
Volume760
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

DNA Vaccines
vaccine
plasmid
Plasmids
DNA
Purification
purification
Vaccines
Immune system
immune system
Cellular Structures
Freight transportation
shipping
Viruses
Cell culture
antibody
Immune System
virus
Cell Culture Techniques
labor

Cite this

@article{46d69d513a2b4e3591bee6273c38a074,
title = "Current trends in separation of plasmid DNA vaccines: A review",
abstract = "Plasmid DNA (pDNA)-based vaccines offer more rapid avenues for development and production if compared to those of conventional virus-based vaccines. They do not rely on time- or labour-intensive cell culture processes and allow greater flexibility in shipping and storage. Stimulating antibodies and cell-mediated components of the immune system are considered as some of the major advantages associated with the use of pDNA vaccines. This review summarizes the current trends in the purification of pDNA vaccines for practical and analytical applications. Special attention is paid to chromatographic techniques aimed at reducing the steps of final purification, post primary isolation and intermediate recovery, in order to reduce the number of steps necessary to reach a purified end product from the crude plasmid",
keywords = "Analysis, Plasmid DNA, Chromatography",
author = "Ashraf GHANEM and Frady Gouany",
year = "2013",
doi = "10.1016/j.aca.2012.11.006",
language = "English",
volume = "760",
pages = "1--15",
journal = "Analytica Chimica Acta",
issn = "0003-2670",
publisher = "Elsevier",
number = "1",

}

Current trends in separation of plasmid DNA vaccines: A review. / GHANEM, Ashraf; Gouany, Frady.

In: Analytica Chimica Acta, Vol. 760, No. 1, 2013, p. 1-15.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Current trends in separation of plasmid DNA vaccines: A review

AU - GHANEM, Ashraf

AU - Gouany, Frady

PY - 2013

Y1 - 2013

N2 - Plasmid DNA (pDNA)-based vaccines offer more rapid avenues for development and production if compared to those of conventional virus-based vaccines. They do not rely on time- or labour-intensive cell culture processes and allow greater flexibility in shipping and storage. Stimulating antibodies and cell-mediated components of the immune system are considered as some of the major advantages associated with the use of pDNA vaccines. This review summarizes the current trends in the purification of pDNA vaccines for practical and analytical applications. Special attention is paid to chromatographic techniques aimed at reducing the steps of final purification, post primary isolation and intermediate recovery, in order to reduce the number of steps necessary to reach a purified end product from the crude plasmid

AB - Plasmid DNA (pDNA)-based vaccines offer more rapid avenues for development and production if compared to those of conventional virus-based vaccines. They do not rely on time- or labour-intensive cell culture processes and allow greater flexibility in shipping and storage. Stimulating antibodies and cell-mediated components of the immune system are considered as some of the major advantages associated with the use of pDNA vaccines. This review summarizes the current trends in the purification of pDNA vaccines for practical and analytical applications. Special attention is paid to chromatographic techniques aimed at reducing the steps of final purification, post primary isolation and intermediate recovery, in order to reduce the number of steps necessary to reach a purified end product from the crude plasmid

KW - Analysis

KW - Plasmid DNA

KW - Chromatography

U2 - 10.1016/j.aca.2012.11.006

DO - 10.1016/j.aca.2012.11.006

M3 - Review article

VL - 760

SP - 1

EP - 15

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

IS - 1

ER -