Current trends in separation of plasmid DNA vaccines: A review

Ashraf GHANEM, Frady Gouany

    Research output: Contribution to journalReview article

    43 Citations (Scopus)

    Abstract

    Plasmid DNA (pDNA)-based vaccines offer more rapid avenues for development and production if compared to those of conventional virus-based vaccines. They do not rely on time- or labour-intensive cell culture processes and allow greater flexibility in shipping and storage. Stimulating antibodies and cell-mediated components of the immune system are considered as some of the major advantages associated with the use of pDNA vaccines. This review summarizes the current trends in the purification of pDNA vaccines for practical and analytical applications. Special attention is paid to chromatographic techniques aimed at reducing the steps of final purification, post primary isolation and intermediate recovery, in order to reduce the number of steps necessary to reach a purified end product from the crude plasmid
    Original languageEnglish
    Pages (from-to)1-15
    Number of pages15
    JournalAnalytica Chimica Acta
    Volume760
    Issue number1
    DOIs
    Publication statusPublished - 2013

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    DNA Vaccines
    vaccine
    plasmid
    Plasmids
    DNA
    Purification
    purification
    Vaccines
    Immune system
    immune system
    Cellular Structures
    Freight transportation
    shipping
    Viruses
    Cell culture
    antibody
    Immune System
    virus
    Cell Culture Techniques
    labor

    Cite this

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    abstract = "Plasmid DNA (pDNA)-based vaccines offer more rapid avenues for development and production if compared to those of conventional virus-based vaccines. They do not rely on time- or labour-intensive cell culture processes and allow greater flexibility in shipping and storage. Stimulating antibodies and cell-mediated components of the immune system are considered as some of the major advantages associated with the use of pDNA vaccines. This review summarizes the current trends in the purification of pDNA vaccines for practical and analytical applications. Special attention is paid to chromatographic techniques aimed at reducing the steps of final purification, post primary isolation and intermediate recovery, in order to reduce the number of steps necessary to reach a purified end product from the crude plasmid",
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    Current trends in separation of plasmid DNA vaccines: A review. / GHANEM, Ashraf; Gouany, Frady.

    In: Analytica Chimica Acta, Vol. 760, No. 1, 2013, p. 1-15.

    Research output: Contribution to journalReview article

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    AU - GHANEM, Ashraf

    AU - Gouany, Frady

    PY - 2013

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    KW - Analysis

    KW - Plasmid DNA

    KW - Chromatography

    U2 - 10.1016/j.aca.2012.11.006

    DO - 10.1016/j.aca.2012.11.006

    M3 - Review article

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    JO - Analytica Chimica Acta

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