Deciphering the Evolutionary History of Arowana Fishes (Teleostei, Osteoglossiformes, Osteoglossidae)

Insight from Comparative Cytogenomics

Marcelo de Bello Cioffi, Petr Ráb, Tariq Ezaz, Luiz Antonio Carlos Bertollo, Sebastien Lavoué, Ezequiel Aguiar de Oliveira, Alexandr Sember, Wagner Franco Molina, Fernando Henrique Santos de Souza, Zuzana Majtánová, Thomas Liehr, Ahmed Basheer Hamid Al-Rikabi, Cassia Fernanda Yano, Patrik Viana, Eliana Feldberg, Peter Unmack, Terumi Hatanaka, Alongklod Tanomtong, Manolo Fernandez Perez

Research output: Contribution to journalArticle

Abstract

Arowanas (Osteoglossinae) are charismatic freshwater fishes with six species and two genera (Osteoglossum and Scleropages) distributed in South America, Asia, and Australia. In an attempt to provide a better assessment of the processes shaping their evolution, we employed a set of cytogenetic and genomic approaches, including i) molecular cytogenetic analyses using C- and CMA3/DAPI staining, repetitive DNA mapping, comparative genomic hybridization (CGH), and Zoo-FISH, along with ii) the genotypic analyses of single nucleotide polymorphisms (SNPs) generated by diversity array technology sequencing (DArTseq). We observed diploid chromosome numbers of 2n = 56 and 54 in O. bicirrhosum and O. ferreirai, respectively, and 2n = 50 in S. formosus, while S. jardinii and S. leichardti presented 2n = 48 and 44, respectively. A time-calibrated phylogenetic tree revealed that Osteoglossum and Scleropages divergence occurred approximately 50 million years ago (MYA), at the time of the final separation of Australia and South America (with Antarctica). Asian S. formosus and Australian Scleropages diverged about 35.5 MYA, substantially after the latest terrestrial connection between Australia and Southeast Asia through the Indian plate movement. Our combined data provided a comprehensive perspective of the cytogenomic diversity and evolution of arowana species on a timescale.

Original languageEnglish
Article number4296
Pages (from-to)1-19
Number of pages19
JournalInternational Journal of Molecular Sciences
Volume20
Issue number17
DOIs
Publication statusPublished - 2 Sep 2019

Fingerprint

South America
fishes
Chromosomes
Nucleotides
Polymorphism
Fish
Fishes
DNA
History
histories
Southeastern Asia
South Australia
Process Assessment (Health Care)
Comparative Genomic Hybridization
Cytogenetic Analysis
Fresh Water
Diploidy
Cytogenetics
Southeast Asia
Single Nucleotide Polymorphism

Cite this

Cioffi, Marcelo de Bello ; Ráb, Petr ; Ezaz, Tariq ; Bertollo, Luiz Antonio Carlos ; Lavoué, Sebastien ; Oliveira, Ezequiel Aguiar de ; Sember, Alexandr ; Molina, Wagner Franco ; Souza, Fernando Henrique Santos de ; Majtánová, Zuzana ; Liehr, Thomas ; Al-Rikabi, Ahmed Basheer Hamid ; Yano, Cassia Fernanda ; Viana, Patrik ; Feldberg, Eliana ; Unmack, Peter ; Hatanaka, Terumi ; Tanomtong, Alongklod ; Perez, Manolo Fernandez. / Deciphering the Evolutionary History of Arowana Fishes (Teleostei, Osteoglossiformes, Osteoglossidae) : Insight from Comparative Cytogenomics. In: International Journal of Molecular Sciences. 2019 ; Vol. 20, No. 17. pp. 1-19.
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abstract = "Arowanas (Osteoglossinae) are charismatic freshwater fishes with six species and two genera (Osteoglossum and Scleropages) distributed in South America, Asia, and Australia. In an attempt to provide a better assessment of the processes shaping their evolution, we employed a set of cytogenetic and genomic approaches, including i) molecular cytogenetic analyses using C- and CMA3/DAPI staining, repetitive DNA mapping, comparative genomic hybridization (CGH), and Zoo-FISH, along with ii) the genotypic analyses of single nucleotide polymorphisms (SNPs) generated by diversity array technology sequencing (DArTseq). We observed diploid chromosome numbers of 2n = 56 and 54 in O. bicirrhosum and O. ferreirai, respectively, and 2n = 50 in S. formosus, while S. jardinii and S. leichardti presented 2n = 48 and 44, respectively. A time-calibrated phylogenetic tree revealed that Osteoglossum and Scleropages divergence occurred approximately 50 million years ago (MYA), at the time of the final separation of Australia and South America (with Antarctica). Asian S. formosus and Australian Scleropages diverged about 35.5 MYA, substantially after the latest terrestrial connection between Australia and Southeast Asia through the Indian plate movement. Our combined data provided a comprehensive perspective of the cytogenomic diversity and evolution of arowana species on a timescale.",
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Cioffi, MDB, Ráb, P, Ezaz, T, Bertollo, LAC, Lavoué, S, Oliveira, EAD, Sember, A, Molina, WF, Souza, FHSD, Majtánová, Z, Liehr, T, Al-Rikabi, ABH, Yano, CF, Viana, P, Feldberg, E, Unmack, P, Hatanaka, T, Tanomtong, A & Perez, MF 2019, 'Deciphering the Evolutionary History of Arowana Fishes (Teleostei, Osteoglossiformes, Osteoglossidae): Insight from Comparative Cytogenomics', International Journal of Molecular Sciences, vol. 20, no. 17, 4296, pp. 1-19. https://doi.org/10.3390/ijms20174296

Deciphering the Evolutionary History of Arowana Fishes (Teleostei, Osteoglossiformes, Osteoglossidae) : Insight from Comparative Cytogenomics. / Cioffi, Marcelo de Bello; Ráb, Petr; Ezaz, Tariq; Bertollo, Luiz Antonio Carlos; Lavoué, Sebastien; Oliveira, Ezequiel Aguiar de; Sember, Alexandr; Molina, Wagner Franco; Souza, Fernando Henrique Santos de; Majtánová, Zuzana; Liehr, Thomas; Al-Rikabi, Ahmed Basheer Hamid; Yano, Cassia Fernanda; Viana, Patrik; Feldberg, Eliana; Unmack, Peter; Hatanaka, Terumi; Tanomtong, Alongklod; Perez, Manolo Fernandez.

In: International Journal of Molecular Sciences, Vol. 20, No. 17, 4296, 02.09.2019, p. 1-19.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Deciphering the Evolutionary History of Arowana Fishes (Teleostei, Osteoglossiformes, Osteoglossidae)

T2 - Insight from Comparative Cytogenomics

AU - Cioffi, Marcelo de Bello

AU - Ráb, Petr

AU - Ezaz, Tariq

AU - Bertollo, Luiz Antonio Carlos

AU - Lavoué, Sebastien

AU - Oliveira, Ezequiel Aguiar de

AU - Sember, Alexandr

AU - Molina, Wagner Franco

AU - Souza, Fernando Henrique Santos de

AU - Majtánová, Zuzana

AU - Liehr, Thomas

AU - Al-Rikabi, Ahmed Basheer Hamid

AU - Yano, Cassia Fernanda

AU - Viana, Patrik

AU - Feldberg, Eliana

AU - Unmack, Peter

AU - Hatanaka, Terumi

AU - Tanomtong, Alongklod

AU - Perez, Manolo Fernandez

PY - 2019/9/2

Y1 - 2019/9/2

N2 - Arowanas (Osteoglossinae) are charismatic freshwater fishes with six species and two genera (Osteoglossum and Scleropages) distributed in South America, Asia, and Australia. In an attempt to provide a better assessment of the processes shaping their evolution, we employed a set of cytogenetic and genomic approaches, including i) molecular cytogenetic analyses using C- and CMA3/DAPI staining, repetitive DNA mapping, comparative genomic hybridization (CGH), and Zoo-FISH, along with ii) the genotypic analyses of single nucleotide polymorphisms (SNPs) generated by diversity array technology sequencing (DArTseq). We observed diploid chromosome numbers of 2n = 56 and 54 in O. bicirrhosum and O. ferreirai, respectively, and 2n = 50 in S. formosus, while S. jardinii and S. leichardti presented 2n = 48 and 44, respectively. A time-calibrated phylogenetic tree revealed that Osteoglossum and Scleropages divergence occurred approximately 50 million years ago (MYA), at the time of the final separation of Australia and South America (with Antarctica). Asian S. formosus and Australian Scleropages diverged about 35.5 MYA, substantially after the latest terrestrial connection between Australia and Southeast Asia through the Indian plate movement. Our combined data provided a comprehensive perspective of the cytogenomic diversity and evolution of arowana species on a timescale.

AB - Arowanas (Osteoglossinae) are charismatic freshwater fishes with six species and two genera (Osteoglossum and Scleropages) distributed in South America, Asia, and Australia. In an attempt to provide a better assessment of the processes shaping their evolution, we employed a set of cytogenetic and genomic approaches, including i) molecular cytogenetic analyses using C- and CMA3/DAPI staining, repetitive DNA mapping, comparative genomic hybridization (CGH), and Zoo-FISH, along with ii) the genotypic analyses of single nucleotide polymorphisms (SNPs) generated by diversity array technology sequencing (DArTseq). We observed diploid chromosome numbers of 2n = 56 and 54 in O. bicirrhosum and O. ferreirai, respectively, and 2n = 50 in S. formosus, while S. jardinii and S. leichardti presented 2n = 48 and 44, respectively. A time-calibrated phylogenetic tree revealed that Osteoglossum and Scleropages divergence occurred approximately 50 million years ago (MYA), at the time of the final separation of Australia and South America (with Antarctica). Asian S. formosus and Australian Scleropages diverged about 35.5 MYA, substantially after the latest terrestrial connection between Australia and Southeast Asia through the Indian plate movement. Our combined data provided a comprehensive perspective of the cytogenomic diversity and evolution of arowana species on a timescale.

KW - biogeography

KW - DArTseq

KW - evolution

KW - genetic diversity

KW - Gondwana

UR - http://www.scopus.com/inward/record.url?scp=85071778993&partnerID=8YFLogxK

U2 - 10.3390/ijms20174296

DO - 10.3390/ijms20174296

M3 - Article

VL - 20

SP - 1

EP - 19

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

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