Determination of triasulfuron in soil

affinity chromatography as a soil extract cleanup procedure

R Ghildyal, M Kariofillis

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Triasulfuron forms one of the group of sulfonylurea herbicides. These are used widely for controlling weeds as they are effective at very low application rates. This effectiveness is responsible for the crop losses due to persistence of trace amounts of the herbicides (< or = 100 pg/g) in the soil. The numerous immunoassays described have been constrained by the fact that the soil extract contains co extractants which interfere in the assays, so much so, that these assays are useless at low levels of herbicide. We describe here the preparation and application of an immunoaffinity column which binds specifically to triasulfuron, thus cleaning up the soil extract. The experiment design is such that this also leads to concentration of the triasulfuron, making it easier to assay reliably using ELISA. Six different soil types were used to validate this procedure. In most cases, the herbicide content could be detected at 100 pg/g (critical phytotoxic herbicide level in soil) with a variation of +/- 20% in the readings.

Original languageEnglish
Pages (from-to)207-15
Number of pages9
JournalJournal of Biochemical and Biophysical Methods
Volume30
Issue number2-3
DOIs
Publication statusPublished - Jun 1995
Externally publishedYes

Fingerprint

Affinity chromatography
Herbicides
Affinity Chromatography
Soil
Soils
Assays
Immunoassay
Crops
Reading
Cleaning
Enzyme-Linked Immunosorbent Assay
triasulfuron
Experiments

Cite this

@article{a8a5c51faf12420babd3252238970c3c,
title = "Determination of triasulfuron in soil: affinity chromatography as a soil extract cleanup procedure",
abstract = "Triasulfuron forms one of the group of sulfonylurea herbicides. These are used widely for controlling weeds as they are effective at very low application rates. This effectiveness is responsible for the crop losses due to persistence of trace amounts of the herbicides (< or = 100 pg/g) in the soil. The numerous immunoassays described have been constrained by the fact that the soil extract contains co extractants which interfere in the assays, so much so, that these assays are useless at low levels of herbicide. We describe here the preparation and application of an immunoaffinity column which binds specifically to triasulfuron, thus cleaning up the soil extract. The experiment design is such that this also leads to concentration of the triasulfuron, making it easier to assay reliably using ELISA. Six different soil types were used to validate this procedure. In most cases, the herbicide content could be detected at 100 pg/g (critical phytotoxic herbicide level in soil) with a variation of +/- 20{\%} in the readings.",
keywords = "Buffers, Chromatography, Affinity, Enzyme-Linked Immunosorbent Assay, Filtration, Herbicides, Hydrogen-Ion Concentration, Sensitivity and Specificity, Soil, Soil Pollutants, Sulfonylurea Compounds, Journal Article, Research Support, Non-U.S. Gov't",
author = "R Ghildyal and M Kariofillis",
year = "1995",
month = "6",
doi = "10.1016/0165-022X(94)00071-K",
language = "English",
volume = "30",
pages = "207--15",
journal = "Journal of Biochemical and Biophysical Methods",
issn = "0165-022X",
publisher = "Elsevier",
number = "2-3",

}

Determination of triasulfuron in soil : affinity chromatography as a soil extract cleanup procedure. / Ghildyal, R; Kariofillis, M.

In: Journal of Biochemical and Biophysical Methods, Vol. 30, No. 2-3, 06.1995, p. 207-15.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Determination of triasulfuron in soil

T2 - affinity chromatography as a soil extract cleanup procedure

AU - Ghildyal, R

AU - Kariofillis, M

PY - 1995/6

Y1 - 1995/6

N2 - Triasulfuron forms one of the group of sulfonylurea herbicides. These are used widely for controlling weeds as they are effective at very low application rates. This effectiveness is responsible for the crop losses due to persistence of trace amounts of the herbicides (< or = 100 pg/g) in the soil. The numerous immunoassays described have been constrained by the fact that the soil extract contains co extractants which interfere in the assays, so much so, that these assays are useless at low levels of herbicide. We describe here the preparation and application of an immunoaffinity column which binds specifically to triasulfuron, thus cleaning up the soil extract. The experiment design is such that this also leads to concentration of the triasulfuron, making it easier to assay reliably using ELISA. Six different soil types were used to validate this procedure. In most cases, the herbicide content could be detected at 100 pg/g (critical phytotoxic herbicide level in soil) with a variation of +/- 20% in the readings.

AB - Triasulfuron forms one of the group of sulfonylurea herbicides. These are used widely for controlling weeds as they are effective at very low application rates. This effectiveness is responsible for the crop losses due to persistence of trace amounts of the herbicides (< or = 100 pg/g) in the soil. The numerous immunoassays described have been constrained by the fact that the soil extract contains co extractants which interfere in the assays, so much so, that these assays are useless at low levels of herbicide. We describe here the preparation and application of an immunoaffinity column which binds specifically to triasulfuron, thus cleaning up the soil extract. The experiment design is such that this also leads to concentration of the triasulfuron, making it easier to assay reliably using ELISA. Six different soil types were used to validate this procedure. In most cases, the herbicide content could be detected at 100 pg/g (critical phytotoxic herbicide level in soil) with a variation of +/- 20% in the readings.

KW - Buffers

KW - Chromatography, Affinity

KW - Enzyme-Linked Immunosorbent Assay

KW - Filtration

KW - Herbicides

KW - Hydrogen-Ion Concentration

KW - Sensitivity and Specificity

KW - Soil

KW - Soil Pollutants

KW - Sulfonylurea Compounds

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/0165-022X(94)00071-K

DO - 10.1016/0165-022X(94)00071-K

M3 - Article

VL - 30

SP - 207

EP - 215

JO - Journal of Biochemical and Biophysical Methods

JF - Journal of Biochemical and Biophysical Methods

SN - 0165-022X

IS - 2-3

ER -