Determining the relative sensitivity of benthic diatoms to atrazine using rapid toxicity testing: A novel method

Rebecca Wood, Simon Mitrovic, Ben KEFFORD

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Herbicides pose a potential threat to aquatic ecosystems, especially to phototrophic organisms such as benthic diatoms. Benthic diatoms may be a valuable indicator of the toxic impacts of herbicides in aquatic systems. However, this requires information on the herbicide sensitivity of awide range of freshwater benthic diatomtaxa. Unfortunately this information is only available for a limited number of species as current methods of developing new algae toxicity tests on individual taxa are lengthy and costly. To address this issue, we developed a new rapid toxicity test method to test natural benthic communities, from which the relative herbicide sensitivity of many individual taxa can be derived. This involved the collection of natural benthic communities from rocks in situ, which were placed directly into laboratory toxicity tests. Sensitivity data for several diatom genera in a 48 hour exposure toxicity testwere produced,without the need for cultures or multiple site visits. After exposure to the highest treatment of atrazine (500 µg L-1) therewere significant declines of healthy cells in the most sensitive genera: Gomphonema declined by 74%, Amphora by 62%, Cymbella by 54% and Ulnaria by 34% compared to control levels. In contrast, the genera, Eunotia, Achnanthidium and Navicula, had no statistically significant decline in cell health. This method can identify the diatom taxa most at risk of herbicide toxicity within the natural benthic diatom community. The rapid toxicity testing method presented is a simple and effective method to obtain sensitivity data for multiple taxa within a natural benthic diatom community in a relatively short period of time.
    Original languageEnglish
    Pages (from-to)421-427
    Number of pages7
    JournalScience of the Total Environment
    Volume485-486
    DOIs
    Publication statusPublished - 2014

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    Atrazine
    Herbicides
    atrazine
    Toxicity
    diatom
    herbicide
    toxicity
    Testing
    toxicity test
    benthos
    Aquatic ecosystems
    testing method
    Poisons
    Level control
    Algae
    aquatic ecosystem
    method
    alga
    Rocks
    Cells

    Cite this

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    abstract = "Herbicides pose a potential threat to aquatic ecosystems, especially to phototrophic organisms such as benthic diatoms. Benthic diatoms may be a valuable indicator of the toxic impacts of herbicides in aquatic systems. However, this requires information on the herbicide sensitivity of awide range of freshwater benthic diatomtaxa. Unfortunately this information is only available for a limited number of species as current methods of developing new algae toxicity tests on individual taxa are lengthy and costly. To address this issue, we developed a new rapid toxicity test method to test natural benthic communities, from which the relative herbicide sensitivity of many individual taxa can be derived. This involved the collection of natural benthic communities from rocks in situ, which were placed directly into laboratory toxicity tests. Sensitivity data for several diatom genera in a 48 hour exposure toxicity testwere produced,without the need for cultures or multiple site visits. After exposure to the highest treatment of atrazine (500 µg L-1) therewere significant declines of healthy cells in the most sensitive genera: Gomphonema declined by 74{\%}, Amphora by 62{\%}, Cymbella by 54{\%} and Ulnaria by 34{\%} compared to control levels. In contrast, the genera, Eunotia, Achnanthidium and Navicula, had no statistically significant decline in cell health. This method can identify the diatom taxa most at risk of herbicide toxicity within the natural benthic diatom community. The rapid toxicity testing method presented is a simple and effective method to obtain sensitivity data for multiple taxa within a natural benthic diatom community in a relatively short period of time.",
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    Determining the relative sensitivity of benthic diatoms to atrazine using rapid toxicity testing: A novel method. / Wood, Rebecca; Mitrovic, Simon; KEFFORD, Ben.

    In: Science of the Total Environment, Vol. 485-486, 2014, p. 421-427.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - Mitrovic, Simon

    AU - KEFFORD, Ben

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