Developing a sentinel mollusc species for toxicity assessment

Metal exposure, dose and response - laboratory v. field exposures and resident organisms

Anne TAYLOR, Bill MAHER

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Abstract. Relationships between exposure, tissue dose and biological responses of the benthic marine bivalve Anadara trapezia to an estuarine sediment zinc, copper, lead, cadmium and selenium contamination gradient in Lake Macquarie, NSW, were evaluated using three approaches. Organisms were exposed to sediments in laboratory aquaria, caged in situ in the lake and lake resident organisms collected. Dose included total metal tissue burden and subcellular metal distribution to determine metabolically available metal. Response indices were total antioxidant capacity, lipid peroxidation, lysosomal stability and condition index. Bioaccumulation of total metals was higher in the laboratory and resident organisms than in those transplanted in the field but the contribution of individual metals to the total differed. Laboratory-exposed organisms had increased concentrations of cadmium and lead in their biologically active and detoxified metal fractions but not of the essential elements zinc and copper. Subcellular metal distribution patterns were the same in resident organisms but cadmium and lead burdens were higher in both fractions. Biomarker responses were similar in laboratory, transplanted and resident organisms. Total antioxidant capacity was significantly reduced and lipid peroxidation and lysosomal destabilisation significantly increased in all metal-exposed organisms compared with the reference A. trapezia. Condition index of laboratory-exposed organisms was significantly lower than in situ, resident and reference organisms. Clear metal exposure¿dose¿response relationships have been demonstrated for A. trapezia in laboratory and in situ experiments. Non-resident organisms, in both exposure scenarios, gave similar responses to resident metal-exposed organisms, showing all approaches are valid when investigating effects in this species.
    Original languageEnglish
    Pages (from-to)434-446
    Number of pages13
    JournalEnvironmental Chemistry
    Volume13
    Issue number3
    DOIs
    Publication statusPublished - 2016

    Fingerprint

    Molluscs
    mollusc
    Toxicity
    Metals
    toxicity
    metal
    Cadmium
    Lakes
    cadmium
    Zinc
    Copper
    antioxidant
    Sediments
    Antioxidants
    lake
    cyhalothrin
    organism
    exposure
    laboratory
    dose

    Cite this

    @article{dccd05f14b174450b3a0ae38d3a4ce67,
    title = "Developing a sentinel mollusc species for toxicity assessment: Metal exposure, dose and response - laboratory v. field exposures and resident organisms",
    abstract = "Abstract. Relationships between exposure, tissue dose and biological responses of the benthic marine bivalve Anadara trapezia to an estuarine sediment zinc, copper, lead, cadmium and selenium contamination gradient in Lake Macquarie, NSW, were evaluated using three approaches. Organisms were exposed to sediments in laboratory aquaria, caged in situ in the lake and lake resident organisms collected. Dose included total metal tissue burden and subcellular metal distribution to determine metabolically available metal. Response indices were total antioxidant capacity, lipid peroxidation, lysosomal stability and condition index. Bioaccumulation of total metals was higher in the laboratory and resident organisms than in those transplanted in the field but the contribution of individual metals to the total differed. Laboratory-exposed organisms had increased concentrations of cadmium and lead in their biologically active and detoxified metal fractions but not of the essential elements zinc and copper. Subcellular metal distribution patterns were the same in resident organisms but cadmium and lead burdens were higher in both fractions. Biomarker responses were similar in laboratory, transplanted and resident organisms. Total antioxidant capacity was significantly reduced and lipid peroxidation and lysosomal destabilisation significantly increased in all metal-exposed organisms compared with the reference A. trapezia. Condition index of laboratory-exposed organisms was significantly lower than in situ, resident and reference organisms. Clear metal exposure¿dose¿response relationships have been demonstrated for A. trapezia in laboratory and in situ experiments. Non-resident organisms, in both exposure scenarios, gave similar responses to resident metal-exposed organisms, showing all approaches are valid when investigating effects in this species.",
    author = "Anne TAYLOR and Bill MAHER",
    year = "2016",
    doi = "10.1071/EN15104",
    language = "English",
    volume = "13",
    pages = "434--446",
    journal = "Environmental Chemistry",
    issn = "1448-2517",
    publisher = "CSIRO",
    number = "3",

    }

    TY - JOUR

    T1 - Developing a sentinel mollusc species for toxicity assessment

    T2 - Metal exposure, dose and response - laboratory v. field exposures and resident organisms

    AU - TAYLOR, Anne

    AU - MAHER, Bill

    PY - 2016

    Y1 - 2016

    N2 - Abstract. Relationships between exposure, tissue dose and biological responses of the benthic marine bivalve Anadara trapezia to an estuarine sediment zinc, copper, lead, cadmium and selenium contamination gradient in Lake Macquarie, NSW, were evaluated using three approaches. Organisms were exposed to sediments in laboratory aquaria, caged in situ in the lake and lake resident organisms collected. Dose included total metal tissue burden and subcellular metal distribution to determine metabolically available metal. Response indices were total antioxidant capacity, lipid peroxidation, lysosomal stability and condition index. Bioaccumulation of total metals was higher in the laboratory and resident organisms than in those transplanted in the field but the contribution of individual metals to the total differed. Laboratory-exposed organisms had increased concentrations of cadmium and lead in their biologically active and detoxified metal fractions but not of the essential elements zinc and copper. Subcellular metal distribution patterns were the same in resident organisms but cadmium and lead burdens were higher in both fractions. Biomarker responses were similar in laboratory, transplanted and resident organisms. Total antioxidant capacity was significantly reduced and lipid peroxidation and lysosomal destabilisation significantly increased in all metal-exposed organisms compared with the reference A. trapezia. Condition index of laboratory-exposed organisms was significantly lower than in situ, resident and reference organisms. Clear metal exposure¿dose¿response relationships have been demonstrated for A. trapezia in laboratory and in situ experiments. Non-resident organisms, in both exposure scenarios, gave similar responses to resident metal-exposed organisms, showing all approaches are valid when investigating effects in this species.

    AB - Abstract. Relationships between exposure, tissue dose and biological responses of the benthic marine bivalve Anadara trapezia to an estuarine sediment zinc, copper, lead, cadmium and selenium contamination gradient in Lake Macquarie, NSW, were evaluated using three approaches. Organisms were exposed to sediments in laboratory aquaria, caged in situ in the lake and lake resident organisms collected. Dose included total metal tissue burden and subcellular metal distribution to determine metabolically available metal. Response indices were total antioxidant capacity, lipid peroxidation, lysosomal stability and condition index. Bioaccumulation of total metals was higher in the laboratory and resident organisms than in those transplanted in the field but the contribution of individual metals to the total differed. Laboratory-exposed organisms had increased concentrations of cadmium and lead in their biologically active and detoxified metal fractions but not of the essential elements zinc and copper. Subcellular metal distribution patterns were the same in resident organisms but cadmium and lead burdens were higher in both fractions. Biomarker responses were similar in laboratory, transplanted and resident organisms. Total antioxidant capacity was significantly reduced and lipid peroxidation and lysosomal destabilisation significantly increased in all metal-exposed organisms compared with the reference A. trapezia. Condition index of laboratory-exposed organisms was significantly lower than in situ, resident and reference organisms. Clear metal exposure¿dose¿response relationships have been demonstrated for A. trapezia in laboratory and in situ experiments. Non-resident organisms, in both exposure scenarios, gave similar responses to resident metal-exposed organisms, showing all approaches are valid when investigating effects in this species.

    U2 - 10.1071/EN15104

    DO - 10.1071/EN15104

    M3 - Article

    VL - 13

    SP - 434

    EP - 446

    JO - Environmental Chemistry

    JF - Environmental Chemistry

    SN - 1448-2517

    IS - 3

    ER -