Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis

Wei Zhou, Geoffry N. De Iuliis, Adrian P. Turner, Andrew T. Reid, Amanda L. Anderson, Adam McCluskey, Eileen A. McLaughlin, Brett Nixon

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The mammalian epididymis is an exceptionally long ductal system tasked with the provision of one of the most complex intraluminal fluids found in any exocrine gland. This specialized milieu is continuously modified by the combined secretory and absorptive of the surrounding epithelium and thus finely tuned for its essential roles in promoting sperm maturation and storage. While considerable effort has been focused on defining the composition of the epididymal fluid, relatively less is known about the intracellular trafficking machinery that regulates this luminal environment. Here, we characterize the ontogeny of expression of a master regulator of this machinery, the dynamin family of mechanoenzymes. Our data show that canonical dynamin isoforms were abundantly expressed in the juvenile mouse epididymis. However, in peripubertal and adult animals dynamin takes on a heterogeneous pattern of expression such that the different isoforms displayed both cell- and segment-specific localization. Thus, dynamin 1 and 3 were predominately localized in the distal epididymal segments (corpus and cauda), where they were found within clear and principal cells, respectively. In contrast, dynamin 2 was expressed throughout the epididymis, but localized to the Golgi apparatus of the principal cells in the proximal (caput) segment and the luminal border of these cells in more distal segments. These dynamin isoforms are therefore ideally positioned to play complementary, nonredundant roles in the regulation of the epididymal milieu. In support of this hypothesis, selective inhibition of dynamin altered the profile of proteins secreted from an immortalized caput epididymal cell line.

Original languageEnglish
Article number145433
Pages (from-to)159-173
Number of pages15
JournalBiology of Reproduction
Volume96
Issue number1
Early online date20 Dec 2016
DOIs
Publication statusPublished - 1 Jan 2017
Externally publishedYes

Fingerprint

Dynamins
Epididymis
Protein Isoforms
Dynamin III
Dynamin I
Dynamin II
Exocrine Glands
Sperm Maturation
Golgi Apparatus
Epithelium
Cell Line
Proteins

Cite this

Zhou, W., De Iuliis, G. N., Turner, A. P., Reid, A. T., Anderson, A. L., McCluskey, A., ... Nixon, B. (2017). Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis. Biology of Reproduction, 96(1), 159-173. [145433]. https://doi.org/10.1095/biolreprod.116.145433
Zhou, Wei ; De Iuliis, Geoffry N. ; Turner, Adrian P. ; Reid, Andrew T. ; Anderson, Amanda L. ; McCluskey, Adam ; McLaughlin, Eileen A. ; Nixon, Brett. / Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis. In: Biology of Reproduction. 2017 ; Vol. 96, No. 1. pp. 159-173.
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Zhou, W, De Iuliis, GN, Turner, AP, Reid, AT, Anderson, AL, McCluskey, A, McLaughlin, EA & Nixon, B 2017, 'Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis', Biology of Reproduction, vol. 96, no. 1, 145433, pp. 159-173. https://doi.org/10.1095/biolreprod.116.145433

Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis. / Zhou, Wei; De Iuliis, Geoffry N.; Turner, Adrian P.; Reid, Andrew T.; Anderson, Amanda L.; McCluskey, Adam; McLaughlin, Eileen A.; Nixon, Brett.

In: Biology of Reproduction, Vol. 96, No. 1, 145433, 01.01.2017, p. 159-173.

Research output: Contribution to journalArticle

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Zhou W, De Iuliis GN, Turner AP, Reid AT, Anderson AL, McCluskey A et al. Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis. Biology of Reproduction. 2017 Jan 1;96(1):159-173. 145433. https://doi.org/10.1095/biolreprod.116.145433