Differences in hormone localisation patterns of K and L type enteroendocrine cells in the mouse and pig small intestine and colon

Hyunjung Cho, Sam KOSARI, Billie Hunne, Brid Callaghan, Leni Rivera, David Bravo, John Furness

Research output: Contribution to journalArticle

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Abstract

This study has investigated the patterns of colocalisation of the conventional K cell marker, glucagon-like insulinotropic peptide (GIP), and the L cell markers, glucagon like peptide-1 (GLP-1) and peptide YY (PYY), in enteroendocrine cells (EEC) of the small intestine and colon of mouse and pig. All combinations of the hormones, 3 in a cell, 2 in a cell and 1 at a time, were encountered. In both species, the three most common EEC types contained (1) both GLP-1 and PYY but not GIP, (2) GLP-1 alone or (3) GIP plus GLP-1 without PYY. Few GIP plus PYY cells and rare cells containing all 3 hormones were encountered. Gradients of cell types occurred along the intestine. For example, in mouse, there were no PYY cells in the duodenum and few in the jejunum, but >50 % of labelled EEC in the distal ileum and colon were PYY immunoreactive. By contrast, over 40 % of EEC in the pig duodenum contained PYY, and most also contained either GLP-1 or GIP. The gradient in pig was less pronounced. It is concluded that the traditional classification of K and L cells requires revision, and that there are major inter-species differences in the patterns of colocalisation of hormones that have been used to characterise K and L cells
Original languageEnglish
Pages (from-to)693-698
Number of pages6
JournalCell and Tissue Research
Volume359
Issue number2
DOIs
Publication statusPublished - 2015
Externally publishedYes

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Enteroendocrine Cells
Peptide YY
Small Intestine
Colon
Swine
Hormones
Glucagon-Like Peptide 1
Glucagon-Like Peptides
Duodenum
Glucagon-Like Peptide 2
Jejunum
Ileum
Intestines

Cite this

Cho, Hyunjung ; KOSARI, Sam ; Hunne, Billie ; Callaghan, Brid ; Rivera, Leni ; Bravo, David ; Furness, John. / Differences in hormone localisation patterns of K and L type enteroendocrine cells in the mouse and pig small intestine and colon. In: Cell and Tissue Research. 2015 ; Vol. 359, No. 2. pp. 693-698.
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abstract = "This study has investigated the patterns of colocalisation of the conventional K cell marker, glucagon-like insulinotropic peptide (GIP), and the L cell markers, glucagon like peptide-1 (GLP-1) and peptide YY (PYY), in enteroendocrine cells (EEC) of the small intestine and colon of mouse and pig. All combinations of the hormones, 3 in a cell, 2 in a cell and 1 at a time, were encountered. In both species, the three most common EEC types contained (1) both GLP-1 and PYY but not GIP, (2) GLP-1 alone or (3) GIP plus GLP-1 without PYY. Few GIP plus PYY cells and rare cells containing all 3 hormones were encountered. Gradients of cell types occurred along the intestine. For example, in mouse, there were no PYY cells in the duodenum and few in the jejunum, but >50 {\%} of labelled EEC in the distal ileum and colon were PYY immunoreactive. By contrast, over 40 {\%} of EEC in the pig duodenum contained PYY, and most also contained either GLP-1 or GIP. The gradient in pig was less pronounced. It is concluded that the traditional classification of K and L cells requires revision, and that there are major inter-species differences in the patterns of colocalisation of hormones that have been used to characterise K and L cells",
author = "Hyunjung Cho and Sam KOSARI and Billie Hunne and Brid Callaghan and Leni Rivera and David Bravo and John Furness",
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Differences in hormone localisation patterns of K and L type enteroendocrine cells in the mouse and pig small intestine and colon. / Cho, Hyunjung; KOSARI, Sam; Hunne, Billie; Callaghan, Brid; Rivera, Leni; Bravo, David; Furness, John.

In: Cell and Tissue Research, Vol. 359, No. 2, 2015, p. 693-698.

Research output: Contribution to journalArticle

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T1 - Differences in hormone localisation patterns of K and L type enteroendocrine cells in the mouse and pig small intestine and colon

AU - Cho, Hyunjung

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AU - Rivera, Leni

AU - Bravo, David

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AB - This study has investigated the patterns of colocalisation of the conventional K cell marker, glucagon-like insulinotropic peptide (GIP), and the L cell markers, glucagon like peptide-1 (GLP-1) and peptide YY (PYY), in enteroendocrine cells (EEC) of the small intestine and colon of mouse and pig. All combinations of the hormones, 3 in a cell, 2 in a cell and 1 at a time, were encountered. In both species, the three most common EEC types contained (1) both GLP-1 and PYY but not GIP, (2) GLP-1 alone or (3) GIP plus GLP-1 without PYY. Few GIP plus PYY cells and rare cells containing all 3 hormones were encountered. Gradients of cell types occurred along the intestine. For example, in mouse, there were no PYY cells in the duodenum and few in the jejunum, but >50 % of labelled EEC in the distal ileum and colon were PYY immunoreactive. By contrast, over 40 % of EEC in the pig duodenum contained PYY, and most also contained either GLP-1 or GIP. The gradient in pig was less pronounced. It is concluded that the traditional classification of K and L cells requires revision, and that there are major inter-species differences in the patterns of colocalisation of hormones that have been used to characterise K and L cells

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