Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 693-698 |
| Number of pages | 6 |
| Journal | Cell and Tissue Research |
| Volume | 359 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2015 |
| Externally published | Yes |
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Differences in hormone localisation patterns of K and L type enteroendocrine cells in the mouse and pig small intestine and colon. / Cho, Hyunjung; KOSARI, Sam; Hunne, Billie; Callaghan, Brid; Rivera, Leni; Bravo, David; Furness, John.
In: Cell and Tissue Research, Vol. 359, No. 2, 2015, p. 693-698.Research output: Contribution to journal › Article
TY - JOUR
T1 - Differences in hormone localisation patterns of K and L type enteroendocrine cells in the mouse and pig small intestine and colon
AU - Cho, Hyunjung
AU - KOSARI, Sam
AU - Hunne, Billie
AU - Callaghan, Brid
AU - Rivera, Leni
AU - Bravo, David
AU - Furness, John
PY - 2015
Y1 - 2015
N2 - This study has investigated the patterns of colocalisation of the conventional K cell marker, glucagon-like insulinotropic peptide (GIP), and the L cell markers, glucagon like peptide-1 (GLP-1) and peptide YY (PYY), in enteroendocrine cells (EEC) of the small intestine and colon of mouse and pig. All combinations of the hormones, 3 in a cell, 2 in a cell and 1 at a time, were encountered. In both species, the three most common EEC types contained (1) both GLP-1 and PYY but not GIP, (2) GLP-1 alone or (3) GIP plus GLP-1 without PYY. Few GIP plus PYY cells and rare cells containing all 3 hormones were encountered. Gradients of cell types occurred along the intestine. For example, in mouse, there were no PYY cells in the duodenum and few in the jejunum, but >50 % of labelled EEC in the distal ileum and colon were PYY immunoreactive. By contrast, over 40 % of EEC in the pig duodenum contained PYY, and most also contained either GLP-1 or GIP. The gradient in pig was less pronounced. It is concluded that the traditional classification of K and L cells requires revision, and that there are major inter-species differences in the patterns of colocalisation of hormones that have been used to characterise K and L cells
AB - This study has investigated the patterns of colocalisation of the conventional K cell marker, glucagon-like insulinotropic peptide (GIP), and the L cell markers, glucagon like peptide-1 (GLP-1) and peptide YY (PYY), in enteroendocrine cells (EEC) of the small intestine and colon of mouse and pig. All combinations of the hormones, 3 in a cell, 2 in a cell and 1 at a time, were encountered. In both species, the three most common EEC types contained (1) both GLP-1 and PYY but not GIP, (2) GLP-1 alone or (3) GIP plus GLP-1 without PYY. Few GIP plus PYY cells and rare cells containing all 3 hormones were encountered. Gradients of cell types occurred along the intestine. For example, in mouse, there were no PYY cells in the duodenum and few in the jejunum, but >50 % of labelled EEC in the distal ileum and colon were PYY immunoreactive. By contrast, over 40 % of EEC in the pig duodenum contained PYY, and most also contained either GLP-1 or GIP. The gradient in pig was less pronounced. It is concluded that the traditional classification of K and L cells requires revision, and that there are major inter-species differences in the patterns of colocalisation of hormones that have been used to characterise K and L cells
KW - Gastrointestinal hormones
KW - Glucagon like peptide
KW - Glucagon-like insulinotropic peptide
KW - K cells
KW - L cells
KW - Peptide YY
UR - http://www.scopus.com/inward/record.url?scp=84925513501&partnerID=8YFLogxK
U2 - 10.1007/s00441-014-2033-3
DO - 10.1007/s00441-014-2033-3
M3 - Article
VL - 359
SP - 693
EP - 698
JO - Zeitschrift für Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948)
JF - Zeitschrift für Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948)
SN - 0302-766X
IS - 2
ER -