Differential Roles of HOW in Male and Female Drosophila Germline Differentiation

Adrian C. Monk, Nicole A. Siddall, Barbara Fraser, Eileen A. McLaughlin, Gary R. Hime

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The adult gonads in both male and female Drosophila melanogaster produce gametes that originate from a regenerative pool of germline stem cells (GSCs). The differentiation programme that produces gametes must be co-ordinated with GSC maintenance and proliferation in order to regulate tissue regeneration. The HOW RNA-binding protein has been shown to maintain mitotic progression of male GSCs and their daughters by maintenance of Cyclin B expression as well as suppressing accumulation of the differentiation factor Bam. Loss of HOW function in the male germline results in loss of GSCs due to a delay in G2 and subsequent apoptosis. Here we show that female how mutant GSCs do not have any cell cycle defects although HOW continues to bind bam mRNA and suppress Bam expression. The role of HOW in suppressing germ cell Bam expression appears to be conserved between sexes, leading to different cellular outcomes in how mutants due to the different functions of Bam. In addition the role in maintaining Cyclin B expression has not been conserved so female how GSCs differentiate rather than arrest.

Original languageEnglish
Article numbere28508
Pages (from-to)1-12
Number of pages12
JournalPLoS One
Volume6
Issue number12
DOIs
Publication statusPublished - 6 Dec 2011
Externally publishedYes

Fingerprint

Stem cells
Drosophila
germ cells
Stem Cells
Germ Cells
Cyclin B
stem cells
Maintenance
RNA-Binding Proteins
Gonads
Drosophila melanogaster
Cells
cyclins
Regeneration
Tissue regeneration
Cell Cycle
Cell Proliferation
Apoptosis
Messenger RNA
mutants

Cite this

Monk, A. C., Siddall, N. A., Fraser, B., McLaughlin, E. A., & Hime, G. R. (2011). Differential Roles of HOW in Male and Female Drosophila Germline Differentiation. PLoS One, 6(12), 1-12. [e28508]. https://doi.org/10.1371/journal.pone.0028508
Monk, Adrian C. ; Siddall, Nicole A. ; Fraser, Barbara ; McLaughlin, Eileen A. ; Hime, Gary R. / Differential Roles of HOW in Male and Female Drosophila Germline Differentiation. In: PLoS One. 2011 ; Vol. 6, No. 12. pp. 1-12.
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Monk, AC, Siddall, NA, Fraser, B, McLaughlin, EA & Hime, GR 2011, 'Differential Roles of HOW in Male and Female Drosophila Germline Differentiation', PLoS One, vol. 6, no. 12, e28508, pp. 1-12. https://doi.org/10.1371/journal.pone.0028508

Differential Roles of HOW in Male and Female Drosophila Germline Differentiation. / Monk, Adrian C.; Siddall, Nicole A.; Fraser, Barbara; McLaughlin, Eileen A.; Hime, Gary R.

In: PLoS One, Vol. 6, No. 12, e28508, 06.12.2011, p. 1-12.

Research output: Contribution to journalArticle

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Monk AC, Siddall NA, Fraser B, McLaughlin EA, Hime GR. Differential Roles of HOW in Male and Female Drosophila Germline Differentiation. PLoS One. 2011 Dec 6;6(12):1-12. e28508. https://doi.org/10.1371/journal.pone.0028508