Direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The solvent versatility of Chiralpak IB, a 3,5‐dimethylphenylcarbamate derivative of cellulose‐based chiral stationary phase, is demonstrated in the direct enantioselective HPLC monitoring of lipase‐catalyzed kinetic resolution of flurbiprofen in nonstandard HPLC organic solvents. Nonstandard HPLC organic solvents were used as the reaction media for the lipase‐catalysis and in mean time as diluent to dissolve the “difficult to dissolve” enzyme substrate (the acid) and as eluent for the simultaneous enantioselective HPLC baseline separation of both substrate and product in one run without any further derivatization
Original languageEnglish
Pages (from-to)597-603
Number of pages7
JournalChirality
Volume22
Issue number6
DOIs
Publication statusPublished - 2010

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Flurbiprofen
Enzyme kinetics
Lipases
Lipase
Organic solvents
High Pressure Liquid Chromatography
Kinetics
Monitoring
Substrates
Enzymes
Derivatives
Acids

Cite this

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title = "Direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen",
abstract = "The solvent versatility of Chiralpak IB, a 3,5‐dimethylphenylcarbamate derivative of cellulose‐based chiral stationary phase, is demonstrated in the direct enantioselective HPLC monitoring of lipase‐catalyzed kinetic resolution of flurbiprofen in nonstandard HPLC organic solvents. Nonstandard HPLC organic solvents were used as the reaction media for the lipase‐catalysis and in mean time as diluent to dissolve the “difficult to dissolve” enzyme substrate (the acid) and as eluent for the simultaneous enantioselective HPLC baseline separation of both substrate and product in one run without any further derivatization",
author = "Ashraf Ghanem",
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Direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen. / Ghanem, Ashraf.

In: Chirality, Vol. 22, No. 6, 2010, p. 597-603.

Research output: Contribution to journalArticle

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T1 - Direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen

AU - Ghanem, Ashraf

PY - 2010

Y1 - 2010

N2 - The solvent versatility of Chiralpak IB, a 3,5‐dimethylphenylcarbamate derivative of cellulose‐based chiral stationary phase, is demonstrated in the direct enantioselective HPLC monitoring of lipase‐catalyzed kinetic resolution of flurbiprofen in nonstandard HPLC organic solvents. Nonstandard HPLC organic solvents were used as the reaction media for the lipase‐catalysis and in mean time as diluent to dissolve the “difficult to dissolve” enzyme substrate (the acid) and as eluent for the simultaneous enantioselective HPLC baseline separation of both substrate and product in one run without any further derivatization

AB - The solvent versatility of Chiralpak IB, a 3,5‐dimethylphenylcarbamate derivative of cellulose‐based chiral stationary phase, is demonstrated in the direct enantioselective HPLC monitoring of lipase‐catalyzed kinetic resolution of flurbiprofen in nonstandard HPLC organic solvents. Nonstandard HPLC organic solvents were used as the reaction media for the lipase‐catalysis and in mean time as diluent to dissolve the “difficult to dissolve” enzyme substrate (the acid) and as eluent for the simultaneous enantioselective HPLC baseline separation of both substrate and product in one run without any further derivatization

U2 - 10.1002/chir.20798

DO - 10.1002/chir.20798

M3 - Article

VL - 22

SP - 597

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JO - Chirality

JF - Chirality

SN - 0899-0042

IS - 6

ER -