Direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen

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Abstract

The solvent versatility of Chiralpak IB, a 3,5‐dimethylphenylcarbamate derivative of cellulose‐based chiral stationary phase, is demonstrated in the direct enantioselective HPLC monitoring of lipase‐catalyzed kinetic resolution of flurbiprofen in nonstandard HPLC organic solvents. Nonstandard HPLC organic solvents were used as the reaction media for the lipase‐catalysis and in mean time as diluent to dissolve the “difficult to dissolve” enzyme substrate (the acid) and as eluent for the simultaneous enantioselective HPLC baseline separation of both substrate and product in one run without any further derivatization
Original languageEnglish
Pages (from-to)597-603
Number of pages7
JournalChirality
Volume22
Issue number6
DOIs
Publication statusPublished - 2010

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