Direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen

Ashraf Ghanem

doi:10.1002/chir.20798

Direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen

Ashraf Ghanem

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The solvent versatility of Chiralpak IB, a 3,5‐dimethylphenylcarbamate derivative of cellulose‐based chiral stationary phase, is demonstrated in the direct enantioselective HPLC monitoring of lipase‐catalyzed kinetic resolution of flurbiprofen in nonstandard HPLC organic solvents. Nonstandard HPLC organic solvents were used as the reaction media for the lipase‐catalysis and in mean time as diluent to dissolve the “difficult to dissolve” enzyme substrate (the acid) and as eluent for the simultaneous enantioselective HPLC baseline separation of both substrate and product in one run without any further derivatization

Original language	English
Pages (from-to)	597-603
Number of pages	7
Journal	Chirality
Volume	22
Issue number	6
DOIs	https://doi.org/10.1002/chir.20798
Publication status	Published - 2010

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10.1002/chir.20798

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title = "Direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen",

abstract = "The solvent versatility of Chiralpak IB, a 3,5‐dimethylphenylcarbamate derivative of cellulose‐based chiral stationary phase, is demonstrated in the direct enantioselective HPLC monitoring of lipase‐catalyzed kinetic resolution of flurbiprofen in nonstandard HPLC organic solvents. Nonstandard HPLC organic solvents were used as the reaction media for the lipase‐catalysis and in mean time as diluent to dissolve the “difficult to dissolve” enzyme substrate (the acid) and as eluent for the simultaneous enantioselective HPLC baseline separation of both substrate and product in one run without any further derivatization",

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AU - Ghanem, Ashraf

PY - 2010

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N2 - The solvent versatility of Chiralpak IB, a 3,5‐dimethylphenylcarbamate derivative of cellulose‐based chiral stationary phase, is demonstrated in the direct enantioselective HPLC monitoring of lipase‐catalyzed kinetic resolution of flurbiprofen in nonstandard HPLC organic solvents. Nonstandard HPLC organic solvents were used as the reaction media for the lipase‐catalysis and in mean time as diluent to dissolve the “difficult to dissolve” enzyme substrate (the acid) and as eluent for the simultaneous enantioselective HPLC baseline separation of both substrate and product in one run without any further derivatization

AB - The solvent versatility of Chiralpak IB, a 3,5‐dimethylphenylcarbamate derivative of cellulose‐based chiral stationary phase, is demonstrated in the direct enantioselective HPLC monitoring of lipase‐catalyzed kinetic resolution of flurbiprofen in nonstandard HPLC organic solvents. Nonstandard HPLC organic solvents were used as the reaction media for the lipase‐catalysis and in mean time as diluent to dissolve the “difficult to dissolve” enzyme substrate (the acid) and as eluent for the simultaneous enantioselective HPLC baseline separation of both substrate and product in one run without any further derivatization

U2 - 10.1002/chir.20798

DO - 10.1002/chir.20798

M3 - Article

SN - 0899-0042

VL - 22

SP - 597

EP - 603

JO - Chirality

JF - Chirality

IS - 6

ER -

Direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen

Abstract

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