Direct interaction between alpha-actinin and hepatitis C virus NS5B

Shuiyun Lan, Hua Wang, Hong Jiang, Hongxia Mao, Xiaoying Liu, Xiaonan Zhang, Yunwen Hu, Li Xiang, Zhenghong Yuan

Research output: Contribution to journalReview articlepeer-review

27 Citations (Scopus)


It has been suggested that cellular proteins are involved in hepatitis C virus (HCV) RNA replication. By using the yeast two-hybrid system, we isolated seven cDNA clones encoding proteins interacting with HCV RNA polymerase (NS5B) from a human liver cDNA library. For one of these, alpha-actinin, we confirmed the interaction by coimmunoprecipitation, immunofluorescent staining and confocal microscopic analysis. Experiments with deletion mutants showed that domains NS5B(84-95), NS5B(466-478), and alpha-actinin(621-733) are responsible for the interaction. Studies of the HCV subgenomic replicon system with small interference RNA indicate that alpha-actinin is essential for HCV RNA replication. Our results suggest alpha-actinin may be a component of the HCV replication complex.

Original languageEnglish
Pages (from-to)289-294
Number of pages6
JournalFEBS Letters
Issue number3
Publication statusPublished - 20 Nov 2003
Externally publishedYes


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