Direct interaction between alpha-actinin and hepatitis C virus NS5B

Shuiyun Lan; Hua Wang; Hong Jiang; Hongxia Mao; Xiaoying Liu; Xiaonan Zhang; Yunwen Hu; Li Xiang; Zhenghong Yuan

doi:10.1016/s0014-5793(03)01163-3

Direct interaction between alpha-actinin and hepatitis C virus NS5B

Shuiyun Lan, Hua Wang, Hong Jiang, Hongxia Mao, Xiaoying Liu, Xiaonan Zhang, Yunwen Hu, Li Xiang, Zhenghong Yuan

Research output: Contribution to journal › Review article › peer-review

27 Citations (Scopus)

Abstract

It has been suggested that cellular proteins are involved in hepatitis C virus (HCV) RNA replication. By using the yeast two-hybrid system, we isolated seven cDNA clones encoding proteins interacting with HCV RNA polymerase (NS5B) from a human liver cDNA library. For one of these, alpha-actinin, we confirmed the interaction by coimmunoprecipitation, immunofluorescent staining and confocal microscopic analysis. Experiments with deletion mutants showed that domains NS5B(84-95), NS5B(466-478), and alpha-actinin(621-733) are responsible for the interaction. Studies of the HCV subgenomic replicon system with small interference RNA indicate that alpha-actinin is essential for HCV RNA replication. Our results suggest alpha-actinin may be a component of the HCV replication complex.

Original language	English
Pages (from-to)	289-294
Number of pages	6
Journal	FEBS Letters
Volume	554
Issue number	3
DOIs	https://doi.org/10.1016/s0014-5793(03)01163-3
Publication status	Published - 20 Nov 2003
Externally published	Yes

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10.1016/s0014-5793(03)01163-3

Cite this

@article{06ff94f77a3d43bc92659d71c9c133d8,

title = "Direct interaction between alpha-actinin and hepatitis C virus NS5B",

abstract = "It has been suggested that cellular proteins are involved in hepatitis C virus (HCV) RNA replication. By using the yeast two-hybrid system, we isolated seven cDNA clones encoding proteins interacting with HCV RNA polymerase (NS5B) from a human liver cDNA library. For one of these, alpha-actinin, we confirmed the interaction by coimmunoprecipitation, immunofluorescent staining and confocal microscopic analysis. Experiments with deletion mutants showed that domains NS5B(84-95), NS5B(466-478), and alpha-actinin(621-733) are responsible for the interaction. Studies of the HCV subgenomic replicon system with small interference RNA indicate that alpha-actinin is essential for HCV RNA replication. Our results suggest alpha-actinin may be a component of the HCV replication complex.",

keywords = "Actinin/chemistry, Animals, COS Cells, Cell Line, Tumor, Chlorocebus aethiops, DNA-Directed RNA Polymerases/chemistry, Fluorescent Antibody Technique, Gene Expression Regulation/drug effects, Gene Library, Hepacivirus/genetics, Humans, Microscopy, Confocal, Precipitin Tests, Protein Structure, Tertiary, RNA, Small Interfering/genetics, RNA, Viral/biosynthesis, Replicon/genetics, Sequence Deletion, Two-Hybrid System Techniques, Virus Replication/drug effects",

author = "Shuiyun Lan and Hua Wang and Hong Jiang and Hongxia Mao and Xiaoying Liu and Xiaonan Zhang and Yunwen Hu and Li Xiang and Zhenghong Yuan",

year = "2003",

month = nov,

day = "20",

doi = "10.1016/s0014-5793(03)01163-3",

language = "English",

volume = "554",

pages = "289--294",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Direct interaction between alpha-actinin and hepatitis C virus NS5B

AU - Lan, Shuiyun

AU - Wang, Hua

AU - Jiang, Hong

AU - Mao, Hongxia

AU - Liu, Xiaoying

AU - Zhang, Xiaonan

AU - Hu, Yunwen

AU - Xiang, Li

AU - Yuan, Zhenghong

PY - 2003/11/20

Y1 - 2003/11/20

N2 - It has been suggested that cellular proteins are involved in hepatitis C virus (HCV) RNA replication. By using the yeast two-hybrid system, we isolated seven cDNA clones encoding proteins interacting with HCV RNA polymerase (NS5B) from a human liver cDNA library. For one of these, alpha-actinin, we confirmed the interaction by coimmunoprecipitation, immunofluorescent staining and confocal microscopic analysis. Experiments with deletion mutants showed that domains NS5B(84-95), NS5B(466-478), and alpha-actinin(621-733) are responsible for the interaction. Studies of the HCV subgenomic replicon system with small interference RNA indicate that alpha-actinin is essential for HCV RNA replication. Our results suggest alpha-actinin may be a component of the HCV replication complex.

AB - It has been suggested that cellular proteins are involved in hepatitis C virus (HCV) RNA replication. By using the yeast two-hybrid system, we isolated seven cDNA clones encoding proteins interacting with HCV RNA polymerase (NS5B) from a human liver cDNA library. For one of these, alpha-actinin, we confirmed the interaction by coimmunoprecipitation, immunofluorescent staining and confocal microscopic analysis. Experiments with deletion mutants showed that domains NS5B(84-95), NS5B(466-478), and alpha-actinin(621-733) are responsible for the interaction. Studies of the HCV subgenomic replicon system with small interference RNA indicate that alpha-actinin is essential for HCV RNA replication. Our results suggest alpha-actinin may be a component of the HCV replication complex.

KW - Actinin/chemistry

KW - Animals

KW - COS Cells

KW - Cell Line, Tumor

KW - Chlorocebus aethiops

KW - DNA-Directed RNA Polymerases/chemistry

KW - Fluorescent Antibody Technique

KW - Gene Expression Regulation/drug effects

KW - Gene Library

KW - Hepacivirus/genetics

KW - Humans

KW - Microscopy, Confocal

KW - Precipitin Tests

KW - Protein Structure, Tertiary

KW - RNA, Small Interfering/genetics

KW - RNA, Viral/biosynthesis

KW - Replicon/genetics

KW - Sequence Deletion

KW - Two-Hybrid System Techniques

KW - Virus Replication/drug effects

U2 - 10.1016/s0014-5793(03)01163-3

DO - 10.1016/s0014-5793(03)01163-3

M3 - Review article

C2 - 14623081

SN - 0014-5793

VL - 554

SP - 289

EP - 294

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -