Do fertilin β and cyritestin play a major role in mammalian sperm-oolemma interactions? A critical re-evaluation of the use of peptide mimics in identifying specific oocyte recognition proteins

Eileen A. McLaughlin, Jan Frayne, Graham Bloomerg, Len Hall

Research output: Contribution to journalComment/debate

22 Citations (Scopus)


Integrins have been proposed to play a role in mammalian sperm-oocyte interactions for many years. To a large extent this hypothesis stems from the ability of short synthetic peptides, based on the disintegrin-like domains of two sperm surface integral membrane proteins, fertilin β and cyritestin, to inhibit sperm-oocyte binding and fusion in vitro. Here we argue that such peptide mimics lack specificity in these simple IVF assay systems. Hence, whilst not precluding a role for fertilin β and cyritestin in sperm-oolemma interactions, this lack of specificity indicates the need for considerable caution when interpreting results obtained using this approach.

Original languageEnglish
Pages (from-to)313-317
Number of pages5
JournalMolecular Human Reproduction
Issue number4
Publication statusPublished - 1 Jan 2001
Externally publishedYes


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