Do fertilin β and cyritestin play a major role in mammalian sperm-oolemma interactions? A critical re-evaluation of the use of peptide mimics in identifying specific oocyte recognition proteins

Eileen A. McLaughlin, Jan Frayne, Graham Bloomerg, Len Hall

Research output: Contribution to journalComment/debate

22 Citations (Scopus)

Abstract

Integrins have been proposed to play a role in mammalian sperm-oocyte interactions for many years. To a large extent this hypothesis stems from the ability of short synthetic peptides, based on the disintegrin-like domains of two sperm surface integral membrane proteins, fertilin β and cyritestin, to inhibit sperm-oocyte binding and fusion in vitro. Here we argue that such peptide mimics lack specificity in these simple IVF assay systems. Hence, whilst not precluding a role for fertilin β and cyritestin in sperm-oolemma interactions, this lack of specificity indicates the need for considerable caution when interpreting results obtained using this approach.

Original languageEnglish
Pages (from-to)313-317
Number of pages5
JournalMolecular Human Reproduction
Volume7
Issue number4
Publication statusPublished - 1 Jan 2001
Externally publishedYes

Fingerprint

Oocytes
Spermatozoa
Peptides
Sperm-Ovum Interactions
Disintegrins
Proteins
Integrins
Membrane Proteins
Fertilins
In Vitro Techniques

Cite this

@article{554d947d8a9940ffb2e126f0c8accbf2,
title = "Do fertilin β and cyritestin play a major role in mammalian sperm-oolemma interactions? A critical re-evaluation of the use of peptide mimics in identifying specific oocyte recognition proteins",
abstract = "Integrins have been proposed to play a role in mammalian sperm-oocyte interactions for many years. To a large extent this hypothesis stems from the ability of short synthetic peptides, based on the disintegrin-like domains of two sperm surface integral membrane proteins, fertilin β and cyritestin, to inhibit sperm-oocyte binding and fusion in vitro. Here we argue that such peptide mimics lack specificity in these simple IVF assay systems. Hence, whilst not precluding a role for fertilin β and cyritestin in sperm-oolemma interactions, this lack of specificity indicates the need for considerable caution when interpreting results obtained using this approach.",
keywords = "ADAM protein, Disintegrin, Integrin, IVF, MDC protein, Sperm-oocyte binding",
author = "McLaughlin, {Eileen A.} and Jan Frayne and Graham Bloomerg and Len Hall",
year = "2001",
month = "1",
day = "1",
language = "English",
volume = "7",
pages = "313--317",
journal = "Molecular Human Reproduction",
issn = "1360-9947",
publisher = "Oxford University Press",
number = "4",

}

Do fertilin β and cyritestin play a major role in mammalian sperm-oolemma interactions? A critical re-evaluation of the use of peptide mimics in identifying specific oocyte recognition proteins. / McLaughlin, Eileen A.; Frayne, Jan; Bloomerg, Graham; Hall, Len.

In: Molecular Human Reproduction, Vol. 7, No. 4, 01.01.2001, p. 313-317.

Research output: Contribution to journalComment/debate

TY - JOUR

T1 - Do fertilin β and cyritestin play a major role in mammalian sperm-oolemma interactions? A critical re-evaluation of the use of peptide mimics in identifying specific oocyte recognition proteins

AU - McLaughlin, Eileen A.

AU - Frayne, Jan

AU - Bloomerg, Graham

AU - Hall, Len

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Integrins have been proposed to play a role in mammalian sperm-oocyte interactions for many years. To a large extent this hypothesis stems from the ability of short synthetic peptides, based on the disintegrin-like domains of two sperm surface integral membrane proteins, fertilin β and cyritestin, to inhibit sperm-oocyte binding and fusion in vitro. Here we argue that such peptide mimics lack specificity in these simple IVF assay systems. Hence, whilst not precluding a role for fertilin β and cyritestin in sperm-oolemma interactions, this lack of specificity indicates the need for considerable caution when interpreting results obtained using this approach.

AB - Integrins have been proposed to play a role in mammalian sperm-oocyte interactions for many years. To a large extent this hypothesis stems from the ability of short synthetic peptides, based on the disintegrin-like domains of two sperm surface integral membrane proteins, fertilin β and cyritestin, to inhibit sperm-oocyte binding and fusion in vitro. Here we argue that such peptide mimics lack specificity in these simple IVF assay systems. Hence, whilst not precluding a role for fertilin β and cyritestin in sperm-oolemma interactions, this lack of specificity indicates the need for considerable caution when interpreting results obtained using this approach.

KW - ADAM protein

KW - Disintegrin

KW - Integrin

KW - IVF

KW - MDC protein

KW - Sperm-oocyte binding

UR - http://www.scopus.com/inward/record.url?scp=0035070684&partnerID=8YFLogxK

M3 - Comment/debate

VL - 7

SP - 313

EP - 317

JO - Molecular Human Reproduction

JF - Molecular Human Reproduction

SN - 1360-9947

IS - 4

ER -