Drosophila Rbp6 Is an Orthologue of Vertebrate Msi-1 and Msi-2, but Does Not Function Redundantly with dMsi to Regulate Germline Stem Cell Behaviour

Nicole A. Siddall, Marina Kalcina, Timothy M. Johanson, Adrian C. Monk, Franca Casagranda, Reeva P. Been, Eileen A. McLaughlin, Gary R. Hime

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The vertebrate RNA-binding proteins, Musashi-1 (Msi-1) and Musashi-2 (Msi-2) are expressed in multiple stem cell populations. A role for Musashi proteins in preventing stem cell differentiation has been suggested from genetic analysis of the Drosophila family member, dMsi, and both vertebrate Msi proteins function co-operatively to regulate neural stem cell behaviour. Here we have identified a second Drosophila Msi family member, Rbp6, which shares more amino acid identity with vertebrate Msi-1 and Msi-2 than dMsi. We generated an antibody that detects most Rbp6 splice isoforms and show that Rbp6 is expressed in multiple tissues throughout development. However, Rbp6 deletion mutants generated in this study are viable and fertile, and show only minor defects. We used Drosophila spermatogonial germline stem cells (GSC's) as a model to test whether Drosophila Msi proteins function redundantly to regulate stem cell behaviour. However, like vertebrate Msi-1 and Msi-2, Rbp6 and Msi do not appear to be co-expressed in spermatogenic GSC's and do not function co-operatively in the regulation of GSC maintenance. Thus while two Msi family members are present in Drosophila, the function of the family members have diverged.

Original languageEnglish
Article numbere49810
Pages (from-to)1-13
Number of pages13
JournalPLoS One
Volume7
Issue number11
DOIs
Publication statusPublished - 27 Nov 2012
Externally publishedYes

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Stem cells
Drosophila
Vertebrates
stem cells
germ cells
Stem Cells
vertebrates
RNA-Binding Proteins
Neural Stem Cells
RNA-binding proteins
proteins
Cell Differentiation
Protein Isoforms
cell differentiation
Proteins
genetic techniques and protocols
Maintenance
Amino Acids
Tissue
Antibodies

Cite this

Siddall, N. A., Kalcina, M., Johanson, T. M., Monk, A. C., Casagranda, F., Been, R. P., ... Hime, G. R. (2012). Drosophila Rbp6 Is an Orthologue of Vertebrate Msi-1 and Msi-2, but Does Not Function Redundantly with dMsi to Regulate Germline Stem Cell Behaviour. PLoS One, 7(11), 1-13. [e49810]. https://doi.org/10.1371/journal.pone.0049810
Siddall, Nicole A. ; Kalcina, Marina ; Johanson, Timothy M. ; Monk, Adrian C. ; Casagranda, Franca ; Been, Reeva P. ; McLaughlin, Eileen A. ; Hime, Gary R. / Drosophila Rbp6 Is an Orthologue of Vertebrate Msi-1 and Msi-2, but Does Not Function Redundantly with dMsi to Regulate Germline Stem Cell Behaviour. In: PLoS One. 2012 ; Vol. 7, No. 11. pp. 1-13.
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abstract = "The vertebrate RNA-binding proteins, Musashi-1 (Msi-1) and Musashi-2 (Msi-2) are expressed in multiple stem cell populations. A role for Musashi proteins in preventing stem cell differentiation has been suggested from genetic analysis of the Drosophila family member, dMsi, and both vertebrate Msi proteins function co-operatively to regulate neural stem cell behaviour. Here we have identified a second Drosophila Msi family member, Rbp6, which shares more amino acid identity with vertebrate Msi-1 and Msi-2 than dMsi. We generated an antibody that detects most Rbp6 splice isoforms and show that Rbp6 is expressed in multiple tissues throughout development. However, Rbp6 deletion mutants generated in this study are viable and fertile, and show only minor defects. We used Drosophila spermatogonial germline stem cells (GSC's) as a model to test whether Drosophila Msi proteins function redundantly to regulate stem cell behaviour. However, like vertebrate Msi-1 and Msi-2, Rbp6 and Msi do not appear to be co-expressed in spermatogenic GSC's and do not function co-operatively in the regulation of GSC maintenance. Thus while two Msi family members are present in Drosophila, the function of the family members have diverged.",
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Siddall, NA, Kalcina, M, Johanson, TM, Monk, AC, Casagranda, F, Been, RP, McLaughlin, EA & Hime, GR 2012, 'Drosophila Rbp6 Is an Orthologue of Vertebrate Msi-1 and Msi-2, but Does Not Function Redundantly with dMsi to Regulate Germline Stem Cell Behaviour', PLoS One, vol. 7, no. 11, e49810, pp. 1-13. https://doi.org/10.1371/journal.pone.0049810

Drosophila Rbp6 Is an Orthologue of Vertebrate Msi-1 and Msi-2, but Does Not Function Redundantly with dMsi to Regulate Germline Stem Cell Behaviour. / Siddall, Nicole A.; Kalcina, Marina; Johanson, Timothy M.; Monk, Adrian C.; Casagranda, Franca; Been, Reeva P.; McLaughlin, Eileen A.; Hime, Gary R.

In: PLoS One, Vol. 7, No. 11, e49810, 27.11.2012, p. 1-13.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Drosophila Rbp6 Is an Orthologue of Vertebrate Msi-1 and Msi-2, but Does Not Function Redundantly with dMsi to Regulate Germline Stem Cell Behaviour

AU - Siddall, Nicole A.

AU - Kalcina, Marina

AU - Johanson, Timothy M.

AU - Monk, Adrian C.

AU - Casagranda, Franca

AU - Been, Reeva P.

AU - McLaughlin, Eileen A.

AU - Hime, Gary R.

PY - 2012/11/27

Y1 - 2012/11/27

N2 - The vertebrate RNA-binding proteins, Musashi-1 (Msi-1) and Musashi-2 (Msi-2) are expressed in multiple stem cell populations. A role for Musashi proteins in preventing stem cell differentiation has been suggested from genetic analysis of the Drosophila family member, dMsi, and both vertebrate Msi proteins function co-operatively to regulate neural stem cell behaviour. Here we have identified a second Drosophila Msi family member, Rbp6, which shares more amino acid identity with vertebrate Msi-1 and Msi-2 than dMsi. We generated an antibody that detects most Rbp6 splice isoforms and show that Rbp6 is expressed in multiple tissues throughout development. However, Rbp6 deletion mutants generated in this study are viable and fertile, and show only minor defects. We used Drosophila spermatogonial germline stem cells (GSC's) as a model to test whether Drosophila Msi proteins function redundantly to regulate stem cell behaviour. However, like vertebrate Msi-1 and Msi-2, Rbp6 and Msi do not appear to be co-expressed in spermatogenic GSC's and do not function co-operatively in the regulation of GSC maintenance. Thus while two Msi family members are present in Drosophila, the function of the family members have diverged.

AB - The vertebrate RNA-binding proteins, Musashi-1 (Msi-1) and Musashi-2 (Msi-2) are expressed in multiple stem cell populations. A role for Musashi proteins in preventing stem cell differentiation has been suggested from genetic analysis of the Drosophila family member, dMsi, and both vertebrate Msi proteins function co-operatively to regulate neural stem cell behaviour. Here we have identified a second Drosophila Msi family member, Rbp6, which shares more amino acid identity with vertebrate Msi-1 and Msi-2 than dMsi. We generated an antibody that detects most Rbp6 splice isoforms and show that Rbp6 is expressed in multiple tissues throughout development. However, Rbp6 deletion mutants generated in this study are viable and fertile, and show only minor defects. We used Drosophila spermatogonial germline stem cells (GSC's) as a model to test whether Drosophila Msi proteins function redundantly to regulate stem cell behaviour. However, like vertebrate Msi-1 and Msi-2, Rbp6 and Msi do not appear to be co-expressed in spermatogenic GSC's and do not function co-operatively in the regulation of GSC maintenance. Thus while two Msi family members are present in Drosophila, the function of the family members have diverged.

KW - RNA binding proteins

KW - Stem Cells

KW - Neoplastic Stem Cells

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DO - 10.1371/journal.pone.0049810

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