Dynamic interplay of innate and adaptive immunity during sterile retinal inflammation: Insights from the transcriptome

Riccardo Natoli, Elizabeth Mason, Haihan Jiao, Aaron Chuah, Hardip Patel, Nilisha Fernando, Krisztina Valter, Christine A. Wells, Jan Provis, Matt Rutar

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)
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Abstract

The pathogenesis of many retinal degenerations, such as age-related macular degeneration (AMD), is punctuated by an ill-defined network of sterile inflammatory responses. The delineation of innate and adaptive immune milieu among the broad leukocyte infiltrate, and the gene networks, which construct these responses, are poorly described in the eye. Using photo-oxidative damage in a rodent model of subretinal inflammation, we employed a novel RNA-sequencing framework to map the global gene network signature of retinal leukocytes. This revealed a previously uncharted interplay of adaptive immunity during subretinal inflammation, including prolonged enrichment of myeloid and lymphocyte migration, antigen presentation, and the alternative arm of the complement cascade involving Factor B. We demonstrate Factor B-deficient mice are protected against macrophage infiltration and subretinal inflammation. Suppressing the drivers of retinal leukocyte proliferation, or their capacity to elicit complement responses, may help preserve retinal structure and function during sterile inflammation in diseases such as AMD.

Original languageEnglish
Article number1666
Pages (from-to)1-17
Number of pages17
JournalFrontiers in Immunology
Volume9
Issue numberJUL
DOIs
Publication statusPublished - 18 Jul 2018
Externally publishedYes

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