Dynamin 2 is essential for mammalian spermatogenesis

Kate A. Redgrove, Ilana R. Bernstein, Victoria J. Pye, Bettina P. Mihalas, Jessie M. Sutherland, Brett Nixon, Adam McCluskey, Phillip J. Robinson, Janet E. Holt, Eileen A. McLaughlin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The dynamin family of proteins play important regulatory roles in membrane remodelling and endocytosis, especially within brain and neuronal tissues. In the context of reproduction, dynamin 1 (DNM1) and dynamin 2 (DNM2) have recently been shown to act as key mediators of sperm acrosome formation and function. However, little is known about the roles that these proteins play in the developing testicular germ cells. In this study, we employed a DNM2 germ cell-specific knockout model to investigate the role of DNM2 in spermatogenesis. We demonstrate that ablation of DNM2 in early spermatogenesis results in germ cell arrest during prophase I of meiosis, subsequent loss of all post-meiotic germ cells and concomitant sterility. These effects become exacerbated with age, and ultimately result in the demise of the spermatogonial stem cells and a Sertoli cell only phenotype. We also demonstrate that DNM2 activity may be temporally regulated by phosphorylation of DNM2 via the kinase CDK1 in spermatogonia, and dephosphorylation by phosphatase PPP3CA during meiotic and post-meiotic spermatogenesis.

Original languageEnglish
Article number35084
Pages (from-to)1-14
Number of pages14
JournalScientific Reports
Volume6
Issue number1
DOIs
Publication statusPublished - 11 Oct 2016
Externally publishedYes

Fingerprint

Dynamin II
Spermatogenesis
Germ Cells
Dynamin I
Meiotic Prophase I
Dynamins
Acrosome
Spermatogonia
Sertoli Cells
Meiosis
Endocytosis
Phosphoric Monoester Hydrolases
Infertility
Reproduction
Spermatozoa
Proteins
Phosphotransferases
Stem Cells
Phosphorylation
Phenotype

Cite this

Redgrove, K. A., Bernstein, I. R., Pye, V. J., Mihalas, B. P., Sutherland, J. M., Nixon, B., ... McLaughlin, E. A. (2016). Dynamin 2 is essential for mammalian spermatogenesis. Scientific Reports, 6(1), 1-14. [35084]. https://doi.org/10.1038/srep35084
Redgrove, Kate A. ; Bernstein, Ilana R. ; Pye, Victoria J. ; Mihalas, Bettina P. ; Sutherland, Jessie M. ; Nixon, Brett ; McCluskey, Adam ; Robinson, Phillip J. ; Holt, Janet E. ; McLaughlin, Eileen A. / Dynamin 2 is essential for mammalian spermatogenesis. In: Scientific Reports. 2016 ; Vol. 6, No. 1. pp. 1-14.
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Redgrove, KA, Bernstein, IR, Pye, VJ, Mihalas, BP, Sutherland, JM, Nixon, B, McCluskey, A, Robinson, PJ, Holt, JE & McLaughlin, EA 2016, 'Dynamin 2 is essential for mammalian spermatogenesis', Scientific Reports, vol. 6, no. 1, 35084, pp. 1-14. https://doi.org/10.1038/srep35084

Dynamin 2 is essential for mammalian spermatogenesis. / Redgrove, Kate A.; Bernstein, Ilana R.; Pye, Victoria J.; Mihalas, Bettina P.; Sutherland, Jessie M.; Nixon, Brett; McCluskey, Adam; Robinson, Phillip J.; Holt, Janet E.; McLaughlin, Eileen A.

In: Scientific Reports, Vol. 6, No. 1, 35084, 11.10.2016, p. 1-14.

Research output: Contribution to journalArticle

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Redgrove KA, Bernstein IR, Pye VJ, Mihalas BP, Sutherland JM, Nixon B et al. Dynamin 2 is essential for mammalian spermatogenesis. Scientific Reports. 2016 Oct 11;6(1):1-14. 35084. https://doi.org/10.1038/srep35084