TY - JOUR
T1 - Early stages of xy sex chromosomes differentiation in the fish Hoplias malabaricus (Characiformes, erythrinidae) revealed by DNA repeats accumulation
AU - de Freitas, Natália Lourenço
AU - Al-Rikabi, Ahmed Basheer Hamid
AU - Bertollo, Luiz Antonio Carlos
AU - Ezaz, Tariq
AU - Yano, Cassia Fernanda
AU - de Oliveira, Ezequiel Aguiar
AU - Hatanaka, Terumi
AU - Cioffi, Marcelo de Bello
N1 - Funding Information:
This study was supported by Conselho Nacional de De-senvolvimento Científico e Tecnológico - CNPq (Proc. no 304992/2015-1 and 306896/2014-1) and Fundação de Amparo à Pesquisa do Estado de São Paulo-FAPESP (Proc. No 2014/22532-7). Natália Lourenço de Freitas is an undergraduate student in Biotechnology at Universidade Federal de São Carlos and sponsored by PIBIC/CNPq/UFSCar. TE is partially supported by an Australian Research Council (ARC) Future Fellowship (FT110100733).
Funding Information:
This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq (Proc. no 304992/2015-1 and 306896/2014-1) and Fundação de Amparo à Pesquisa do Estado de São Paulo- FAPESP (Proc. No 2014/22532-7). Natália Lourenço de Freitas is an undergraduate student in Biotechnology at Universidade Federal de São Carlos and sponsored by PIBIC/CNPq/UFSCar. TE is partially supported by an Australian Research Council (ARC) Future Fellowship (FT110100733).
Publisher Copyright:
© 2018 Bentham Science Publishers.
PY - 2018
Y1 - 2018
N2 - Background: Species with ‘young’ or nascent sex chromosomes provide unique opportunities to understand early evolutionary mechanisms (e.g. accumulation of repetitive sequences, cessation of recombination and gene loss) that drive the evolution of sex chromosomes. Among vertebrates, fishes exhibit highly diverse and a wide spectrum of sex-determining mechanisms and sex chromosomes, ranging from cryptic to highly differentiated ones, as well as, from simple to multiple sex chromosome systems. Such variability in sex chromosome morphology and composition not only exists within closely related taxa, but often within races/populations of the same species. Inside this context, the wolf fish Hoplias malabaricus offers opportunity to investigate the evolution of morphologically variable sex chromosomes within a species complex, as homomorphic to highly differentiated sex chromosome systems occur among its different karyomorphs. Materials & Methods: To discover various evolutionary stages of sex chromosomes and to compare their sequence composition among the wolf fish´s karyomorphs, we applied multipronged molecular cytogenetic approaches, including C-banding, repetitive DNAs mapping, Comparative Genomic Hybridization (CGH) and Whole Chromosomal Painting (WCP). Our study was able to characterize a cryptically differentiated XX/XY sex chromosome system in the karyomorph F of this species. Conclusion: The Y chromosome was clearly identified by an interstitial heterochromatic block on the short arms, primarily composed of microsatellite motifs and retrotransposons. Additionally, CGH also identified a male specific chromosome region in the same chromosomal location, implying that the accumulation of these repeats may have initiated the Y chromosome differentiation, as well as played a critical role towards the evolution and differentiation of sex chromosomes in various karyomorphs of this species.
AB - Background: Species with ‘young’ or nascent sex chromosomes provide unique opportunities to understand early evolutionary mechanisms (e.g. accumulation of repetitive sequences, cessation of recombination and gene loss) that drive the evolution of sex chromosomes. Among vertebrates, fishes exhibit highly diverse and a wide spectrum of sex-determining mechanisms and sex chromosomes, ranging from cryptic to highly differentiated ones, as well as, from simple to multiple sex chromosome systems. Such variability in sex chromosome morphology and composition not only exists within closely related taxa, but often within races/populations of the same species. Inside this context, the wolf fish Hoplias malabaricus offers opportunity to investigate the evolution of morphologically variable sex chromosomes within a species complex, as homomorphic to highly differentiated sex chromosome systems occur among its different karyomorphs. Materials & Methods: To discover various evolutionary stages of sex chromosomes and to compare their sequence composition among the wolf fish´s karyomorphs, we applied multipronged molecular cytogenetic approaches, including C-banding, repetitive DNAs mapping, Comparative Genomic Hybridization (CGH) and Whole Chromosomal Painting (WCP). Our study was able to characterize a cryptically differentiated XX/XY sex chromosome system in the karyomorph F of this species. Conclusion: The Y chromosome was clearly identified by an interstitial heterochromatic block on the short arms, primarily composed of microsatellite motifs and retrotransposons. Additionally, CGH also identified a male specific chromosome region in the same chromosomal location, implying that the accumulation of these repeats may have initiated the Y chromosome differentiation, as well as played a critical role towards the evolution and differentiation of sex chromosomes in various karyomorphs of this species.
KW - Comparative genomic hybridization
KW - Early XY differentiation
KW - Fish
KW - Repetitive DNAs
KW - Sex chromosomes
KW - Whole chromosome painting
UR - http://www.scopus.com/inward/record.url?scp=85043402722&partnerID=8YFLogxK
UR - http://www.mendeley.com/research/early-stages-xy-sex-chromosomes-differentiation-fish-hoplias-malabaricus-characiformes-erythrinidae
U2 - 10.2174/1389202918666170711160528
DO - 10.2174/1389202918666170711160528
M3 - Article
AN - SCOPUS:85043402722
SN - 1389-2029
VL - 19
SP - 216
EP - 226
JO - Current Genomics
JF - Current Genomics
IS - 3
ER -