Early stages of xy sex chromosomes differentiation in the fish Hoplias malabaricus (Characiformes, erythrinidae) revealed by DNA repeats accumulation

Natália Lourenço de Freitas, Ahmed Basheer Hamid Al-Rikabi, Luiz Antonio Carlos Bertollo, Tariq Ezaz, Cassia Fernanda Yano, Ezequiel Aguiar de Oliveira, Terumi Hatanaka, Marcelo de Bello Cioffi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Species with ‘young’ or nascent sex chromosomes provide unique opportunities to understand early evolutionary mechanisms (e.g. accumulation of repetitive sequences, cessation of recombination and gene loss) that drive the evolution of sex chromosomes. Among vertebrates, fishes exhibit highly diverse and a wide spectrum of sex-determining mechanisms and sex chromosomes, ranging from cryptic to highly differentiated ones, as well as, from simple to multiple sex chromosome systems. Such variability in sex chromosome morphology and composition not only exists within closely related taxa, but often within races/populations of the same species. Inside this context, the wolf fish Hoplias malabaricus offers opportunity to investigate the evolution of morphologically variable sex chromosomes within a species complex, as homomorphic to highly differentiated sex chromosome systems occur among its different karyomorphs. Materials & Methods: To discover various evolutionary stages of sex chromosomes and to compare their sequence composition among the wolf fish´s karyomorphs, we applied multipronged molecular cytogenetic approaches, including C-banding, repetitive DNAs mapping, Comparative Genomic Hybridization (CGH) and Whole Chromosomal Painting (WCP). Our study was able to characterize a cryptically differentiated XX/XY sex chromosome system in the karyomorph F of this species. Conclusion: The Y chromosome was clearly identified by an interstitial heterochromatic block on the short arms, primarily composed of microsatellite motifs and retrotransposons. Additionally, CGH also identified a male specific chromosome region in the same chromosomal location, implying that the accumulation of these repeats may have initiated the Y chromosome differentiation, as well as played a critical role towards the evolution and differentiation of sex chromosomes in various karyomorphs of this species.

Original languageEnglish
Pages (from-to)216-226
Number of pages11
JournalCurrent Genomics
Volume19
Issue number3
DOIs
Publication statusPublished - 1 Jan 2018

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Characiformes
Sex Differentiation
Sex Chromosomes
Fishes
DNA
Comparative Genomic Hybridization
Y Chromosome
Retroelements
Paintings
Nucleic Acid Repetitive Sequences
Cytogenetics
Microsatellite Repeats
Genetic Recombination
Vertebrates

Cite this

de Freitas, Natália Lourenço ; Al-Rikabi, Ahmed Basheer Hamid ; Bertollo, Luiz Antonio Carlos ; Ezaz, Tariq ; Yano, Cassia Fernanda ; de Oliveira, Ezequiel Aguiar ; Hatanaka, Terumi ; Cioffi, Marcelo de Bello. / Early stages of xy sex chromosomes differentiation in the fish Hoplias malabaricus (Characiformes, erythrinidae) revealed by DNA repeats accumulation. In: Current Genomics. 2018 ; Vol. 19, No. 3. pp. 216-226.
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abstract = "Background: Species with ‘young’ or nascent sex chromosomes provide unique opportunities to understand early evolutionary mechanisms (e.g. accumulation of repetitive sequences, cessation of recombination and gene loss) that drive the evolution of sex chromosomes. Among vertebrates, fishes exhibit highly diverse and a wide spectrum of sex-determining mechanisms and sex chromosomes, ranging from cryptic to highly differentiated ones, as well as, from simple to multiple sex chromosome systems. Such variability in sex chromosome morphology and composition not only exists within closely related taxa, but often within races/populations of the same species. Inside this context, the wolf fish Hoplias malabaricus offers opportunity to investigate the evolution of morphologically variable sex chromosomes within a species complex, as homomorphic to highly differentiated sex chromosome systems occur among its different karyomorphs. Materials & Methods: To discover various evolutionary stages of sex chromosomes and to compare their sequence composition among the wolf fish´s karyomorphs, we applied multipronged molecular cytogenetic approaches, including C-banding, repetitive DNAs mapping, Comparative Genomic Hybridization (CGH) and Whole Chromosomal Painting (WCP). Our study was able to characterize a cryptically differentiated XX/XY sex chromosome system in the karyomorph F of this species. Conclusion: The Y chromosome was clearly identified by an interstitial heterochromatic block on the short arms, primarily composed of microsatellite motifs and retrotransposons. Additionally, CGH also identified a male specific chromosome region in the same chromosomal location, implying that the accumulation of these repeats may have initiated the Y chromosome differentiation, as well as played a critical role towards the evolution and differentiation of sex chromosomes in various karyomorphs of this species.",
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Early stages of xy sex chromosomes differentiation in the fish Hoplias malabaricus (Characiformes, erythrinidae) revealed by DNA repeats accumulation. / de Freitas, Natália Lourenço; Al-Rikabi, Ahmed Basheer Hamid; Bertollo, Luiz Antonio Carlos; Ezaz, Tariq; Yano, Cassia Fernanda; de Oliveira, Ezequiel Aguiar; Hatanaka, Terumi; Cioffi, Marcelo de Bello.

In: Current Genomics, Vol. 19, No. 3, 01.01.2018, p. 216-226.

Research output: Contribution to journalArticle

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T1 - Early stages of xy sex chromosomes differentiation in the fish Hoplias malabaricus (Characiformes, erythrinidae) revealed by DNA repeats accumulation

AU - de Freitas, Natália Lourenço

AU - Al-Rikabi, Ahmed Basheer Hamid

AU - Bertollo, Luiz Antonio Carlos

AU - Ezaz, Tariq

AU - Yano, Cassia Fernanda

AU - de Oliveira, Ezequiel Aguiar

AU - Hatanaka, Terumi

AU - Cioffi, Marcelo de Bello

PY - 2018/1/1

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N2 - Background: Species with ‘young’ or nascent sex chromosomes provide unique opportunities to understand early evolutionary mechanisms (e.g. accumulation of repetitive sequences, cessation of recombination and gene loss) that drive the evolution of sex chromosomes. Among vertebrates, fishes exhibit highly diverse and a wide spectrum of sex-determining mechanisms and sex chromosomes, ranging from cryptic to highly differentiated ones, as well as, from simple to multiple sex chromosome systems. Such variability in sex chromosome morphology and composition not only exists within closely related taxa, but often within races/populations of the same species. Inside this context, the wolf fish Hoplias malabaricus offers opportunity to investigate the evolution of morphologically variable sex chromosomes within a species complex, as homomorphic to highly differentiated sex chromosome systems occur among its different karyomorphs. Materials & Methods: To discover various evolutionary stages of sex chromosomes and to compare their sequence composition among the wolf fish´s karyomorphs, we applied multipronged molecular cytogenetic approaches, including C-banding, repetitive DNAs mapping, Comparative Genomic Hybridization (CGH) and Whole Chromosomal Painting (WCP). Our study was able to characterize a cryptically differentiated XX/XY sex chromosome system in the karyomorph F of this species. Conclusion: The Y chromosome was clearly identified by an interstitial heterochromatic block on the short arms, primarily composed of microsatellite motifs and retrotransposons. Additionally, CGH also identified a male specific chromosome region in the same chromosomal location, implying that the accumulation of these repeats may have initiated the Y chromosome differentiation, as well as played a critical role towards the evolution and differentiation of sex chromosomes in various karyomorphs of this species.

AB - Background: Species with ‘young’ or nascent sex chromosomes provide unique opportunities to understand early evolutionary mechanisms (e.g. accumulation of repetitive sequences, cessation of recombination and gene loss) that drive the evolution of sex chromosomes. Among vertebrates, fishes exhibit highly diverse and a wide spectrum of sex-determining mechanisms and sex chromosomes, ranging from cryptic to highly differentiated ones, as well as, from simple to multiple sex chromosome systems. Such variability in sex chromosome morphology and composition not only exists within closely related taxa, but often within races/populations of the same species. Inside this context, the wolf fish Hoplias malabaricus offers opportunity to investigate the evolution of morphologically variable sex chromosomes within a species complex, as homomorphic to highly differentiated sex chromosome systems occur among its different karyomorphs. Materials & Methods: To discover various evolutionary stages of sex chromosomes and to compare their sequence composition among the wolf fish´s karyomorphs, we applied multipronged molecular cytogenetic approaches, including C-banding, repetitive DNAs mapping, Comparative Genomic Hybridization (CGH) and Whole Chromosomal Painting (WCP). Our study was able to characterize a cryptically differentiated XX/XY sex chromosome system in the karyomorph F of this species. Conclusion: The Y chromosome was clearly identified by an interstitial heterochromatic block on the short arms, primarily composed of microsatellite motifs and retrotransposons. Additionally, CGH also identified a male specific chromosome region in the same chromosomal location, implying that the accumulation of these repeats may have initiated the Y chromosome differentiation, as well as played a critical role towards the evolution and differentiation of sex chromosomes in various karyomorphs of this species.

KW - Comparative genomic hybridization

KW - Early XY differentiation

KW - Fish

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