Egr-1 mRNA expression is a marker for the direction of mammalian ocular growth

Regan ASHBY, Guang Zeng, Amelia Leotta, Dennis Tse, Sally McFadden

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    METHODS. To induce accelerated growth and myopia, guinea pigs wore a -5 diopter (D) lens over one eye from 4 to 11 days of age. To induce inhibited growth, the lens was removed after 7 days of -5 D lens wear, and the eye allowed to recover from myopia for 3 days. Ocular parameters and Egr-1 mRNA levels were subsequently assessed, and compared to untreated fellow eyes and eyes from untreated littermates. Possible circadian changes in Egr-1 mRNA levels were also determined in 18 additional animals by taking measures every 4 hours during a 24-hour cycle.

    PURPOSE. The immediate early gene Egr-1 is thought to form part of the pathway that mediates abnormal ocular growth. This study investigated whether the mRNA expression levels of Egr- 1 in a mammalian retina are modulated differentially, depending on the direction of ocular growth.

    RESULTS. Ocular compensation to a -5 D lens occurred after 7 days (D ∆ -4.8 D, D ∆ + 147 lm growth, N = 20). In 5 highly myopic eyes (∆ -7.4 D), Egr-1 mRNA levels in the retina were significantly downregulated relative to contralateral control (51%) and age-matched untreated (47%) eyes. Three days after the -5 D lens was removed, eyes had recovered from the myopia (∆ -0.5 D, relative change of + 2.9 D, N = 4) and Egr-1 mRNA levels were significantly elevated relative to contralateral (212%) and untreated (234%) eyes, respectively. Normal Egr- 1 mRNA expression was higher in the middle of the day than in the middle of the night. Immunolabeling showed strong Egr-1 reactivity in cell bodies in the inner nuclear and ganglion cell layers.

    CONCLUSIONS. Egr-1 mRNA levels in a mammalian retina show a bi-directional persistent response to opposing ocular growth stimuli. This suggests retinal Egr-1 might act as a signal for the direction of ocular growth in different species.

    Original languageEnglish
    Pages (from-to)5911-5921
    Number of pages11
    JournalInvestigative Ophthalmology and Visual Science
    Volume55
    Issue number9
    DOIs
    Publication statusPublished - 2014

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    Messenger RNA
    Growth
    Lenses
    Retina
    Myopia
    Direction compound
    Crystalline Lens
    Immediate-Early Genes
    Ganglia
    Guinea Pigs
    Down-Regulation

    Cite this

    ASHBY, Regan ; Zeng, Guang ; Leotta, Amelia ; Tse, Dennis ; McFadden, Sally. / Egr-1 mRNA expression is a marker for the direction of mammalian ocular growth. In: Investigative Ophthalmology and Visual Science. 2014 ; Vol. 55, No. 9. pp. 5911-5921.
    @article{3914099061d346c087cfc2a881614045,
    title = "Egr-1 mRNA expression is a marker for the direction of mammalian ocular growth",
    abstract = "METHODS. To induce accelerated growth and myopia, guinea pigs wore a -5 diopter (D) lens over one eye from 4 to 11 days of age. To induce inhibited growth, the lens was removed after 7 days of -5 D lens wear, and the eye allowed to recover from myopia for 3 days. Ocular parameters and Egr-1 mRNA levels were subsequently assessed, and compared to untreated fellow eyes and eyes from untreated littermates. Possible circadian changes in Egr-1 mRNA levels were also determined in 18 additional animals by taking measures every 4 hours during a 24-hour cycle.PURPOSE. The immediate early gene Egr-1 is thought to form part of the pathway that mediates abnormal ocular growth. This study investigated whether the mRNA expression levels of Egr- 1 in a mammalian retina are modulated differentially, depending on the direction of ocular growth.RESULTS. Ocular compensation to a -5 D lens occurred after 7 days (D ∆ -4.8 D, D ∆ + 147 lm growth, N = 20). In 5 highly myopic eyes (∆ -7.4 D), Egr-1 mRNA levels in the retina were significantly downregulated relative to contralateral control (51{\%}) and age-matched untreated (47{\%}) eyes. Three days after the -5 D lens was removed, eyes had recovered from the myopia (∆ -0.5 D, relative change of + 2.9 D, N = 4) and Egr-1 mRNA levels were significantly elevated relative to contralateral (212{\%}) and untreated (234{\%}) eyes, respectively. Normal Egr- 1 mRNA expression was higher in the middle of the day than in the middle of the night. Immunolabeling showed strong Egr-1 reactivity in cell bodies in the inner nuclear and ganglion cell layers.CONCLUSIONS. Egr-1 mRNA levels in a mammalian retina show a bi-directional persistent response to opposing ocular growth stimuli. This suggests retinal Egr-1 might act as a signal for the direction of ocular growth in different species.",
    keywords = "Visual-Neuroscience, Myopia, Gene-Expression, Guinea pig, Refractive error, Egr-1, Retina",
    author = "Regan ASHBY and Guang Zeng and Amelia Leotta and Dennis Tse and Sally McFadden",
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    Egr-1 mRNA expression is a marker for the direction of mammalian ocular growth. / ASHBY, Regan; Zeng, Guang; Leotta, Amelia; Tse, Dennis; McFadden, Sally.

    In: Investigative Ophthalmology and Visual Science, Vol. 55, No. 9, 2014, p. 5911-5921.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Egr-1 mRNA expression is a marker for the direction of mammalian ocular growth

    AU - ASHBY, Regan

    AU - Zeng, Guang

    AU - Leotta, Amelia

    AU - Tse, Dennis

    AU - McFadden, Sally

    PY - 2014

    Y1 - 2014

    N2 - METHODS. To induce accelerated growth and myopia, guinea pigs wore a -5 diopter (D) lens over one eye from 4 to 11 days of age. To induce inhibited growth, the lens was removed after 7 days of -5 D lens wear, and the eye allowed to recover from myopia for 3 days. Ocular parameters and Egr-1 mRNA levels were subsequently assessed, and compared to untreated fellow eyes and eyes from untreated littermates. Possible circadian changes in Egr-1 mRNA levels were also determined in 18 additional animals by taking measures every 4 hours during a 24-hour cycle.PURPOSE. The immediate early gene Egr-1 is thought to form part of the pathway that mediates abnormal ocular growth. This study investigated whether the mRNA expression levels of Egr- 1 in a mammalian retina are modulated differentially, depending on the direction of ocular growth.RESULTS. Ocular compensation to a -5 D lens occurred after 7 days (D ∆ -4.8 D, D ∆ + 147 lm growth, N = 20). In 5 highly myopic eyes (∆ -7.4 D), Egr-1 mRNA levels in the retina were significantly downregulated relative to contralateral control (51%) and age-matched untreated (47%) eyes. Three days after the -5 D lens was removed, eyes had recovered from the myopia (∆ -0.5 D, relative change of + 2.9 D, N = 4) and Egr-1 mRNA levels were significantly elevated relative to contralateral (212%) and untreated (234%) eyes, respectively. Normal Egr- 1 mRNA expression was higher in the middle of the day than in the middle of the night. Immunolabeling showed strong Egr-1 reactivity in cell bodies in the inner nuclear and ganglion cell layers.CONCLUSIONS. Egr-1 mRNA levels in a mammalian retina show a bi-directional persistent response to opposing ocular growth stimuli. This suggests retinal Egr-1 might act as a signal for the direction of ocular growth in different species.

    AB - METHODS. To induce accelerated growth and myopia, guinea pigs wore a -5 diopter (D) lens over one eye from 4 to 11 days of age. To induce inhibited growth, the lens was removed after 7 days of -5 D lens wear, and the eye allowed to recover from myopia for 3 days. Ocular parameters and Egr-1 mRNA levels were subsequently assessed, and compared to untreated fellow eyes and eyes from untreated littermates. Possible circadian changes in Egr-1 mRNA levels were also determined in 18 additional animals by taking measures every 4 hours during a 24-hour cycle.PURPOSE. The immediate early gene Egr-1 is thought to form part of the pathway that mediates abnormal ocular growth. This study investigated whether the mRNA expression levels of Egr- 1 in a mammalian retina are modulated differentially, depending on the direction of ocular growth.RESULTS. Ocular compensation to a -5 D lens occurred after 7 days (D ∆ -4.8 D, D ∆ + 147 lm growth, N = 20). In 5 highly myopic eyes (∆ -7.4 D), Egr-1 mRNA levels in the retina were significantly downregulated relative to contralateral control (51%) and age-matched untreated (47%) eyes. Three days after the -5 D lens was removed, eyes had recovered from the myopia (∆ -0.5 D, relative change of + 2.9 D, N = 4) and Egr-1 mRNA levels were significantly elevated relative to contralateral (212%) and untreated (234%) eyes, respectively. Normal Egr- 1 mRNA expression was higher in the middle of the day than in the middle of the night. Immunolabeling showed strong Egr-1 reactivity in cell bodies in the inner nuclear and ganglion cell layers.CONCLUSIONS. Egr-1 mRNA levels in a mammalian retina show a bi-directional persistent response to opposing ocular growth stimuli. This suggests retinal Egr-1 might act as a signal for the direction of ocular growth in different species.

    KW - Visual-Neuroscience

    KW - Myopia

    KW - Gene-Expression

    KW - Guinea pig

    KW - Refractive error

    KW - Egr-1

    KW - Retina

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    UR - http://www.mendeley.com/research/egr1-mrna-expression-marker-direction-mammalian-ocular-growth

    U2 - 10.1167/iovs.13-11708

    DO - 10.1167/iovs.13-11708

    M3 - Article

    VL - 55

    SP - 5911

    EP - 5921

    JO - Investigative Ophthalmology

    JF - Investigative Ophthalmology

    SN - 0146-0404

    IS - 9

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