Elevated plasma glutathione peroxidase concentration in acute severe asthma

comparison with plasma glutathione peroxidase activity, selenium and malondialdehyde

Glenn A Jacobson, K C Yee, C H Ng

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

OBJECTIVE: To investigate plasma glutathione peroxidase concentration, glutathione peroxidase activity, plasma selenium and oxidative stress in acute severe asthma. MATERIAL AND METHODS: The study was case-control in design, with cases presenting to the emergency department with acute severe asthma and controls randomly selected from a larger cross-sectional study. Plasma malondialdehyde (MDA) was used as a measure of oxidative stress and plasma selenium was measured using ICP-MS. Glutathione peroxidase (GPx) activity was analysed using a colorimetric GPx assay and plasma GPx level was measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: Fifteen cases [mean (range) predicted peak expiratory flow rate (PEFR) of 43% (20-69)] and 15 matched controls were recruited. MDA levels (mean+/-SD) were higher in acute asthma subjects (1.30+/-0.56 micromol/L) than in controls (0.86+/-0.53 micromol/L; p<0.05). There were no differences between cases and controls for selenium (99+/-34 microg/L versus 109+/-17 microg/L) or for GPx activity (39+/-25 nmol min(-1) mL(-1) versus 38+/-24 nmol min(-1) mL(-1)), however, GPx plasma levels measured by ELISA were higher in cases than controls (22.5+/-10.8 mg/L versus 13.8+/-7.3 mg/L; p<0.05). CONCLUSIONS: Patients with acute severe asthma demonstrated increased MDA levels but no differences in plasma selenium levels or GPx activity. GPx levels measured by ELISA were elevated in severe asthma. These results are consistent with an adaptive up-regulation of GPx to protect against oxidative stress.

Original languageEnglish
Pages (from-to)423-30
Number of pages8
JournalScandinavian Journal of Clinical and Laboratory Investigation
Volume67
Issue number4
DOIs
Publication statusPublished - 2007
Externally publishedYes

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Selenium
Glutathione Peroxidase
Malondialdehyde
Thermodynamic properties
Asthma
Plasmas
Immunosorbents
Oxidative stress
Assays
Oxidative Stress
Enzyme-Linked Immunosorbent Assay
Enzymes
Peak Expiratory Flow Rate
Inductively coupled plasma
Case-Control Studies
Hospital Emergency Service
Up-Regulation
Cross-Sectional Studies
Flow rate

Cite this

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title = "Elevated plasma glutathione peroxidase concentration in acute severe asthma: comparison with plasma glutathione peroxidase activity, selenium and malondialdehyde",
abstract = "OBJECTIVE: To investigate plasma glutathione peroxidase concentration, glutathione peroxidase activity, plasma selenium and oxidative stress in acute severe asthma. MATERIAL AND METHODS: The study was case-control in design, with cases presenting to the emergency department with acute severe asthma and controls randomly selected from a larger cross-sectional study. Plasma malondialdehyde (MDA) was used as a measure of oxidative stress and plasma selenium was measured using ICP-MS. Glutathione peroxidase (GPx) activity was analysed using a colorimetric GPx assay and plasma GPx level was measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: Fifteen cases [mean (range) predicted peak expiratory flow rate (PEFR) of 43{\%} (20-69)] and 15 matched controls were recruited. MDA levels (mean+/-SD) were higher in acute asthma subjects (1.30+/-0.56 micromol/L) than in controls (0.86+/-0.53 micromol/L; p<0.05). There were no differences between cases and controls for selenium (99+/-34 microg/L versus 109+/-17 microg/L) or for GPx activity (39+/-25 nmol min(-1) mL(-1) versus 38+/-24 nmol min(-1) mL(-1)), however, GPx plasma levels measured by ELISA were higher in cases than controls (22.5+/-10.8 mg/L versus 13.8+/-7.3 mg/L; p<0.05). CONCLUSIONS: Patients with acute severe asthma demonstrated increased MDA levels but no differences in plasma selenium levels or GPx activity. GPx levels measured by ELISA were elevated in severe asthma. These results are consistent with an adaptive up-regulation of GPx to protect against oxidative stress.",
keywords = "Adolescent, Adult, Aged, Asthma, Case-Control Studies, Chromatography, High Pressure Liquid, Female, Glutathione Peroxidase, Humans, Male, Malondialdehyde, Mass Spectrometry, Middle Aged, Oxidative Stress, Reference Values, Selenium, Status Asthmaticus, Up-Regulation, Comparative Study, Journal Article",
author = "Jacobson, {Glenn A} and Yee, {K C} and Ng, {C H}",
year = "2007",
doi = "10.1080/00365510601153353",
language = "English",
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pages = "423--30",
journal = "Scandinavian Journal of Clinical and Laboratory Investigation",
issn = "0036-5513",
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}

TY - JOUR

T1 - Elevated plasma glutathione peroxidase concentration in acute severe asthma

T2 - comparison with plasma glutathione peroxidase activity, selenium and malondialdehyde

AU - Jacobson, Glenn A

AU - Yee, K C

AU - Ng, C H

PY - 2007

Y1 - 2007

N2 - OBJECTIVE: To investigate plasma glutathione peroxidase concentration, glutathione peroxidase activity, plasma selenium and oxidative stress in acute severe asthma. MATERIAL AND METHODS: The study was case-control in design, with cases presenting to the emergency department with acute severe asthma and controls randomly selected from a larger cross-sectional study. Plasma malondialdehyde (MDA) was used as a measure of oxidative stress and plasma selenium was measured using ICP-MS. Glutathione peroxidase (GPx) activity was analysed using a colorimetric GPx assay and plasma GPx level was measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: Fifteen cases [mean (range) predicted peak expiratory flow rate (PEFR) of 43% (20-69)] and 15 matched controls were recruited. MDA levels (mean+/-SD) were higher in acute asthma subjects (1.30+/-0.56 micromol/L) than in controls (0.86+/-0.53 micromol/L; p<0.05). There were no differences between cases and controls for selenium (99+/-34 microg/L versus 109+/-17 microg/L) or for GPx activity (39+/-25 nmol min(-1) mL(-1) versus 38+/-24 nmol min(-1) mL(-1)), however, GPx plasma levels measured by ELISA were higher in cases than controls (22.5+/-10.8 mg/L versus 13.8+/-7.3 mg/L; p<0.05). CONCLUSIONS: Patients with acute severe asthma demonstrated increased MDA levels but no differences in plasma selenium levels or GPx activity. GPx levels measured by ELISA were elevated in severe asthma. These results are consistent with an adaptive up-regulation of GPx to protect against oxidative stress.

AB - OBJECTIVE: To investigate plasma glutathione peroxidase concentration, glutathione peroxidase activity, plasma selenium and oxidative stress in acute severe asthma. MATERIAL AND METHODS: The study was case-control in design, with cases presenting to the emergency department with acute severe asthma and controls randomly selected from a larger cross-sectional study. Plasma malondialdehyde (MDA) was used as a measure of oxidative stress and plasma selenium was measured using ICP-MS. Glutathione peroxidase (GPx) activity was analysed using a colorimetric GPx assay and plasma GPx level was measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: Fifteen cases [mean (range) predicted peak expiratory flow rate (PEFR) of 43% (20-69)] and 15 matched controls were recruited. MDA levels (mean+/-SD) were higher in acute asthma subjects (1.30+/-0.56 micromol/L) than in controls (0.86+/-0.53 micromol/L; p<0.05). There were no differences between cases and controls for selenium (99+/-34 microg/L versus 109+/-17 microg/L) or for GPx activity (39+/-25 nmol min(-1) mL(-1) versus 38+/-24 nmol min(-1) mL(-1)), however, GPx plasma levels measured by ELISA were higher in cases than controls (22.5+/-10.8 mg/L versus 13.8+/-7.3 mg/L; p<0.05). CONCLUSIONS: Patients with acute severe asthma demonstrated increased MDA levels but no differences in plasma selenium levels or GPx activity. GPx levels measured by ELISA were elevated in severe asthma. These results are consistent with an adaptive up-regulation of GPx to protect against oxidative stress.

KW - Adolescent

KW - Adult

KW - Aged

KW - Asthma

KW - Case-Control Studies

KW - Chromatography, High Pressure Liquid

KW - Female

KW - Glutathione Peroxidase

KW - Humans

KW - Male

KW - Malondialdehyde

KW - Mass Spectrometry

KW - Middle Aged

KW - Oxidative Stress

KW - Reference Values

KW - Selenium

KW - Status Asthmaticus

KW - Up-Regulation

KW - Comparative Study

KW - Journal Article

U2 - 10.1080/00365510601153353

DO - 10.1080/00365510601153353

M3 - Article

VL - 67

SP - 423

EP - 430

JO - Scandinavian Journal of Clinical and Laboratory Investigation

JF - Scandinavian Journal of Clinical and Laboratory Investigation

SN - 0036-5513

IS - 4

ER -