Emerging patterns of genome organization in Notopteridae species (Teleostei, Osteoglossiformes) as revealed by Zoo-FISH and Comparative Genomic Hybridization (CGH)

Felipe Faix Barby, L Bertollo, Ezequiel Aguiar De Oliveira, Cassia Fernanda Yano, Terumi Hatanaka, Petr Ráb, A Sember, Tariq EZAZ, Roberto Ferreira Artoni, Thomas Liehr, Ahmed Basheer Hamid Al-Rikabi, V Trifonov, Edivaldo H. C. de Oliveira, Wagner Franco Molina, Oladele Ilesanmi Jegede, Alongklod Tanomtong, Marcelo de Bello Cioffi

Research output: Contribution to journalArticle

1 Citation (Scopus)
1 Downloads (Pure)

Abstract

Notopteridae (Teleostei, Osteoglossiformes) represents an old fish lineage with ten currently recognized species distributed in African and Southeastern Asian rivers. Their karyotype structures and diploid numbers remained conserved over long evolutionary periods, since African and Asian lineages diverged approximately 120 Mya. However, a significant genetic diversity was already identified for these species using molecular data. Thus, why the evolutionary relationships within Notopteridae are so diverse at the genomic level but so conserved in terms of their karyotypes? In an attempt to develop a more comprehensive picture of the karyotype and genome evolution in Notopteridae, we performed comparative genomic hybridization (CGH) and cross-species (Zoo-FISH) whole chromosome painting experiments to explore chromosome-scale intergenomic divergence among seven notopterid species, collected in different African and Southeast Asian river basins. CGH demonstrated an advanced stage of sequence divergence among the species and Zoo-FISH experiments showed diffuse and limited homology on inter-generic level, showing a temporal reduction of evolutionarily conserved syntenic regions. The sharing of a conserved chromosomal region revealed by Zoo-FISH in these species provides perspectives that several other homologous syntenic regions have remained conserved among their genomes despite long temporal isolation. In summary, Notopteridae is an interesting model for tracking the chromosome evolution as it is (i) ancestral vertebrate group with Gondwanan distribution and (ii) an example of animal group exhibiting karyotype stasis. The present study brings new insights into degree of genome divergence vs. conservation at chromosomal and sub-chromosomal level in representative sampling of this group.

Original languageEnglish
Article number1112
Pages (from-to)1-11
Number of pages11
JournalScientific Reports
Volume9
DOIs
Publication statusPublished - 2019

Fingerprint

Osteoglossiformes
comparative genomic hybridization
zoos
karyotyping
genome
chromosomes
diploidy
vertebrates
genomics
genetic variation
rivers
Notopteridae
fish
animals

Cite this

Barby, Felipe Faix ; Bertollo, L ; De Oliveira, Ezequiel Aguiar ; Yano, Cassia Fernanda ; Hatanaka, Terumi ; Ráb, Petr ; Sember, A ; EZAZ, Tariq ; Artoni, Roberto Ferreira ; Liehr, Thomas ; Al-Rikabi, Ahmed Basheer Hamid ; Trifonov, V ; de Oliveira, Edivaldo H. C. ; Franco Molina, Wagner ; Jegede, Oladele Ilesanmi ; Tanomtong, Alongklod ; Cioffi, Marcelo de Bello . / Emerging patterns of genome organization in Notopteridae species (Teleostei, Osteoglossiformes) as revealed by Zoo-FISH and Comparative Genomic Hybridization (CGH). In: Scientific Reports. 2019 ; Vol. 9. pp. 1-11.
@article{2f965f6557444e7193b055eefb3f5818,
title = "Emerging patterns of genome organization in Notopteridae species (Teleostei, Osteoglossiformes) as revealed by Zoo-FISH and Comparative Genomic Hybridization (CGH)",
abstract = "Notopteridae (Teleostei, Osteoglossiformes) represents an old fish lineage with ten currently recognized species distributed in African and Southeastern Asian rivers. Their karyotype structures and diploid numbers remained conserved over long evolutionary periods, since African and Asian lineages diverged approximately 120 Mya. However, a significant genetic diversity was already identified for these species using molecular data. Thus, why the evolutionary relationships within Notopteridae are so diverse at the genomic level but so conserved in terms of their karyotypes? In an attempt to develop a more comprehensive picture of the karyotype and genome evolution in Notopteridae, we performed comparative genomic hybridization (CGH) and cross-species (Zoo-FISH) whole chromosome painting experiments to explore chromosome-scale intergenomic divergence among seven notopterid species, collected in different African and Southeast Asian river basins. CGH demonstrated an advanced stage of sequence divergence among the species and Zoo-FISH experiments showed diffuse and limited homology on inter-generic level, showing a temporal reduction of evolutionarily conserved syntenic regions. The sharing of a conserved chromosomal region revealed by Zoo-FISH in these species provides perspectives that several other homologous syntenic regions have remained conserved among their genomes despite long temporal isolation. In summary, Notopteridae is an interesting model for tracking the chromosome evolution as it is (i) ancestral vertebrate group with Gondwanan distribution and (ii) an example of animal group exhibiting karyotype stasis. The present study brings new insights into degree of genome divergence vs. conservation at chromosomal and sub-chromosomal level in representative sampling of this group.",
author = "Barby, {Felipe Faix} and L Bertollo and {De Oliveira}, {Ezequiel Aguiar} and Yano, {Cassia Fernanda} and Terumi Hatanaka and Petr R{\'a}b and A Sember and Tariq EZAZ and Artoni, {Roberto Ferreira} and Thomas Liehr and Al-Rikabi, {Ahmed Basheer Hamid} and V Trifonov and {de Oliveira}, {Edivaldo H. C.} and {Franco Molina}, Wagner and Jegede, {Oladele Ilesanmi} and Alongklod Tanomtong and Cioffi, {Marcelo de Bello}",
year = "2019",
doi = "10.1038/s41598-019-38617-4",
language = "English",
volume = "9",
pages = "1--11",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

Barby, FF, Bertollo, L, De Oliveira, EA, Yano, CF, Hatanaka, T, Ráb, P, Sember, A, EZAZ, T, Artoni, RF, Liehr, T, Al-Rikabi, ABH, Trifonov, V, de Oliveira, EHC, Franco Molina, W, Jegede, OI, Tanomtong, A & Cioffi, MDB 2019, 'Emerging patterns of genome organization in Notopteridae species (Teleostei, Osteoglossiformes) as revealed by Zoo-FISH and Comparative Genomic Hybridization (CGH)', Scientific Reports, vol. 9, 1112, pp. 1-11. https://doi.org/10.1038/s41598-019-38617-4

Emerging patterns of genome organization in Notopteridae species (Teleostei, Osteoglossiformes) as revealed by Zoo-FISH and Comparative Genomic Hybridization (CGH). / Barby, Felipe Faix; Bertollo, L; De Oliveira, Ezequiel Aguiar; Yano, Cassia Fernanda; Hatanaka, Terumi; Ráb, Petr; Sember, A; EZAZ, Tariq; Artoni, Roberto Ferreira; Liehr, Thomas; Al-Rikabi, Ahmed Basheer Hamid; Trifonov, V; de Oliveira, Edivaldo H. C. ; Franco Molina, Wagner ; Jegede, Oladele Ilesanmi; Tanomtong, Alongklod; Cioffi, Marcelo de Bello .

In: Scientific Reports, Vol. 9, 1112, 2019, p. 1-11.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Emerging patterns of genome organization in Notopteridae species (Teleostei, Osteoglossiformes) as revealed by Zoo-FISH and Comparative Genomic Hybridization (CGH)

AU - Barby, Felipe Faix

AU - Bertollo, L

AU - De Oliveira, Ezequiel Aguiar

AU - Yano, Cassia Fernanda

AU - Hatanaka, Terumi

AU - Ráb, Petr

AU - Sember, A

AU - EZAZ, Tariq

AU - Artoni, Roberto Ferreira

AU - Liehr, Thomas

AU - Al-Rikabi, Ahmed Basheer Hamid

AU - Trifonov, V

AU - de Oliveira, Edivaldo H. C.

AU - Franco Molina, Wagner

AU - Jegede, Oladele Ilesanmi

AU - Tanomtong, Alongklod

AU - Cioffi, Marcelo de Bello

PY - 2019

Y1 - 2019

N2 - Notopteridae (Teleostei, Osteoglossiformes) represents an old fish lineage with ten currently recognized species distributed in African and Southeastern Asian rivers. Their karyotype structures and diploid numbers remained conserved over long evolutionary periods, since African and Asian lineages diverged approximately 120 Mya. However, a significant genetic diversity was already identified for these species using molecular data. Thus, why the evolutionary relationships within Notopteridae are so diverse at the genomic level but so conserved in terms of their karyotypes? In an attempt to develop a more comprehensive picture of the karyotype and genome evolution in Notopteridae, we performed comparative genomic hybridization (CGH) and cross-species (Zoo-FISH) whole chromosome painting experiments to explore chromosome-scale intergenomic divergence among seven notopterid species, collected in different African and Southeast Asian river basins. CGH demonstrated an advanced stage of sequence divergence among the species and Zoo-FISH experiments showed diffuse and limited homology on inter-generic level, showing a temporal reduction of evolutionarily conserved syntenic regions. The sharing of a conserved chromosomal region revealed by Zoo-FISH in these species provides perspectives that several other homologous syntenic regions have remained conserved among their genomes despite long temporal isolation. In summary, Notopteridae is an interesting model for tracking the chromosome evolution as it is (i) ancestral vertebrate group with Gondwanan distribution and (ii) an example of animal group exhibiting karyotype stasis. The present study brings new insights into degree of genome divergence vs. conservation at chromosomal and sub-chromosomal level in representative sampling of this group.

AB - Notopteridae (Teleostei, Osteoglossiformes) represents an old fish lineage with ten currently recognized species distributed in African and Southeastern Asian rivers. Their karyotype structures and diploid numbers remained conserved over long evolutionary periods, since African and Asian lineages diverged approximately 120 Mya. However, a significant genetic diversity was already identified for these species using molecular data. Thus, why the evolutionary relationships within Notopteridae are so diverse at the genomic level but so conserved in terms of their karyotypes? In an attempt to develop a more comprehensive picture of the karyotype and genome evolution in Notopteridae, we performed comparative genomic hybridization (CGH) and cross-species (Zoo-FISH) whole chromosome painting experiments to explore chromosome-scale intergenomic divergence among seven notopterid species, collected in different African and Southeast Asian river basins. CGH demonstrated an advanced stage of sequence divergence among the species and Zoo-FISH experiments showed diffuse and limited homology on inter-generic level, showing a temporal reduction of evolutionarily conserved syntenic regions. The sharing of a conserved chromosomal region revealed by Zoo-FISH in these species provides perspectives that several other homologous syntenic regions have remained conserved among their genomes despite long temporal isolation. In summary, Notopteridae is an interesting model for tracking the chromosome evolution as it is (i) ancestral vertebrate group with Gondwanan distribution and (ii) an example of animal group exhibiting karyotype stasis. The present study brings new insights into degree of genome divergence vs. conservation at chromosomal and sub-chromosomal level in representative sampling of this group.

UR - http://www.scopus.com/inward/record.url?scp=85061030740&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/emerging-patterns-genome-organization-notopteridae-species-teleostei-osteoglossiformes-revealed-zoof

U2 - 10.1038/s41598-019-38617-4

DO - 10.1038/s41598-019-38617-4

M3 - Article

VL - 9

SP - 1

EP - 11

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 1112

ER -