Enhanced virus resistance of transgenic mice expressing the human MxA protein

J. Pavlovic, H. A. Arzet, H. P. Hefti, M. Frese, D. Rost, B. Ernst, E. Kolb, P. Staeheli, O. Haller

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

MxA is a GTPase that accumulates to high levels in the cytoplasm of interferon-treated human cells. Expression of MxA cDNA confers to transfected cell lines a high degree of resistance against several RNA viruses, including influenza, measles, vesicular stomatitis, and Thogoto viruses. We have now generated transgenic mice that express MxA cDNA in the brain and other organs under the control of a constitutive promoter. Embryonic fibroblasts derived from the transgenic mice were nonpermissive for Thogoto virus and showed reduced susceptibility for influenza A and vesicular stomatitis viruses. The transgenic animals survived challenges with high doses of Thogoto virus by the intracerebral or intraperitoneal route. Furthermore, the transgenic mice were more resistant than their nontransgenic littermates to intracerebral infections with influenza A and vesicular stomatitis viruses. These results demonstrate that MxA is a powerful antiviral agent in vivo, indicating that it may protect humans from the deleterious effects of infections with certain viral pathogens.

Original languageEnglish
Pages (from-to)4506-4510
Number of pages5
JournalJournal of Virology
Volume69
Issue number7
Publication statusPublished - 1995
Externally publishedYes

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Thogoto virus
Thogotovirus
Vesiculovirus
Vesicular Stomatitis
influenza
Human Influenza
Transgenic Mice
genetically modified organisms
Viruses
viruses
mice
Complementary DNA
antiviral agents
transgenic animals
Genetically Modified Animals
proteins
RNA Viruses
GTP Phosphohydrolases
guanosinetriphosphatase
Measles

Cite this

Pavlovic, J., Arzet, H. A., Hefti, H. P., Frese, M., Rost, D., Ernst, B., ... Haller, O. (1995). Enhanced virus resistance of transgenic mice expressing the human MxA protein. Journal of Virology, 69(7), 4506-4510.
Pavlovic, J. ; Arzet, H. A. ; Hefti, H. P. ; Frese, M. ; Rost, D. ; Ernst, B. ; Kolb, E. ; Staeheli, P. ; Haller, O. / Enhanced virus resistance of transgenic mice expressing the human MxA protein. In: Journal of Virology. 1995 ; Vol. 69, No. 7. pp. 4506-4510.
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Pavlovic, J, Arzet, HA, Hefti, HP, Frese, M, Rost, D, Ernst, B, Kolb, E, Staeheli, P & Haller, O 1995, 'Enhanced virus resistance of transgenic mice expressing the human MxA protein', Journal of Virology, vol. 69, no. 7, pp. 4506-4510.

Enhanced virus resistance of transgenic mice expressing the human MxA protein. / Pavlovic, J.; Arzet, H. A.; Hefti, H. P.; Frese, M.; Rost, D.; Ernst, B.; Kolb, E.; Staeheli, P.; Haller, O.

In: Journal of Virology, Vol. 69, No. 7, 1995, p. 4506-4510.

Research output: Contribution to journalArticle

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Pavlovic J, Arzet HA, Hefti HP, Frese M, Rost D, Ernst B et al. Enhanced virus resistance of transgenic mice expressing the human MxA protein. Journal of Virology. 1995;69(7):4506-4510.