Enhancement of fingermarks and visualizing DNA

P. Kanokwongnuwut, K. P. Kirkbride, H. Kobus, A. Linacre

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A novel method for detection and visualization of latent DNA using Diamond™ Nucleic Acid Dye (DD)staining has been developed. Applying DD to an object has the real potential to visualize DNA on a substrate from which a DNA profile can be generated, but it is important to determine whether this staining will adversely affect other forensic investigational techniques and vice versa. The aim of this study was to examine the interactions between staining a fingermark to detect DNA and then generate a DNA profile in combination with several standard latent fingermark enhancement methods. Six common fingerprint enhancements processes were chosen; (1)black powder, (2)black magnetic powder, (3)red magnetic powder, (4)white powder, (5)aluminum powder and (6)cyanoacrylate fuming. For all six methods, mark enhancement was carried out before DD staining and vice versa. DD is effective in detection of DNA in the presence of both aluminum and white finger mark powders and DD does not compromise the subsequent detection of ridge patterns if DD is applied first. Whilst magnetic powders could be used to successfully enhance latent fingermarks even after DD had been applied to them, latent DNA could not be observed in the marks irrespective of whether magnetic powder was applied before or after DD treatment. Magnetic powders did not adversely affect the profiling of DNA present in the marks. The application of DD to fingermarks did not adversely affect the enhancement of fingermarks using cyanoacrylate fuming. Whilst fluorescent particles resembling cells stained with DD were observed in marks either post-treated or pre-treated with cyanoacrylate vapor, DNA amplification and profiling was not successful. While it may be important in particular investigations to collect DNA profiles from latent fingermarks with continuous ridges and clear minutiae, the main utility of the technique described here would be in relation to investigations where enhancement has resulted in only partial or smudged marks. The results presented here indicate that if it is desirable to visualize latent DNA on an object but it is also planned to treat the object with cyanoacrylate vapor or magnetic powders then it is important to apply DD first, record the location of DNA and then apply the mark enhancement technique. For aluminum and white powder mark treatments such precautions are not important.

Original languageEnglish
Pages (from-to)99-105
Number of pages7
JournalForensic Science International
Volume300
DOIs
Publication statusPublished - Jul 2019

Fingerprint

Powders
DNA
Cyanoacrylates
Aluminum
Staining and Labeling
DNA Fingerprinting
Diamond
Dermatoglyphics
Nucleic Acids
Fingers
Coloring Agents

Cite this

Kanokwongnuwut, P., Kirkbride, K. P., Kobus, H., & Linacre, A. (2019). Enhancement of fingermarks and visualizing DNA. Forensic Science International, 300, 99-105. https://doi.org/10.1016/j.forsciint.2019.04.035
Kanokwongnuwut, P. ; Kirkbride, K. P. ; Kobus, H. ; Linacre, A. / Enhancement of fingermarks and visualizing DNA. In: Forensic Science International. 2019 ; Vol. 300. pp. 99-105.
@article{4fcf4b7d6459478587acda7bca11efb7,
title = "Enhancement of fingermarks and visualizing DNA",
abstract = "A novel method for detection and visualization of latent DNA using Diamond™ Nucleic Acid Dye (DD)staining has been developed. Applying DD to an object has the real potential to visualize DNA on a substrate from which a DNA profile can be generated, but it is important to determine whether this staining will adversely affect other forensic investigational techniques and vice versa. The aim of this study was to examine the interactions between staining a fingermark to detect DNA and then generate a DNA profile in combination with several standard latent fingermark enhancement methods. Six common fingerprint enhancements processes were chosen; (1)black powder, (2)black magnetic powder, (3)red magnetic powder, (4)white powder, (5)aluminum powder and (6)cyanoacrylate fuming. For all six methods, mark enhancement was carried out before DD staining and vice versa. DD is effective in detection of DNA in the presence of both aluminum and white finger mark powders and DD does not compromise the subsequent detection of ridge patterns if DD is applied first. Whilst magnetic powders could be used to successfully enhance latent fingermarks even after DD had been applied to them, latent DNA could not be observed in the marks irrespective of whether magnetic powder was applied before or after DD treatment. Magnetic powders did not adversely affect the profiling of DNA present in the marks. The application of DD to fingermarks did not adversely affect the enhancement of fingermarks using cyanoacrylate fuming. Whilst fluorescent particles resembling cells stained with DD were observed in marks either post-treated or pre-treated with cyanoacrylate vapor, DNA amplification and profiling was not successful. While it may be important in particular investigations to collect DNA profiles from latent fingermarks with continuous ridges and clear minutiae, the main utility of the technique described here would be in relation to investigations where enhancement has resulted in only partial or smudged marks. The results presented here indicate that if it is desirable to visualize latent DNA on an object but it is also planned to treat the object with cyanoacrylate vapor or magnetic powders then it is important to apply DD first, record the location of DNA and then apply the mark enhancement technique. For aluminum and white powder mark treatments such precautions are not important.",
keywords = "Diamond™ nucleic acid dye, Fingermark detection, Fingermark identification, Fingerprint powder, Stained cellular material",
author = "P. Kanokwongnuwut and Kirkbride, {K. P.} and H. Kobus and A. Linacre",
year = "2019",
month = "7",
doi = "10.1016/j.forsciint.2019.04.035",
language = "English",
volume = "300",
pages = "99--105",
journal = "Forensic Science",
issn = "0379-0738",
publisher = "Elsevier Ireland Ltd",

}

Kanokwongnuwut, P, Kirkbride, KP, Kobus, H & Linacre, A 2019, 'Enhancement of fingermarks and visualizing DNA', Forensic Science International, vol. 300, pp. 99-105. https://doi.org/10.1016/j.forsciint.2019.04.035

Enhancement of fingermarks and visualizing DNA. / Kanokwongnuwut, P.; Kirkbride, K. P.; Kobus, H.; Linacre, A.

In: Forensic Science International, Vol. 300, 07.2019, p. 99-105.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Enhancement of fingermarks and visualizing DNA

AU - Kanokwongnuwut, P.

AU - Kirkbride, K. P.

AU - Kobus, H.

AU - Linacre, A.

PY - 2019/7

Y1 - 2019/7

N2 - A novel method for detection and visualization of latent DNA using Diamond™ Nucleic Acid Dye (DD)staining has been developed. Applying DD to an object has the real potential to visualize DNA on a substrate from which a DNA profile can be generated, but it is important to determine whether this staining will adversely affect other forensic investigational techniques and vice versa. The aim of this study was to examine the interactions between staining a fingermark to detect DNA and then generate a DNA profile in combination with several standard latent fingermark enhancement methods. Six common fingerprint enhancements processes were chosen; (1)black powder, (2)black magnetic powder, (3)red magnetic powder, (4)white powder, (5)aluminum powder and (6)cyanoacrylate fuming. For all six methods, mark enhancement was carried out before DD staining and vice versa. DD is effective in detection of DNA in the presence of both aluminum and white finger mark powders and DD does not compromise the subsequent detection of ridge patterns if DD is applied first. Whilst magnetic powders could be used to successfully enhance latent fingermarks even after DD had been applied to them, latent DNA could not be observed in the marks irrespective of whether magnetic powder was applied before or after DD treatment. Magnetic powders did not adversely affect the profiling of DNA present in the marks. The application of DD to fingermarks did not adversely affect the enhancement of fingermarks using cyanoacrylate fuming. Whilst fluorescent particles resembling cells stained with DD were observed in marks either post-treated or pre-treated with cyanoacrylate vapor, DNA amplification and profiling was not successful. While it may be important in particular investigations to collect DNA profiles from latent fingermarks with continuous ridges and clear minutiae, the main utility of the technique described here would be in relation to investigations where enhancement has resulted in only partial or smudged marks. The results presented here indicate that if it is desirable to visualize latent DNA on an object but it is also planned to treat the object with cyanoacrylate vapor or magnetic powders then it is important to apply DD first, record the location of DNA and then apply the mark enhancement technique. For aluminum and white powder mark treatments such precautions are not important.

AB - A novel method for detection and visualization of latent DNA using Diamond™ Nucleic Acid Dye (DD)staining has been developed. Applying DD to an object has the real potential to visualize DNA on a substrate from which a DNA profile can be generated, but it is important to determine whether this staining will adversely affect other forensic investigational techniques and vice versa. The aim of this study was to examine the interactions between staining a fingermark to detect DNA and then generate a DNA profile in combination with several standard latent fingermark enhancement methods. Six common fingerprint enhancements processes were chosen; (1)black powder, (2)black magnetic powder, (3)red magnetic powder, (4)white powder, (5)aluminum powder and (6)cyanoacrylate fuming. For all six methods, mark enhancement was carried out before DD staining and vice versa. DD is effective in detection of DNA in the presence of both aluminum and white finger mark powders and DD does not compromise the subsequent detection of ridge patterns if DD is applied first. Whilst magnetic powders could be used to successfully enhance latent fingermarks even after DD had been applied to them, latent DNA could not be observed in the marks irrespective of whether magnetic powder was applied before or after DD treatment. Magnetic powders did not adversely affect the profiling of DNA present in the marks. The application of DD to fingermarks did not adversely affect the enhancement of fingermarks using cyanoacrylate fuming. Whilst fluorescent particles resembling cells stained with DD were observed in marks either post-treated or pre-treated with cyanoacrylate vapor, DNA amplification and profiling was not successful. While it may be important in particular investigations to collect DNA profiles from latent fingermarks with continuous ridges and clear minutiae, the main utility of the technique described here would be in relation to investigations where enhancement has resulted in only partial or smudged marks. The results presented here indicate that if it is desirable to visualize latent DNA on an object but it is also planned to treat the object with cyanoacrylate vapor or magnetic powders then it is important to apply DD first, record the location of DNA and then apply the mark enhancement technique. For aluminum and white powder mark treatments such precautions are not important.

KW - Diamond™ nucleic acid dye

KW - Fingermark detection

KW - Fingermark identification

KW - Fingerprint powder

KW - Stained cellular material

UR - http://www.scopus.com/inward/record.url?scp=85065305090&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/enhancement-fingermarks-visualizing-dna

U2 - 10.1016/j.forsciint.2019.04.035

DO - 10.1016/j.forsciint.2019.04.035

M3 - Article

VL - 300

SP - 99

EP - 105

JO - Forensic Science

JF - Forensic Science

SN - 0379-0738

ER -