Enhancing viral vaccine production using engineered knockout vero cell lines – A second look

F. Hoeksema, J. Karpilow, A. Luitjens, F. Lagerwerf, M. Havenga, M. Groothuizen, G. Gillissen, A. A.C. Lemckert, B. Jiang, R. A. Tripp, C. Yallop

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

The global adoption of vaccines to combat disease is hampered by the high cost of vaccine manufacturing. The work described herein follows two previous publications (van der Sanden et al., 2016; Wu et al., 2017) that report a strategy to enhance poliovirus and rotavirus vaccine production through genetic modification of the Vero cell lines used in large-scale vaccine manufacturing. CRISPR/Cas9 gene editing tools were used to knockout Vero target genes previously shown to play a role in polio- and rotavirus production. Subsequently, small-scale models of current industry manufacturing systems were developed and adopted to assess the increases in polio- and rotavirus output by multiple stable knockout cell lines. Unlike previous studies, the Vero knockout cell lines failed to achieve desired target yield increases. These findings suggest that additional research will be required before implementing the genetically engineered Vero cell lines in the manufacturing process for polio- and rotavirus vaccines to be able to supply vaccines at reduced prices.

Original languageEnglish
Pages (from-to)2093-2103
Number of pages11
JournalVaccine
Volume36
Issue number16
DOIs
Publication statusPublished - 12 Apr 2018
Externally publishedYes

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