Eosinophils contribute to innate antiviral immunity and promote clearance of respiratory syncytial virus

Simon Phipps, Chuan Lam, Suresh Mahalingam, Matthew Newhouse, Helene Rosenberg, Paul Foster, Klaus Matthaei

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Eosinophils are recruited to the lungs in response to respiratory syncytial virus (RSV) infection; however, their role in promoting antiviral host defense remains unclear. Here, we demonstrate that eosinophils express TLRs that recognize viral nucleic acids, are activated and degranulate after single-stranded RNA (ssRNA) stimulation of the TLR-7-MyD88 pathway, and provide host defense against RSV that is MyD88 dependent. In contrast to wild-type mice, virus clearance from lung tissue was more rapid in hypereosinophilic (interleukin-5 transgenic) mice. Transfer of wild-type but not MyD88-deficient eosinophils to the lungs of RSV-infected wild-type mice accelerated virus clearance and inhibited the development of airways hyperreactivity. Similar responses were observed when infected recipient mice were MyD88 deficient. Eosinophils isolated from infected hypereosinophilic MyD88-sufficient but not MyD88-deficient mice expressed greater amounts of IFN regulatory factor (IRF)-7 and eosinophil-associated ribonucleases EAR-1 and EAR-2. Hypereosinophilia in the airways of infected mice also correlated with increased expression of IRF-7, IFN-beta, and NOS-2, and inhibition of NO production with the NOS-2 inhibitor L-NMA partially reversed the accelerated virus clearance promoted by eosinophils. Collectively, our results demonstrate that eosinophils can protect against RSV in vivo, as they promote virus clearance and may thus limit virus-induced lung dysfunction
Original languageEnglish
Pages (from-to)1578-1586
Number of pages9
JournalBlood
Volume110
Issue number5
DOIs
Publication statusPublished - 2007

Fingerprint

Respiratory Syncytial Viruses
Viruses
Eosinophils
Innate Immunity
Antiviral Agents
Lung
Eosinophil Cationic Protein
Respiratory Syncytial Virus Infections
Interleukin-5
Bridge clearances
Nucleic Acids
Transgenic Mice
Interleukin-6
RNA
Tissue

Cite this

Phipps, S., Lam, C., Mahalingam, S., Newhouse, M., Rosenberg, H., Foster, P., & Matthaei, K. (2007). Eosinophils contribute to innate antiviral immunity and promote clearance of respiratory syncytial virus. Blood, 110(5), 1578-1586. https://doi.org/10.1182/blood-2007-01-071340
Phipps, Simon ; Lam, Chuan ; Mahalingam, Suresh ; Newhouse, Matthew ; Rosenberg, Helene ; Foster, Paul ; Matthaei, Klaus. / Eosinophils contribute to innate antiviral immunity and promote clearance of respiratory syncytial virus. In: Blood. 2007 ; Vol. 110, No. 5. pp. 1578-1586.
@article{c6934c059e7744c8a5f9993e1485dc8b,
title = "Eosinophils contribute to innate antiviral immunity and promote clearance of respiratory syncytial virus",
abstract = "Eosinophils are recruited to the lungs in response to respiratory syncytial virus (RSV) infection; however, their role in promoting antiviral host defense remains unclear. Here, we demonstrate that eosinophils express TLRs that recognize viral nucleic acids, are activated and degranulate after single-stranded RNA (ssRNA) stimulation of the TLR-7-MyD88 pathway, and provide host defense against RSV that is MyD88 dependent. In contrast to wild-type mice, virus clearance from lung tissue was more rapid in hypereosinophilic (interleukin-5 transgenic) mice. Transfer of wild-type but not MyD88-deficient eosinophils to the lungs of RSV-infected wild-type mice accelerated virus clearance and inhibited the development of airways hyperreactivity. Similar responses were observed when infected recipient mice were MyD88 deficient. Eosinophils isolated from infected hypereosinophilic MyD88-sufficient but not MyD88-deficient mice expressed greater amounts of IFN regulatory factor (IRF)-7 and eosinophil-associated ribonucleases EAR-1 and EAR-2. Hypereosinophilia in the airways of infected mice also correlated with increased expression of IRF-7, IFN-beta, and NOS-2, and inhibition of NO production with the NOS-2 inhibitor L-NMA partially reversed the accelerated virus clearance promoted by eosinophils. Collectively, our results demonstrate that eosinophils can protect against RSV in vivo, as they promote virus clearance and may thus limit virus-induced lung dysfunction",
author = "Simon Phipps and Chuan Lam and Suresh Mahalingam and Matthew Newhouse and Helene Rosenberg and Paul Foster and Klaus Matthaei",
year = "2007",
doi = "10.1182/blood-2007-01-071340",
language = "English",
volume = "110",
pages = "1578--1586",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "5",

}

Phipps, S, Lam, C, Mahalingam, S, Newhouse, M, Rosenberg, H, Foster, P & Matthaei, K 2007, 'Eosinophils contribute to innate antiviral immunity and promote clearance of respiratory syncytial virus', Blood, vol. 110, no. 5, pp. 1578-1586. https://doi.org/10.1182/blood-2007-01-071340

Eosinophils contribute to innate antiviral immunity and promote clearance of respiratory syncytial virus. / Phipps, Simon; Lam, Chuan; Mahalingam, Suresh; Newhouse, Matthew; Rosenberg, Helene; Foster, Paul; Matthaei, Klaus.

In: Blood, Vol. 110, No. 5, 2007, p. 1578-1586.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Eosinophils contribute to innate antiviral immunity and promote clearance of respiratory syncytial virus

AU - Phipps, Simon

AU - Lam, Chuan

AU - Mahalingam, Suresh

AU - Newhouse, Matthew

AU - Rosenberg, Helene

AU - Foster, Paul

AU - Matthaei, Klaus

PY - 2007

Y1 - 2007

N2 - Eosinophils are recruited to the lungs in response to respiratory syncytial virus (RSV) infection; however, their role in promoting antiviral host defense remains unclear. Here, we demonstrate that eosinophils express TLRs that recognize viral nucleic acids, are activated and degranulate after single-stranded RNA (ssRNA) stimulation of the TLR-7-MyD88 pathway, and provide host defense against RSV that is MyD88 dependent. In contrast to wild-type mice, virus clearance from lung tissue was more rapid in hypereosinophilic (interleukin-5 transgenic) mice. Transfer of wild-type but not MyD88-deficient eosinophils to the lungs of RSV-infected wild-type mice accelerated virus clearance and inhibited the development of airways hyperreactivity. Similar responses were observed when infected recipient mice were MyD88 deficient. Eosinophils isolated from infected hypereosinophilic MyD88-sufficient but not MyD88-deficient mice expressed greater amounts of IFN regulatory factor (IRF)-7 and eosinophil-associated ribonucleases EAR-1 and EAR-2. Hypereosinophilia in the airways of infected mice also correlated with increased expression of IRF-7, IFN-beta, and NOS-2, and inhibition of NO production with the NOS-2 inhibitor L-NMA partially reversed the accelerated virus clearance promoted by eosinophils. Collectively, our results demonstrate that eosinophils can protect against RSV in vivo, as they promote virus clearance and may thus limit virus-induced lung dysfunction

AB - Eosinophils are recruited to the lungs in response to respiratory syncytial virus (RSV) infection; however, their role in promoting antiviral host defense remains unclear. Here, we demonstrate that eosinophils express TLRs that recognize viral nucleic acids, are activated and degranulate after single-stranded RNA (ssRNA) stimulation of the TLR-7-MyD88 pathway, and provide host defense against RSV that is MyD88 dependent. In contrast to wild-type mice, virus clearance from lung tissue was more rapid in hypereosinophilic (interleukin-5 transgenic) mice. Transfer of wild-type but not MyD88-deficient eosinophils to the lungs of RSV-infected wild-type mice accelerated virus clearance and inhibited the development of airways hyperreactivity. Similar responses were observed when infected recipient mice were MyD88 deficient. Eosinophils isolated from infected hypereosinophilic MyD88-sufficient but not MyD88-deficient mice expressed greater amounts of IFN regulatory factor (IRF)-7 and eosinophil-associated ribonucleases EAR-1 and EAR-2. Hypereosinophilia in the airways of infected mice also correlated with increased expression of IRF-7, IFN-beta, and NOS-2, and inhibition of NO production with the NOS-2 inhibitor L-NMA partially reversed the accelerated virus clearance promoted by eosinophils. Collectively, our results demonstrate that eosinophils can protect against RSV in vivo, as they promote virus clearance and may thus limit virus-induced lung dysfunction

U2 - 10.1182/blood-2007-01-071340

DO - 10.1182/blood-2007-01-071340

M3 - Article

VL - 110

SP - 1578

EP - 1586

JO - Blood

JF - Blood

SN - 0006-4971

IS - 5

ER -