Establishing cause-effect relationship in hydrocarbon-contaminated sediments using a sublethal response of the benthic marine alga, Entomoneis CF Punctulata

Stuart Simpson, Tina Micevska, Bill Maher, Merrin Adams, Alexandria Stone

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    A sublethal whole-sediment toxicity test that uses flow cytometry to measure inhibition of esterase activity in the marine microalga Entomoneis cf punctulata was applied to the assessment of hydrocarbon-contaminated sediments and toxicity identification and evaluation (TIE). Concentration–response relationships were developed, and a 20% effect concentration for total polycyclic aromatic hydrocarbons (PAHs) of 60 mg/kg normalized to 1% total organic carbon was calculated. Relationships between toxic effects and sediment organic carbon concentrations, organic carbon forms (e.g., black carbon), and sediment particle size indicated that further normalization of hydrocarbon concentrations to sediment particle size may improve concentration–responserelationships. The algal toxicity test was applied as a rapid whole-sediment TIE procedure that involved the addition to sediment of powdered coconut charcoal (PCC), a hydrophobic, carbon-based material that strongly adsorbs PAHs and decreases the pore-water exposure pathway. Sediments with PCC concentrations of up to 15% (w/w) provided acceptable responses in control sediments. For six sediments with total PAH concentrations of 1,060, 4,060, 5,120, 9,150, 9,900, and 15,900 mg/kg, inhibition of E. cf punctulata esterase activity (% of control) was 75, 97, 94, 93, 100, and 97%, respectively. Following a 15% PCC amendment to these sediments, inhibition of esterase activity was 0, 1, 11, 69, 32, and 68%, respectively, indicating a decrease in toxicity in all sediments. Because the alga E. cf punctulata is exposed to toxicants via both pore water and overlying water, the reduction in toxicity achieved by 15% PCC additions can be related to the efficient removal of dissolved hydrocarbons released from sediment particles. The sediment–PCC manipulations coupled with algal whole-sediment toxicity tests provided an effective and rapid TIE method to determine whether hydrocarbon contaminants are responsible for toxicity in sediments
    Original languageEnglish
    Pages (from-to)163-170
    Number of pages8
    JournalEnvironmental Toxicology and Chemistry
    Volume26
    Issue number1
    DOIs
    Publication statusPublished - 2007

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    Cocos
    Charcoal
    Algae
    Hydrocarbons
    Toxicity Tests
    Polycyclic Aromatic Hydrocarbons
    Sediments
    Esterases
    Carbon
    alga
    hydrocarbon
    Toxicity
    Particle Size
    sediment
    Water
    Soot
    Poisons
    toxicity
    charcoal
    Flow Cytometry

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    title = "Establishing cause-effect relationship in hydrocarbon-contaminated sediments using a sublethal response of the benthic marine alga, Entomoneis CF Punctulata",
    abstract = "A sublethal whole-sediment toxicity test that uses flow cytometry to measure inhibition of esterase activity in the marine microalga Entomoneis cf punctulata was applied to the assessment of hydrocarbon-contaminated sediments and toxicity identification and evaluation (TIE). Concentration–response relationships were developed, and a 20{\%} effect concentration for total polycyclic aromatic hydrocarbons (PAHs) of 60 mg/kg normalized to 1{\%} total organic carbon was calculated. Relationships between toxic effects and sediment organic carbon concentrations, organic carbon forms (e.g., black carbon), and sediment particle size indicated that further normalization of hydrocarbon concentrations to sediment particle size may improve concentration–responserelationships. The algal toxicity test was applied as a rapid whole-sediment TIE procedure that involved the addition to sediment of powdered coconut charcoal (PCC), a hydrophobic, carbon-based material that strongly adsorbs PAHs and decreases the pore-water exposure pathway. Sediments with PCC concentrations of up to 15{\%} (w/w) provided acceptable responses in control sediments. For six sediments with total PAH concentrations of 1,060, 4,060, 5,120, 9,150, 9,900, and 15,900 mg/kg, inhibition of E. cf punctulata esterase activity ({\%} of control) was 75, 97, 94, 93, 100, and 97{\%}, respectively. Following a 15{\%} PCC amendment to these sediments, inhibition of esterase activity was 0, 1, 11, 69, 32, and 68{\%}, respectively, indicating a decrease in toxicity in all sediments. Because the alga E. cf punctulata is exposed to toxicants via both pore water and overlying water, the reduction in toxicity achieved by 15{\%} PCC additions can be related to the efficient removal of dissolved hydrocarbons released from sediment particles. The sediment–PCC manipulations coupled with algal whole-sediment toxicity tests provided an effective and rapid TIE method to determine whether hydrocarbon contaminants are responsible for toxicity in sediments",
    author = "Stuart Simpson and Tina Micevska and Bill Maher and Merrin Adams and Alexandria Stone",
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    language = "English",
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    pages = "163--170",
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    Establishing cause-effect relationship in hydrocarbon-contaminated sediments using a sublethal response of the benthic marine alga, Entomoneis CF Punctulata. / Simpson, Stuart; Micevska, Tina; Maher, Bill; Adams, Merrin; Stone, Alexandria.

    In: Environmental Toxicology and Chemistry, Vol. 26, No. 1, 2007, p. 163-170.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - Stone, Alexandria

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    AB - A sublethal whole-sediment toxicity test that uses flow cytometry to measure inhibition of esterase activity in the marine microalga Entomoneis cf punctulata was applied to the assessment of hydrocarbon-contaminated sediments and toxicity identification and evaluation (TIE). Concentration–response relationships were developed, and a 20% effect concentration for total polycyclic aromatic hydrocarbons (PAHs) of 60 mg/kg normalized to 1% total organic carbon was calculated. Relationships between toxic effects and sediment organic carbon concentrations, organic carbon forms (e.g., black carbon), and sediment particle size indicated that further normalization of hydrocarbon concentrations to sediment particle size may improve concentration–responserelationships. The algal toxicity test was applied as a rapid whole-sediment TIE procedure that involved the addition to sediment of powdered coconut charcoal (PCC), a hydrophobic, carbon-based material that strongly adsorbs PAHs and decreases the pore-water exposure pathway. Sediments with PCC concentrations of up to 15% (w/w) provided acceptable responses in control sediments. For six sediments with total PAH concentrations of 1,060, 4,060, 5,120, 9,150, 9,900, and 15,900 mg/kg, inhibition of E. cf punctulata esterase activity (% of control) was 75, 97, 94, 93, 100, and 97%, respectively. Following a 15% PCC amendment to these sediments, inhibition of esterase activity was 0, 1, 11, 69, 32, and 68%, respectively, indicating a decrease in toxicity in all sediments. Because the alga E. cf punctulata is exposed to toxicants via both pore water and overlying water, the reduction in toxicity achieved by 15% PCC additions can be related to the efficient removal of dissolved hydrocarbons released from sediment particles. The sediment–PCC manipulations coupled with algal whole-sediment toxicity tests provided an effective and rapid TIE method to determine whether hydrocarbon contaminants are responsible for toxicity in sediments

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    SN - 0730-7268

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