Exercise-recruited NK cells display exercise-associated eHSP-70

Peggy Horn, Amelia Kalz, Chin Leong Lim, David Pyne, Philo Saunders, Laurel Mackinnon, Jonathon Peake, Katsushiko Suzuki

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    It is hypothesized that increased plasma or serum concentrations of extracellular heat shock proteins (eHSP) serve as a danger signal to the innate immune system. Cellular binding of eHSP leads to activation of NK cells and monocytes, as measured by their increased cytokine production, mitotic division and killing capacity. We examined whether eHSP binds to NK lymphocytes in vivo in athletes performing endurance exercise in the heat. Eighteen trained male runners ran at 70% VO2max at 35 degrees C and 40% relative humidity. Venous blood collected before, after and 1.5 h after exercise was analysed for leukocyte distribution, phenotype and eHSP70. NK cell-enriched samples were examined for co-localization of CD94 and eHSP70 expression. Plasma eHSP-70 concentration was measured by ELISA. Subjects ran for approximately 50 min, which elicited a reversible leukocytosis. NK cell count increased 83% (p < 0.01) immediately after exercise, then decreased to 66% of the resting level 1.5 h after exercise (p < 0.05). Plasma eHSP concentration increased 167% after exercise and remained elevated (by up to 71%) 1.5 h after exercise (p < 0.01). eHSP was expressed on both NK cells and monocytes at all times; the count of NK cells positive for eHSP doubled from 0.04 +/- 0.02 10(9)/L (mean +/- SD) to 0.08 +/- 0.06 10(9)/L after exercise. In summary, exercise in the heat increased free plasma eHSP concentration, and the eHSP co-localized with CD94 on NK cells. These data confirm the link between exercise and activation of the innate immune system.

    Original languageEnglish
    Pages (from-to)100-11
    Number of pages12
    JournalExercise Immunology Review
    Volume13
    Publication statusPublished - 2007

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    HSP70 Heat-Shock Proteins
    Natural Killer Cells
    Heat-Shock Proteins
    Exercise
    Monocytes
    Immune System
    Hot Temperature
    Leukocytosis
    Humidity
    Athletes
    Leukocytes
    Cell Count
    Enzyme-Linked Immunosorbent Assay
    Lymphocytes
    Cytokines
    Phenotype

    Cite this

    Horn, P., Kalz, A., Lim, C. L., Pyne, D., Saunders, P., Mackinnon, L., ... Suzuki, K. (2007). Exercise-recruited NK cells display exercise-associated eHSP-70. Exercise Immunology Review, 13, 100-11.
    Horn, Peggy ; Kalz, Amelia ; Lim, Chin Leong ; Pyne, David ; Saunders, Philo ; Mackinnon, Laurel ; Peake, Jonathon ; Suzuki, Katsushiko. / Exercise-recruited NK cells display exercise-associated eHSP-70. In: Exercise Immunology Review. 2007 ; Vol. 13. pp. 100-11.
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    abstract = "It is hypothesized that increased plasma or serum concentrations of extracellular heat shock proteins (eHSP) serve as a danger signal to the innate immune system. Cellular binding of eHSP leads to activation of NK cells and monocytes, as measured by their increased cytokine production, mitotic division and killing capacity. We examined whether eHSP binds to NK lymphocytes in vivo in athletes performing endurance exercise in the heat. Eighteen trained male runners ran at 70{\%} VO2max at 35 degrees C and 40{\%} relative humidity. Venous blood collected before, after and 1.5 h after exercise was analysed for leukocyte distribution, phenotype and eHSP70. NK cell-enriched samples were examined for co-localization of CD94 and eHSP70 expression. Plasma eHSP-70 concentration was measured by ELISA. Subjects ran for approximately 50 min, which elicited a reversible leukocytosis. NK cell count increased 83{\%} (p < 0.01) immediately after exercise, then decreased to 66{\%} of the resting level 1.5 h after exercise (p < 0.05). Plasma eHSP concentration increased 167{\%} after exercise and remained elevated (by up to 71{\%}) 1.5 h after exercise (p < 0.01). eHSP was expressed on both NK cells and monocytes at all times; the count of NK cells positive for eHSP doubled from 0.04 +/- 0.02 10(9)/L (mean +/- SD) to 0.08 +/- 0.06 10(9)/L after exercise. In summary, exercise in the heat increased free plasma eHSP concentration, and the eHSP co-localized with CD94 on NK cells. These data confirm the link between exercise and activation of the innate immune system.",
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    author = "Peggy Horn and Amelia Kalz and Lim, {Chin Leong} and David Pyne and Philo Saunders and Laurel Mackinnon and Jonathon Peake and Katsushiko Suzuki",
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    Horn, P, Kalz, A, Lim, CL, Pyne, D, Saunders, P, Mackinnon, L, Peake, J & Suzuki, K 2007, 'Exercise-recruited NK cells display exercise-associated eHSP-70', Exercise Immunology Review, vol. 13, pp. 100-11.

    Exercise-recruited NK cells display exercise-associated eHSP-70. / Horn, Peggy; Kalz, Amelia; Lim, Chin Leong; Pyne, David; Saunders, Philo; Mackinnon, Laurel; Peake, Jonathon; Suzuki, Katsushiko.

    In: Exercise Immunology Review, Vol. 13, 2007, p. 100-11.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Exercise-recruited NK cells display exercise-associated eHSP-70

    AU - Horn, Peggy

    AU - Kalz, Amelia

    AU - Lim, Chin Leong

    AU - Pyne, David

    AU - Saunders, Philo

    AU - Mackinnon, Laurel

    AU - Peake, Jonathon

    AU - Suzuki, Katsushiko

    PY - 2007

    Y1 - 2007

    N2 - It is hypothesized that increased plasma or serum concentrations of extracellular heat shock proteins (eHSP) serve as a danger signal to the innate immune system. Cellular binding of eHSP leads to activation of NK cells and monocytes, as measured by their increased cytokine production, mitotic division and killing capacity. We examined whether eHSP binds to NK lymphocytes in vivo in athletes performing endurance exercise in the heat. Eighteen trained male runners ran at 70% VO2max at 35 degrees C and 40% relative humidity. Venous blood collected before, after and 1.5 h after exercise was analysed for leukocyte distribution, phenotype and eHSP70. NK cell-enriched samples were examined for co-localization of CD94 and eHSP70 expression. Plasma eHSP-70 concentration was measured by ELISA. Subjects ran for approximately 50 min, which elicited a reversible leukocytosis. NK cell count increased 83% (p < 0.01) immediately after exercise, then decreased to 66% of the resting level 1.5 h after exercise (p < 0.05). Plasma eHSP concentration increased 167% after exercise and remained elevated (by up to 71%) 1.5 h after exercise (p < 0.01). eHSP was expressed on both NK cells and monocytes at all times; the count of NK cells positive for eHSP doubled from 0.04 +/- 0.02 10(9)/L (mean +/- SD) to 0.08 +/- 0.06 10(9)/L after exercise. In summary, exercise in the heat increased free plasma eHSP concentration, and the eHSP co-localized with CD94 on NK cells. These data confirm the link between exercise and activation of the innate immune system.

    AB - It is hypothesized that increased plasma or serum concentrations of extracellular heat shock proteins (eHSP) serve as a danger signal to the innate immune system. Cellular binding of eHSP leads to activation of NK cells and monocytes, as measured by their increased cytokine production, mitotic division and killing capacity. We examined whether eHSP binds to NK lymphocytes in vivo in athletes performing endurance exercise in the heat. Eighteen trained male runners ran at 70% VO2max at 35 degrees C and 40% relative humidity. Venous blood collected before, after and 1.5 h after exercise was analysed for leukocyte distribution, phenotype and eHSP70. NK cell-enriched samples were examined for co-localization of CD94 and eHSP70 expression. Plasma eHSP-70 concentration was measured by ELISA. Subjects ran for approximately 50 min, which elicited a reversible leukocytosis. NK cell count increased 83% (p < 0.01) immediately after exercise, then decreased to 66% of the resting level 1.5 h after exercise (p < 0.05). Plasma eHSP concentration increased 167% after exercise and remained elevated (by up to 71%) 1.5 h after exercise (p < 0.01). eHSP was expressed on both NK cells and monocytes at all times; the count of NK cells positive for eHSP doubled from 0.04 +/- 0.02 10(9)/L (mean +/- SD) to 0.08 +/- 0.06 10(9)/L after exercise. In summary, exercise in the heat increased free plasma eHSP concentration, and the eHSP co-localized with CD94 on NK cells. These data confirm the link between exercise and activation of the innate immune system.

    KW - Adult

    KW - Exercise

    KW - Exercise Test

    KW - HSP70 Heat-Shock Proteins

    KW - Humans

    KW - Immunity, Cellular

    KW - Killer Cells, Natural

    KW - Leukocyte Count

    KW - Male

    KW - Monocytes

    KW - Reference Values

    KW - Journal Article

    KW - Research Support, Non-U.S. Gov't

    M3 - Article

    VL - 13

    SP - 100

    EP - 111

    JO - Exercise Immunology Review

    JF - Exercise Immunology Review

    SN - 1077-5552

    ER -

    Horn P, Kalz A, Lim CL, Pyne D, Saunders P, Mackinnon L et al. Exercise-recruited NK cells display exercise-associated eHSP-70. Exercise Immunology Review. 2007;13:100-11.