Experimental infection of self-cured Leiopelma archeyi with the amphibian chytrid Batrachochytrium dendrobatidis

Stephanie Shaw, Phillip Bishop, Lee Berger, Lee Skerratt, Stephen Fritzdorf, Dianne Gleeson, Amanda Haigh, Sarah Herbert, R Speare

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    The susceptibility of Archey’s frog Leiopelma archeyi to Batrachochytrium dendrobatidis (Bd) is unknown, although one large population is thought to have declined sharply due to chytridiomycosis. As primary infection experiments were not permitted in this endangered New Zealand species, 6 wild-caught L. archeyi that naturally cleared infections with Bd while in captivity were exposed again to Bd to assess their immunity. These frogs were from an infected population at Whareorino, which has no known declines. All 6 L. archeyi became reinfected at low intensities, but rapidly self cured, most by 2 wk. Six Litoria ewingii were used as positive controls and developed heavier infections and clinical signs by 3 wk, demonstrating that the zoospore inoculum was virulent. Six negative controls of each species remained uninfected and healthy. Our results show that L. archeyi that have self cured have resistance to chytridiomycosis when exposed. The pattern is consistent with innate or acquired immunity to Bd, and immunological studies are needed to confirm this.
    Original languageEnglish
    Pages (from-to)159-163
    Number of pages5
    JournalDiseases of Aquatic Organisms
    Volume92
    DOIs
    Publication statusPublished - 2010

    Fingerprint

    amphibian
    amphibians
    immunity
    frog
    frogs
    infection
    zoospores
    captivity
    inoculum
    new species
    experiment
    Litoria
    adaptive immunity
    innate immunity

    Cite this

    Shaw, Stephanie ; Bishop, Phillip ; Berger, Lee ; Skerratt, Lee ; Fritzdorf, Stephen ; Gleeson, Dianne ; Haigh, Amanda ; Herbert, Sarah ; Speare, R. / Experimental infection of self-cured Leiopelma archeyi with the amphibian chytrid Batrachochytrium dendrobatidis. In: Diseases of Aquatic Organisms. 2010 ; Vol. 92. pp. 159-163.
    @article{b06bd21d4dd14e5f9d881a6005a05a14,
    title = "Experimental infection of self-cured Leiopelma archeyi with the amphibian chytrid Batrachochytrium dendrobatidis",
    abstract = "The susceptibility of Archey’s frog Leiopelma archeyi to Batrachochytrium dendrobatidis (Bd) is unknown, although one large population is thought to have declined sharply due to chytridiomycosis. As primary infection experiments were not permitted in this endangered New Zealand species, 6 wild-caught L. archeyi that naturally cleared infections with Bd while in captivity were exposed again to Bd to assess their immunity. These frogs were from an infected population at Whareorino, which has no known declines. All 6 L. archeyi became reinfected at low intensities, but rapidly self cured, most by 2 wk. Six Litoria ewingii were used as positive controls and developed heavier infections and clinical signs by 3 wk, demonstrating that the zoospore inoculum was virulent. Six negative controls of each species remained uninfected and healthy. Our results show that L. archeyi that have self cured have resistance to chytridiomycosis when exposed. The pattern is consistent with innate or acquired immunity to Bd, and immunological studies are needed to confirm this.",
    keywords = "Leiopelma archeyi, Litoria ewingii, Batrachochytrium dendrobatidis, Chytridiomycosis.",
    author = "Stephanie Shaw and Phillip Bishop and Lee Berger and Lee Skerratt and Stephen Fritzdorf and Dianne Gleeson and Amanda Haigh and Sarah Herbert and R Speare",
    year = "2010",
    doi = "10.3354/dao02227",
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    Shaw, S, Bishop, P, Berger, L, Skerratt, L, Fritzdorf, S, Gleeson, D, Haigh, A, Herbert, S & Speare, R 2010, 'Experimental infection of self-cured Leiopelma archeyi with the amphibian chytrid Batrachochytrium dendrobatidis', Diseases of Aquatic Organisms, vol. 92, pp. 159-163. https://doi.org/10.3354/dao02227

    Experimental infection of self-cured Leiopelma archeyi with the amphibian chytrid Batrachochytrium dendrobatidis. / Shaw, Stephanie; Bishop, Phillip; Berger, Lee; Skerratt, Lee; Fritzdorf, Stephen; Gleeson, Dianne; Haigh, Amanda; Herbert, Sarah; Speare, R.

    In: Diseases of Aquatic Organisms, Vol. 92, 2010, p. 159-163.

    Research output: Contribution to journalArticle

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    T1 - Experimental infection of self-cured Leiopelma archeyi with the amphibian chytrid Batrachochytrium dendrobatidis

    AU - Shaw, Stephanie

    AU - Bishop, Phillip

    AU - Berger, Lee

    AU - Skerratt, Lee

    AU - Fritzdorf, Stephen

    AU - Gleeson, Dianne

    AU - Haigh, Amanda

    AU - Herbert, Sarah

    AU - Speare, R

    PY - 2010

    Y1 - 2010

    N2 - The susceptibility of Archey’s frog Leiopelma archeyi to Batrachochytrium dendrobatidis (Bd) is unknown, although one large population is thought to have declined sharply due to chytridiomycosis. As primary infection experiments were not permitted in this endangered New Zealand species, 6 wild-caught L. archeyi that naturally cleared infections with Bd while in captivity were exposed again to Bd to assess their immunity. These frogs were from an infected population at Whareorino, which has no known declines. All 6 L. archeyi became reinfected at low intensities, but rapidly self cured, most by 2 wk. Six Litoria ewingii were used as positive controls and developed heavier infections and clinical signs by 3 wk, demonstrating that the zoospore inoculum was virulent. Six negative controls of each species remained uninfected and healthy. Our results show that L. archeyi that have self cured have resistance to chytridiomycosis when exposed. The pattern is consistent with innate or acquired immunity to Bd, and immunological studies are needed to confirm this.

    AB - The susceptibility of Archey’s frog Leiopelma archeyi to Batrachochytrium dendrobatidis (Bd) is unknown, although one large population is thought to have declined sharply due to chytridiomycosis. As primary infection experiments were not permitted in this endangered New Zealand species, 6 wild-caught L. archeyi that naturally cleared infections with Bd while in captivity were exposed again to Bd to assess their immunity. These frogs were from an infected population at Whareorino, which has no known declines. All 6 L. archeyi became reinfected at low intensities, but rapidly self cured, most by 2 wk. Six Litoria ewingii were used as positive controls and developed heavier infections and clinical signs by 3 wk, demonstrating that the zoospore inoculum was virulent. Six negative controls of each species remained uninfected and healthy. Our results show that L. archeyi that have self cured have resistance to chytridiomycosis when exposed. The pattern is consistent with innate or acquired immunity to Bd, and immunological studies are needed to confirm this.

    KW - Leiopelma archeyi

    KW - Litoria ewingii

    KW - Batrachochytrium dendrobatidis

    KW - Chytridiomycosis.

    U2 - 10.3354/dao02227

    DO - 10.3354/dao02227

    M3 - Article

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    EP - 163

    JO - Diseases of Aquatic Organisms

    JF - Diseases of Aquatic Organisms

    SN - 0177-5103

    ER -