Exposure-dose-response of Anadara trapezia to metal contaminated estuarine sediments. 2. Lead spiked sediments

Anne Taylor, William Maher

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    Abstract

    The composition of near shore marine environments is increasingly being altered by contaminants from human activities. The ability of lead, which has no known biological function, to mimic biologically essential metals makes it one of the most toxic to marine biota. The relationship between lead exposure, dose and response was investigated in Anadara trapezia exposed for 56 days to lead spiked sediment (100 and 300 g/g dry mass). Lead tissue concentrations of the 300 g/g exposed A. trapezia doubled in the last 2 weeks of exposure with final lead tissue concentrations of exposed organisms of 1 and 12 g/g, respectively. Tissue lead accumulation of exposed organisms followed the pattern haemolymph > gill > hepatopancreas during much of the 56 day exposure. Between 30 and 69% of accumulated lead in the gill and hepatopancreas was detoxified and fairly evenly distributed between the metal rich granule and the metallothionein like protein fractions. Approximately half of the biologically active lead in both tissues was in the mitochondrial fraction which showed increased cytochrome c oxidase activity in lead exposed organisms. There was a reduction in GPx activity, an associated increase in total glutathione concentrations and reduced GSH:GSSG ratios due to a build up of oxidised glutathione. These changes in the glutathione pathway were reflected in the total antioxidant capacity of lead exposed A. trapezia which were significantly reduced compared to control organisms. Increased lead exposure significantly increased lipid peroxidation, lysosomal destabilisation and frequency of micronuclei. A significant exposureâ¿¿doseâ¿¿response relationship for A. trapezia exposed to lead enriched sediments indicates that elevated sediment lead concentrations have the potential to increase biologically active lead burdens and impair the antioxidant reduction capacity leading to a series of associated effects from lipid peroxidation to cellular perturbation and genotoxic damage.
    Original languageEnglish
    Pages (from-to)79-89
    Number of pages11
    JournalAquatic Toxicology
    Volume116-117
    DOIs
    Publication statusPublished - 2012

    Fingerprint

    Arcidae
    Anadara
    estuarine sediments
    estuarine sediment
    dose response
    Metals
    metals
    sediments
    metal
    sediment
    organisms
    Hepatopancreas
    glutathione
    Glutathione Disulfide
    hepatopancreas
    antioxidant
    Lipid Peroxidation
    Glutathione
    gills
    lipid peroxidation

    Cite this

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    abstract = "The composition of near shore marine environments is increasingly being altered by contaminants from human activities. The ability of lead, which has no known biological function, to mimic biologically essential metals makes it one of the most toxic to marine biota. The relationship between lead exposure, dose and response was investigated in Anadara trapezia exposed for 56 days to lead spiked sediment (100 and 300 g/g dry mass). Lead tissue concentrations of the 300 g/g exposed A. trapezia doubled in the last 2 weeks of exposure with final lead tissue concentrations of exposed organisms of 1 and 12 g/g, respectively. Tissue lead accumulation of exposed organisms followed the pattern haemolymph > gill > hepatopancreas during much of the 56 day exposure. Between 30 and 69{\%} of accumulated lead in the gill and hepatopancreas was detoxified and fairly evenly distributed between the metal rich granule and the metallothionein like protein fractions. Approximately half of the biologically active lead in both tissues was in the mitochondrial fraction which showed increased cytochrome c oxidase activity in lead exposed organisms. There was a reduction in GPx activity, an associated increase in total glutathione concentrations and reduced GSH:GSSG ratios due to a build up of oxidised glutathione. These changes in the glutathione pathway were reflected in the total antioxidant capacity of lead exposed A. trapezia which were significantly reduced compared to control organisms. Increased lead exposure significantly increased lipid peroxidation, lysosomal destabilisation and frequency of micronuclei. A significant exposure{\^a}¿¿dose{\^a}¿¿response relationship for A. trapezia exposed to lead enriched sediments indicates that elevated sediment lead concentrations have the potential to increase biologically active lead burdens and impair the antioxidant reduction capacity leading to a series of associated effects from lipid peroxidation to cellular perturbation and genotoxic damage.",
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    Exposure-dose-response of Anadara trapezia to metal contaminated estuarine sediments. 2. Lead spiked sediments. / Taylor, Anne; Maher, William.

    In: Aquatic Toxicology, Vol. 116-117, 2012, p. 79-89.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Exposure-dose-response of Anadara trapezia to metal contaminated estuarine sediments. 2. Lead spiked sediments

    AU - Taylor, Anne

    AU - Maher, William

    PY - 2012

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    N2 - The composition of near shore marine environments is increasingly being altered by contaminants from human activities. The ability of lead, which has no known biological function, to mimic biologically essential metals makes it one of the most toxic to marine biota. The relationship between lead exposure, dose and response was investigated in Anadara trapezia exposed for 56 days to lead spiked sediment (100 and 300 g/g dry mass). Lead tissue concentrations of the 300 g/g exposed A. trapezia doubled in the last 2 weeks of exposure with final lead tissue concentrations of exposed organisms of 1 and 12 g/g, respectively. Tissue lead accumulation of exposed organisms followed the pattern haemolymph > gill > hepatopancreas during much of the 56 day exposure. Between 30 and 69% of accumulated lead in the gill and hepatopancreas was detoxified and fairly evenly distributed between the metal rich granule and the metallothionein like protein fractions. Approximately half of the biologically active lead in both tissues was in the mitochondrial fraction which showed increased cytochrome c oxidase activity in lead exposed organisms. There was a reduction in GPx activity, an associated increase in total glutathione concentrations and reduced GSH:GSSG ratios due to a build up of oxidised glutathione. These changes in the glutathione pathway were reflected in the total antioxidant capacity of lead exposed A. trapezia which were significantly reduced compared to control organisms. Increased lead exposure significantly increased lipid peroxidation, lysosomal destabilisation and frequency of micronuclei. A significant exposureâ¿¿doseâ¿¿response relationship for A. trapezia exposed to lead enriched sediments indicates that elevated sediment lead concentrations have the potential to increase biologically active lead burdens and impair the antioxidant reduction capacity leading to a series of associated effects from lipid peroxidation to cellular perturbation and genotoxic damage.

    AB - The composition of near shore marine environments is increasingly being altered by contaminants from human activities. The ability of lead, which has no known biological function, to mimic biologically essential metals makes it one of the most toxic to marine biota. The relationship between lead exposure, dose and response was investigated in Anadara trapezia exposed for 56 days to lead spiked sediment (100 and 300 g/g dry mass). Lead tissue concentrations of the 300 g/g exposed A. trapezia doubled in the last 2 weeks of exposure with final lead tissue concentrations of exposed organisms of 1 and 12 g/g, respectively. Tissue lead accumulation of exposed organisms followed the pattern haemolymph > gill > hepatopancreas during much of the 56 day exposure. Between 30 and 69% of accumulated lead in the gill and hepatopancreas was detoxified and fairly evenly distributed between the metal rich granule and the metallothionein like protein fractions. Approximately half of the biologically active lead in both tissues was in the mitochondrial fraction which showed increased cytochrome c oxidase activity in lead exposed organisms. There was a reduction in GPx activity, an associated increase in total glutathione concentrations and reduced GSH:GSSG ratios due to a build up of oxidised glutathione. These changes in the glutathione pathway were reflected in the total antioxidant capacity of lead exposed A. trapezia which were significantly reduced compared to control organisms. Increased lead exposure significantly increased lipid peroxidation, lysosomal destabilisation and frequency of micronuclei. A significant exposureâ¿¿doseâ¿¿response relationship for A. trapezia exposed to lead enriched sediments indicates that elevated sediment lead concentrations have the potential to increase biologically active lead burdens and impair the antioxidant reduction capacity leading to a series of associated effects from lipid peroxidation to cellular perturbation and genotoxic damage.

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    KW - Biomarkers

    KW - Biologically active metal

    KW - Biologically detoxified metal

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    KW - Lysosomal stability

    KW - Lipid peroxidation

    KW - Micronuclei

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    DO - 10.1016/J.AQUATOX.2012.02.017

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    SP - 79

    EP - 89

    JO - Acquayic Toxicology

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    SN - 0166-445X

    ER -