Exposure-dose-response of Tellina deltoidalis to metal contaminated estuarine sediments 2. Lead spiked sediments

Anne TAYLOR, Bill MAHER

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    Abstract

    Lead accumulation in estuarine sediments, as a result of activities such as mining and ore smelting, and through urban runoff is a continuing problem in the increasingly developed world. Marine organisms accumulate lead, which is known to be highly toxic to biological processes and to degrade organism and ecosystem health. Here the relationship between lead exposure, dose and response was investigated in the sediment dwelling, deposit feeding, marine bivalve Tellina deltoidalis. Bivalves were exposed in the laboratory to individual lead spiked sediments at b 0.01, 100 and 300 µg/g dry mass, for 28 days and accumulated total tissue lead concentrations of 4, 96 and 430 µg/g, respectively. Subcellular fractionation indicated that around 70% of the total accumulated tissue lead was detoxified, three quarters of the detoxified lead fraction was converted into metal rich granules, with the remainder in the metallothione in like protein fraction. The majority of biologically active lead was associated with the mitochondrial fraction with up to a 128 fold increase in lead burden in exposed organisms compared to controls. This indicates lead detoxification was occurring but the organism was unable to prevent lead interacting with sensitive organelles. With increased lead exposure T. deltoidalis showed a suppression in glutathione peroxidase activity, total glutathione concentration and reduced GSH:GSSG ratios, however, these differences were not significant. Lead exposed T. deltoidalis had a significantly reduced total antioxidant capacity which corresponded with increased lipid peroxidation, lysosomal destabilisation and micronuclei frequency. The exposure–dose–response relationships demonstrated for lead exposed T. deltoidalis supports its potential for the development of sublethal endpoints in lead toxicity assessment.
    Original languageEnglish
    Pages (from-to)52-61
    Number of pages10
    JournalComparative Biochemistry and Physiology. Part C: Toxicology Pharmacology
    Volume159
    DOIs
    Publication statusPublished - 2014

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    Sediments
    Metals
    Bivalvia
    Lead
    Tissue
    Biological Phenomena
    Aquatic Organisms
    Detoxification
    Glutathione Disulfide
    Poisons
    Smelting
    Fractionation
    Glutathione Peroxidase
    Runoff
    Organelles
    Ecosystems
    Lipid Peroxidation
    Ores
    Glutathione
    Ecosystem

    Cite this

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    title = "Exposure-dose-response of Tellina deltoidalis to metal contaminated estuarine sediments 2. Lead spiked sediments",
    abstract = "Lead accumulation in estuarine sediments, as a result of activities such as mining and ore smelting, and through urban runoff is a continuing problem in the increasingly developed world. Marine organisms accumulate lead, which is known to be highly toxic to biological processes and to degrade organism and ecosystem health. Here the relationship between lead exposure, dose and response was investigated in the sediment dwelling, deposit feeding, marine bivalve Tellina deltoidalis. Bivalves were exposed in the laboratory to individual lead spiked sediments at b 0.01, 100 and 300 µg/g dry mass, for 28 days and accumulated total tissue lead concentrations of 4, 96 and 430 µg/g, respectively. Subcellular fractionation indicated that around 70{\%} of the total accumulated tissue lead was detoxified, three quarters of the detoxified lead fraction was converted into metal rich granules, with the remainder in the metallothione in like protein fraction. The majority of biologically active lead was associated with the mitochondrial fraction with up to a 128 fold increase in lead burden in exposed organisms compared to controls. This indicates lead detoxification was occurring but the organism was unable to prevent lead interacting with sensitive organelles. With increased lead exposure T. deltoidalis showed a suppression in glutathione peroxidase activity, total glutathione concentration and reduced GSH:GSSG ratios, however, these differences were not significant. Lead exposed T. deltoidalis had a significantly reduced total antioxidant capacity which corresponded with increased lipid peroxidation, lysosomal destabilisation and micronuclei frequency. The exposure–dose–response relationships demonstrated for lead exposed T. deltoidalis supports its potential for the development of sublethal endpoints in lead toxicity assessment.",
    keywords = "Biomarkers, Subcellular lead, Oxidative stress, Lysosomes, Lipid peroxidation, Micronuclei, Bivalve.",
    author = "Anne TAYLOR and Bill MAHER",
    year = "2014",
    doi = "10.1016/j.cbpc.2013.09.006",
    language = "English",
    volume = "159",
    pages = "52--61",
    journal = "Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology",
    issn = "1532-0456",
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    }

    TY - JOUR

    T1 - Exposure-dose-response of Tellina deltoidalis to metal contaminated estuarine sediments 2. Lead spiked sediments

    AU - TAYLOR, Anne

    AU - MAHER, Bill

    PY - 2014

    Y1 - 2014

    N2 - Lead accumulation in estuarine sediments, as a result of activities such as mining and ore smelting, and through urban runoff is a continuing problem in the increasingly developed world. Marine organisms accumulate lead, which is known to be highly toxic to biological processes and to degrade organism and ecosystem health. Here the relationship between lead exposure, dose and response was investigated in the sediment dwelling, deposit feeding, marine bivalve Tellina deltoidalis. Bivalves were exposed in the laboratory to individual lead spiked sediments at b 0.01, 100 and 300 µg/g dry mass, for 28 days and accumulated total tissue lead concentrations of 4, 96 and 430 µg/g, respectively. Subcellular fractionation indicated that around 70% of the total accumulated tissue lead was detoxified, three quarters of the detoxified lead fraction was converted into metal rich granules, with the remainder in the metallothione in like protein fraction. The majority of biologically active lead was associated with the mitochondrial fraction with up to a 128 fold increase in lead burden in exposed organisms compared to controls. This indicates lead detoxification was occurring but the organism was unable to prevent lead interacting with sensitive organelles. With increased lead exposure T. deltoidalis showed a suppression in glutathione peroxidase activity, total glutathione concentration and reduced GSH:GSSG ratios, however, these differences were not significant. Lead exposed T. deltoidalis had a significantly reduced total antioxidant capacity which corresponded with increased lipid peroxidation, lysosomal destabilisation and micronuclei frequency. The exposure–dose–response relationships demonstrated for lead exposed T. deltoidalis supports its potential for the development of sublethal endpoints in lead toxicity assessment.

    AB - Lead accumulation in estuarine sediments, as a result of activities such as mining and ore smelting, and through urban runoff is a continuing problem in the increasingly developed world. Marine organisms accumulate lead, which is known to be highly toxic to biological processes and to degrade organism and ecosystem health. Here the relationship between lead exposure, dose and response was investigated in the sediment dwelling, deposit feeding, marine bivalve Tellina deltoidalis. Bivalves were exposed in the laboratory to individual lead spiked sediments at b 0.01, 100 and 300 µg/g dry mass, for 28 days and accumulated total tissue lead concentrations of 4, 96 and 430 µg/g, respectively. Subcellular fractionation indicated that around 70% of the total accumulated tissue lead was detoxified, three quarters of the detoxified lead fraction was converted into metal rich granules, with the remainder in the metallothione in like protein fraction. The majority of biologically active lead was associated with the mitochondrial fraction with up to a 128 fold increase in lead burden in exposed organisms compared to controls. This indicates lead detoxification was occurring but the organism was unable to prevent lead interacting with sensitive organelles. With increased lead exposure T. deltoidalis showed a suppression in glutathione peroxidase activity, total glutathione concentration and reduced GSH:GSSG ratios, however, these differences were not significant. Lead exposed T. deltoidalis had a significantly reduced total antioxidant capacity which corresponded with increased lipid peroxidation, lysosomal destabilisation and micronuclei frequency. The exposure–dose–response relationships demonstrated for lead exposed T. deltoidalis supports its potential for the development of sublethal endpoints in lead toxicity assessment.

    KW - Biomarkers

    KW - Subcellular lead

    KW - Oxidative stress

    KW - Lysosomes

    KW - Lipid peroxidation

    KW - Micronuclei

    KW - Bivalve.

    U2 - 10.1016/j.cbpc.2013.09.006

    DO - 10.1016/j.cbpc.2013.09.006

    M3 - Article

    VL - 159

    SP - 52

    EP - 61

    JO - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology

    JF - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology

    SN - 1532-0456

    ER -