Exposure of the freshwater bivalve Hyridella australis to metal contaminated sediments in the field and laboratory microcosms

metal uptake and effects

Chamani P.M. Marasinghe Wadige, Anne M. Taylor, Frank Krikowa, Mark Lintermans, William A. Maher

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Metal uptake and induced toxic effects on Hyridella australis were investigated by establishing 28 day exposure–dose–response relationships (EDR) of transplanted H. australis at four sites along a sediment metal contamination gradient in the mine affected Molonglo River, NSW. Laboratory exposure of this organism to the same sediments, collected from in situ sites, was run concurrently. Metal concentrations in whole organisms, individual tissues and sub-cellular tissue fractions were measured as organism metal dose. Total antioxidant capacity (TAOC), lipid peroxidation (MDA) and lysosomal membrane destabilisation (LMS) were measured as biological responses. H. australis accumulated significantly higher tissue zinc concentrations compared to the other metals. In situ organisms at the mine affected sites accumulated more metals than organisms in laboratory microcosms. Accumulated zinc, cadmium and the total metal concentrations in whole organism tissues reflected exposure–dose relationships. Sub-cellular analysis showed that most of the accumulated metals, both in the field and laboratory exposed organisms, were detoxified over 28 days exposure. Clear exposure and dose dependent responses of decreased TAOC and measurable increases in MDA and LMS with increased metal exposure and dose were evident in H. australis caged in the river. In contrast, a dose–response relationship was only evident for cadmium in laboratory exposed organisms. Organisms caged at mine affected sites showed stronger EDR relationships than those exposed in laboratory microcosms as they were exposed to additional sources of dissolved zinc and cadmium. Exposure in laboratory microcosms underestimated metal uptake and effects, thus assessment of metal contaminated sediments should be undertaken “in situ”.

    Original languageEnglish
    Pages (from-to)415-434
    Number of pages20
    JournalEcotoxicology
    Volume26
    Issue number3
    DOIs
    Publication statusPublished - 1 Apr 2017

    Fingerprint

    Bivalvia
    Fresh Water
    microcosm
    bivalve
    Sediments
    Metals
    metal
    sediment
    Cadmium
    Zinc
    cadmium
    zinc
    Tissue
    Rivers
    antioxidant
    exposure
    laboratory
    effect
    Antioxidants
    membrane

    Cite this

    @article{519e7ff916af44e3a6712476e806b329,
    title = "Exposure of the freshwater bivalve Hyridella australis to metal contaminated sediments in the field and laboratory microcosms: metal uptake and effects",
    abstract = "Metal uptake and induced toxic effects on Hyridella australis were investigated by establishing 28 day exposure–dose–response relationships (EDR) of transplanted H. australis at four sites along a sediment metal contamination gradient in the mine affected Molonglo River, NSW. Laboratory exposure of this organism to the same sediments, collected from in situ sites, was run concurrently. Metal concentrations in whole organisms, individual tissues and sub-cellular tissue fractions were measured as organism metal dose. Total antioxidant capacity (TAOC), lipid peroxidation (MDA) and lysosomal membrane destabilisation (LMS) were measured as biological responses. H. australis accumulated significantly higher tissue zinc concentrations compared to the other metals. In situ organisms at the mine affected sites accumulated more metals than organisms in laboratory microcosms. Accumulated zinc, cadmium and the total metal concentrations in whole organism tissues reflected exposure–dose relationships. Sub-cellular analysis showed that most of the accumulated metals, both in the field and laboratory exposed organisms, were detoxified over 28 days exposure. Clear exposure and dose dependent responses of decreased TAOC and measurable increases in MDA and LMS with increased metal exposure and dose were evident in H. australis caged in the river. In contrast, a dose–response relationship was only evident for cadmium in laboratory exposed organisms. Organisms caged at mine affected sites showed stronger EDR relationships than those exposed in laboratory microcosms as they were exposed to additional sources of dissolved zinc and cadmium. Exposure in laboratory microcosms underestimated metal uptake and effects, thus assessment of metal contaminated sediments should be undertaken “in situ”.",
    keywords = "Biomarkers, In situ, Oxidative stress, Sub-cellular partitioning, Transplanted bivalve",
    author = "{Marasinghe Wadige}, {Chamani P.M.} and Taylor, {Anne M.} and Frank Krikowa and Mark Lintermans and Maher, {William A.}",
    year = "2017",
    month = "4",
    day = "1",
    doi = "10.1007/s10646-017-1774-7",
    language = "English",
    volume = "26",
    pages = "415--434",
    journal = "Ecotoxicology",
    issn = "0963-9292",
    publisher = "Springer",
    number = "3",

    }

    Exposure of the freshwater bivalve Hyridella australis to metal contaminated sediments in the field and laboratory microcosms : metal uptake and effects. / Marasinghe Wadige, Chamani P.M.; Taylor, Anne M.; Krikowa, Frank; Lintermans, Mark; Maher, William A.

    In: Ecotoxicology, Vol. 26, No. 3, 01.04.2017, p. 415-434.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Exposure of the freshwater bivalve Hyridella australis to metal contaminated sediments in the field and laboratory microcosms

    T2 - metal uptake and effects

    AU - Marasinghe Wadige, Chamani P.M.

    AU - Taylor, Anne M.

    AU - Krikowa, Frank

    AU - Lintermans, Mark

    AU - Maher, William A.

    PY - 2017/4/1

    Y1 - 2017/4/1

    N2 - Metal uptake and induced toxic effects on Hyridella australis were investigated by establishing 28 day exposure–dose–response relationships (EDR) of transplanted H. australis at four sites along a sediment metal contamination gradient in the mine affected Molonglo River, NSW. Laboratory exposure of this organism to the same sediments, collected from in situ sites, was run concurrently. Metal concentrations in whole organisms, individual tissues and sub-cellular tissue fractions were measured as organism metal dose. Total antioxidant capacity (TAOC), lipid peroxidation (MDA) and lysosomal membrane destabilisation (LMS) were measured as biological responses. H. australis accumulated significantly higher tissue zinc concentrations compared to the other metals. In situ organisms at the mine affected sites accumulated more metals than organisms in laboratory microcosms. Accumulated zinc, cadmium and the total metal concentrations in whole organism tissues reflected exposure–dose relationships. Sub-cellular analysis showed that most of the accumulated metals, both in the field and laboratory exposed organisms, were detoxified over 28 days exposure. Clear exposure and dose dependent responses of decreased TAOC and measurable increases in MDA and LMS with increased metal exposure and dose were evident in H. australis caged in the river. In contrast, a dose–response relationship was only evident for cadmium in laboratory exposed organisms. Organisms caged at mine affected sites showed stronger EDR relationships than those exposed in laboratory microcosms as they were exposed to additional sources of dissolved zinc and cadmium. Exposure in laboratory microcosms underestimated metal uptake and effects, thus assessment of metal contaminated sediments should be undertaken “in situ”.

    AB - Metal uptake and induced toxic effects on Hyridella australis were investigated by establishing 28 day exposure–dose–response relationships (EDR) of transplanted H. australis at four sites along a sediment metal contamination gradient in the mine affected Molonglo River, NSW. Laboratory exposure of this organism to the same sediments, collected from in situ sites, was run concurrently. Metal concentrations in whole organisms, individual tissues and sub-cellular tissue fractions were measured as organism metal dose. Total antioxidant capacity (TAOC), lipid peroxidation (MDA) and lysosomal membrane destabilisation (LMS) were measured as biological responses. H. australis accumulated significantly higher tissue zinc concentrations compared to the other metals. In situ organisms at the mine affected sites accumulated more metals than organisms in laboratory microcosms. Accumulated zinc, cadmium and the total metal concentrations in whole organism tissues reflected exposure–dose relationships. Sub-cellular analysis showed that most of the accumulated metals, both in the field and laboratory exposed organisms, were detoxified over 28 days exposure. Clear exposure and dose dependent responses of decreased TAOC and measurable increases in MDA and LMS with increased metal exposure and dose were evident in H. australis caged in the river. In contrast, a dose–response relationship was only evident for cadmium in laboratory exposed organisms. Organisms caged at mine affected sites showed stronger EDR relationships than those exposed in laboratory microcosms as they were exposed to additional sources of dissolved zinc and cadmium. Exposure in laboratory microcosms underestimated metal uptake and effects, thus assessment of metal contaminated sediments should be undertaken “in situ”.

    KW - Biomarkers

    KW - In situ

    KW - Oxidative stress

    KW - Sub-cellular partitioning

    KW - Transplanted bivalve

    UR - http://www.scopus.com/inward/record.url?scp=85015825536&partnerID=8YFLogxK

    U2 - 10.1007/s10646-017-1774-7

    DO - 10.1007/s10646-017-1774-7

    M3 - Article

    VL - 26

    SP - 415

    EP - 434

    JO - Ecotoxicology

    JF - Ecotoxicology

    SN - 0963-9292

    IS - 3

    ER -