Expression and characterisation of the ovine respiratory syncytial virus (ORSV) G protein for use as a diagnostic reagent

R Ghildyal, A Chapman, I Peroulis, J Mills, J Meanger

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in children and calves. Antibodies to ovine RSV (ORSV) are common in sheep, but the clinical disease is not well defined. There is no report of ORSV infection in Australian sheep although respiratory distress syndrome has been described. This discrepancy may be due to the lack of a suitable diagnostic test. In this report, we have characterised the ORSV G protein in an attempt to study its relatedness to human and bovine RSV (HRSV, BRSV) and for use in the development of a suitable diagnostic assay. Full length and a truncated variant of ORSV G protein were expressed in mammalian cells and the expressed proteins characterised by indirect immunofluorescence and radioimmunoprecipitation assays. Our results indicate that like HRSV, the ORSV G protein is heavily glycosylated. The expressed protein was membrane bound as well as secreted and could be purified from culture supernatants and may be suitable for use in development of a diagnostic assay.

Original languageEnglish
Pages (from-to)475-482
Number of pages8
JournalVeterinary Research
Volume30
Issue number5
Publication statusPublished - 30 Oct 1999
Externally publishedYes

Fingerprint

Pneumovirus
Respiratory Syncytial Viruses
G-proteins
GTP-Binding Proteins
Sheep
assays
Human respiratory syncytial virus
sheep
Bovine respiratory syncytial virus
Sheep Diseases
Radioimmunoprecipitation Assay
Respiratory Syncytial Virus Infections
distress
diagnostic techniques
respiratory tract diseases
fluorescent antibody technique
Indirect Fluorescent Antibody Technique
Routine Diagnostic Tests
Respiratory Tract Infections
proteins

Cite this

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abstract = "Respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in children and calves. Antibodies to ovine RSV (ORSV) are common in sheep, but the clinical disease is not well defined. There is no report of ORSV infection in Australian sheep although respiratory distress syndrome has been described. This discrepancy may be due to the lack of a suitable diagnostic test. In this report, we have characterised the ORSV G protein in an attempt to study its relatedness to human and bovine RSV (HRSV, BRSV) and for use in the development of a suitable diagnostic assay. Full length and a truncated variant of ORSV G protein were expressed in mammalian cells and the expressed proteins characterised by indirect immunofluorescence and radioimmunoprecipitation assays. Our results indicate that like HRSV, the ORSV G protein is heavily glycosylated. The expressed protein was membrane bound as well as secreted and could be purified from culture supernatants and may be suitable for use in development of a diagnostic assay.",
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Expression and characterisation of the ovine respiratory syncytial virus (ORSV) G protein for use as a diagnostic reagent. / Ghildyal, R; Chapman, A; Peroulis, I; Mills, J; Meanger, J.

In: Veterinary Research, Vol. 30, No. 5, 30.10.1999, p. 475-482.

Research output: Contribution to journalArticle

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