Expression and partial characterisation of rabbit haemorrhagic disease virus non-structural proteins

Nadezda Urakova, Michael FRESE, Robyn Hall, June Liu, Klaus Matthaei, Tanja Strive

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    The intracellular replication and molecular virulence mechanisms of Rabbit haemorrhagic disease virus (RHDV) are poorly understood, mainly due to the lack of an effective cell culture system for this virus. To increase our understanding of RHDV molecular biology, the subcellular localisation of recombinant non-structural RHDV proteins was investigated in transiently transfected rabbit kidney (RK-13) cells. We provide evidence for oligomerisation of p23, and an ability of the viral protease to cleave the p16:p23 junction in trans, outside the context of the nascent polyprotein chain. Notably, expression of the viral polymerase alone and in the context of the entire RHDV polyprotein resulted in a redistribution of the Golgi network. This suggests that, similar to other positive-strand RNA viruses, RHDV may recruit membranes of the secretory pathway during replication, and that the viral polymerase may play a critical role during this process.
    Original languageEnglish
    Pages (from-to)69-79
    Number of pages11
    JournalVirology
    Volume484
    Issue number4
    DOIs
    Publication statusPublished - 1 Oct 2015

    Fingerprint

    Rabbit Haemorrhagic Disease Virus
    Viral Structural Proteins
    Polyproteins
    Secretory Pathway
    RNA Viruses
    Virulence
    Molecular Biology
    Peptide Hydrolases
    Cell Culture Techniques
    Rabbits
    Viruses
    Kidney
    Membranes

    Cite this

    Urakova, Nadezda ; FRESE, Michael ; Hall, Robyn ; Liu, June ; Matthaei, Klaus ; Strive, Tanja. / Expression and partial characterisation of rabbit haemorrhagic disease virus non-structural proteins. In: Virology. 2015 ; Vol. 484, No. 4. pp. 69-79.
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    Expression and partial characterisation of rabbit haemorrhagic disease virus non-structural proteins. / Urakova, Nadezda; FRESE, Michael; Hall, Robyn; Liu, June; Matthaei, Klaus; Strive, Tanja.

    In: Virology, Vol. 484, No. 4, 01.10.2015, p. 69-79.

    Research output: Contribution to journalArticle

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