Expression of c-Kit receptor mRNA and protein in the developing, adult and irradiated rodent testis

Sridurga Mithra Prabhu, Marvin L. Meistrich, Eileen A. McLaughlin, Shaun D. Roman, Sam Warne, Sirisha Mendis, Catherine Itman, Kate Lakoski Loveland

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Germ cell proliferation, migration and survival during all stages of spermatogenesis are affected by stem cell factor signalling through the c-Kit receptor, the expression and function of which are vital for normal male reproductive function. The present study comprehensively describes the c-Kit mRNA and protein cellular expression profiles in germ cells of the postnatal and adult rodent testis, revealing their significant elevation in synthesis at the onset of spermatogenesis. Real-time PCR analysis for both mice and rats matched the cellular mRNA expression profile where examined. Localization studies in normal mouse testes indicated that both c-Kit mRNA and protein are first detectable in differentiating spermatogonia. In addition, all spermatogonia isolated from 8-day-old mice displayed detectable c-Kit mRNA, but 30-50% of these lacked protein expression. The c-Kit mRNA and protein profile in normal rat testes indicated expression in gonocytes, in addition to differentiating spermatogonia. However, in the irradiated adult rat testes, in which undifferentiated spermatogonia are the only germ cell type, mRNA was also detected in the absence of protein. This persisted at 3 days and 1 and 2 weeks following treatment with gonadotrophin-releasing hormone (GnRH) antagonist to stimulate spermatogenesis recovery. By 4 weeks of GnRH antagonist treatment, accompanying the emergence of differentiating spermatogonia, both mRNA and protein were detected. Based on these observations, we propose that c-Kit mRNA and protein synthesis are regulated separately, possibly by influences linked to testis maturation and circulating hormone levels.

Original languageEnglish
Pages (from-to)489-499
Number of pages11
JournalReproduction
Volume131
Issue number3
DOIs
Publication statusPublished - Mar 2006
Externally publishedYes

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Proto-Oncogene Proteins c-kit
Testis
Rodentia
Spermatogonia
Messenger RNA
Spermatogenesis
Germ Cells
Hormone Antagonists
Gonadotropin-Releasing Hormone
Proteins
Stem Cell Factor
Cell Movement
Real-Time Polymerase Chain Reaction
Cell Survival
Cell Proliferation
Hormones

Cite this

Prabhu, S. M., Meistrich, M. L., McLaughlin, E. A., Roman, S. D., Warne, S., Mendis, S., ... Loveland, K. L. (2006). Expression of c-Kit receptor mRNA and protein in the developing, adult and irradiated rodent testis. Reproduction, 131(3), 489-499. https://doi.org/10.1530/rep.1.00968
Prabhu, Sridurga Mithra ; Meistrich, Marvin L. ; McLaughlin, Eileen A. ; Roman, Shaun D. ; Warne, Sam ; Mendis, Sirisha ; Itman, Catherine ; Loveland, Kate Lakoski. / Expression of c-Kit receptor mRNA and protein in the developing, adult and irradiated rodent testis. In: Reproduction. 2006 ; Vol. 131, No. 3. pp. 489-499.
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Prabhu, SM, Meistrich, ML, McLaughlin, EA, Roman, SD, Warne, S, Mendis, S, Itman, C & Loveland, KL 2006, 'Expression of c-Kit receptor mRNA and protein in the developing, adult and irradiated rodent testis', Reproduction, vol. 131, no. 3, pp. 489-499. https://doi.org/10.1530/rep.1.00968

Expression of c-Kit receptor mRNA and protein in the developing, adult and irradiated rodent testis. / Prabhu, Sridurga Mithra; Meistrich, Marvin L.; McLaughlin, Eileen A.; Roman, Shaun D.; Warne, Sam; Mendis, Sirisha; Itman, Catherine; Loveland, Kate Lakoski.

In: Reproduction, Vol. 131, No. 3, 03.2006, p. 489-499.

Research output: Contribution to journalArticle

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AU - Prabhu, Sridurga Mithra

AU - Meistrich, Marvin L.

AU - McLaughlin, Eileen A.

AU - Roman, Shaun D.

AU - Warne, Sam

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AU - Itman, Catherine

AU - Loveland, Kate Lakoski

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AB - Germ cell proliferation, migration and survival during all stages of spermatogenesis are affected by stem cell factor signalling through the c-Kit receptor, the expression and function of which are vital for normal male reproductive function. The present study comprehensively describes the c-Kit mRNA and protein cellular expression profiles in germ cells of the postnatal and adult rodent testis, revealing their significant elevation in synthesis at the onset of spermatogenesis. Real-time PCR analysis for both mice and rats matched the cellular mRNA expression profile where examined. Localization studies in normal mouse testes indicated that both c-Kit mRNA and protein are first detectable in differentiating spermatogonia. In addition, all spermatogonia isolated from 8-day-old mice displayed detectable c-Kit mRNA, but 30-50% of these lacked protein expression. The c-Kit mRNA and protein profile in normal rat testes indicated expression in gonocytes, in addition to differentiating spermatogonia. However, in the irradiated adult rat testes, in which undifferentiated spermatogonia are the only germ cell type, mRNA was also detected in the absence of protein. This persisted at 3 days and 1 and 2 weeks following treatment with gonadotrophin-releasing hormone (GnRH) antagonist to stimulate spermatogenesis recovery. By 4 weeks of GnRH antagonist treatment, accompanying the emergence of differentiating spermatogonia, both mRNA and protein were detected. Based on these observations, we propose that c-Kit mRNA and protein synthesis are regulated separately, possibly by influences linked to testis maturation and circulating hormone levels.

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