Expression of rabbit IL-4 by recombinant myxoma viruses enhances virulence and overcomes genetic resistance to myxomatosis

P. J. Kerr, H. D. Perkins, B. Inglis, R. Stagg, E. McLaughlin, S. V. Collins, B. H. Van Leeuwen

Research output: Contribution to journalArticle

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Abstract

Rabbit IL-4 was expressed in the virulent standard laboratory strain (SLS) and the attenuated Uriarra (Ur) strain of myxoma virus with the aim of creating a Th2 cytokine environment and inhibiting the development of an antiviral cell-mediated response to myxomatosis in infected rabbits. This allowed testing of a model for genetic resistance to myxomatosis in wild rabbits that have undergone 50 years of natural selection for resistance to myxomatosis. Expression of IL-4 significantly enhanced virulence of both virulent and attenuated virus strains in susceptible (laboratory) and resistant (wild) rabbits. SLS-IL-4 completely overcame genetic resistance in wild rabbits. The pathogenesis of SLS-IL-4 was compared in susceptible and resistant rabbits. The results support a model for resistance to myxomatosis of an enhanced innate immune response controlling virus replication and allowing an effective antiviral cell-mediated immune response to develop in resistant rabbits. Expression of IL-4 did not overcome immunity to myxomatosis induced by immunization.

Original languageEnglish
Pages (from-to)117-128
Number of pages12
JournalVirology
Volume324
Issue number1
DOIs
Publication statusPublished - 20 Jun 2004
Externally publishedYes

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Myxoma virus
Interleukin-4
Virulence
Rabbits
Innate Immunity
Antiviral Agents
Genetic Selection
Genetic Models
Virus Replication
Immunity
Immunization
Cytokines
Viruses

Cite this

Kerr, P. J., Perkins, H. D., Inglis, B., Stagg, R., McLaughlin, E., Collins, S. V., & Van Leeuwen, B. H. (2004). Expression of rabbit IL-4 by recombinant myxoma viruses enhances virulence and overcomes genetic resistance to myxomatosis. Virology, 324(1), 117-128. https://doi.org/10.1016/j.virol.2004.02.031
Kerr, P. J. ; Perkins, H. D. ; Inglis, B. ; Stagg, R. ; McLaughlin, E. ; Collins, S. V. ; Van Leeuwen, B. H. / Expression of rabbit IL-4 by recombinant myxoma viruses enhances virulence and overcomes genetic resistance to myxomatosis. In: Virology. 2004 ; Vol. 324, No. 1. pp. 117-128.
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Kerr, PJ, Perkins, HD, Inglis, B, Stagg, R, McLaughlin, E, Collins, SV & Van Leeuwen, BH 2004, 'Expression of rabbit IL-4 by recombinant myxoma viruses enhances virulence and overcomes genetic resistance to myxomatosis', Virology, vol. 324, no. 1, pp. 117-128. https://doi.org/10.1016/j.virol.2004.02.031

Expression of rabbit IL-4 by recombinant myxoma viruses enhances virulence and overcomes genetic resistance to myxomatosis. / Kerr, P. J.; Perkins, H. D.; Inglis, B.; Stagg, R.; McLaughlin, E.; Collins, S. V.; Van Leeuwen, B. H.

In: Virology, Vol. 324, No. 1, 20.06.2004, p. 117-128.

Research output: Contribution to journalArticle

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AU - Kerr, P. J.

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AU - McLaughlin, E.

AU - Collins, S. V.

AU - Van Leeuwen, B. H.

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