Factors influencing the post-exercise hepcidin-25 response in elite athletes

Peter Peeling, Alannah K A McKay, David B Pyne, Kym J Guelfi, Rachel H McCormick, Coby M Laarakkers, Dorine W Swinkels, Laura A Garvican-Lewis, Megan L R Ross, Avish P Sharma, Jill J Leckey, Louise M Burke

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    Abstract

    PURPOSE: The extent to which hepcidin regulation after acute bouts of exercise is influenced by baseline (resting) concentrations of key iron parameters remains uncertain. This investigation explored the influence of selected iron parameters and 25-km race walk time on 3-h post-exercise hepcidin-25 levels in international-level race walkers.

    METHODS: Twenty-four male race walkers completed a graded exercise test and a 25-km race-walk trial. Throughout the 25-km race-walk, venous blood samples were collected pre-exercise, immediately post-exercise, and at 3-h post-exercise. Blood was analysed for serum ferritin, serum iron, Interleukin-6 (IL-6), and hepcidin-25 concentration.

    RESULTS: IL-6 and hepcidin-25 increased (7.6- and 7.5-fold, respectively) in response to the 25-km race-walk trial (both p < 0.01). Significant individual relationships were evident between 3-h post-exercise hepcidin-25, baseline serum ferritin and serum iron (r > 0.62; p < 0.05). Multiple regression analysis showed that these two iron parameters, in addition to post-exercise IL-6 concentration and 25-km race-walk time, accounted for ~77% of the variance in 3-h post-exercise hepcidin-25 (p < 0.01). A median split by the cohort's baseline serum ferritin concentration (LOW: 58.0 vs. HIGH: 101.8 µg/L; p < 0.01) showed a significant between group difference in the 3-h post-exercise hepcidin-25 (LOW: 6.0 ± 3.6 vs. 11.3 ± 5.4 nM; p = 0.01), despite no differences in baseline serum iron, post-exercise IL-6, or 25-km race-walk time (all p > 0.05).

    CONCLUSION: Despite exercise activating numerous hepcidin regulators, baseline iron status appears to play a dominant role in the regulation of hepcidin-25 in elite-level athletes subsequent to endurance exercise.

    Original languageEnglish
    Pages (from-to)1233-1239
    Number of pages7
    JournalEuropean Journal of Applied Physiology
    Volume117
    Issue number6
    DOIs
    Publication statusPublished - Jun 2017

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    Hepcidins
    Athletes
    Exercise
    Iron
    Walkers
    Interleukin-6
    Ferritins
    Serum
    Exercise Test

    Cite this

    Peeling, P., McKay, A. K. A., Pyne, D. B., Guelfi, K. J., McCormick, R. H., Laarakkers, C. M., ... Burke, L. M. (2017). Factors influencing the post-exercise hepcidin-25 response in elite athletes. European Journal of Applied Physiology, 117(6), 1233-1239. https://doi.org/10.1007/s00421-017-3611-3
    Peeling, Peter ; McKay, Alannah K A ; Pyne, David B ; Guelfi, Kym J ; McCormick, Rachel H ; Laarakkers, Coby M ; Swinkels, Dorine W ; Garvican-Lewis, Laura A ; Ross, Megan L R ; Sharma, Avish P ; Leckey, Jill J ; Burke, Louise M. / Factors influencing the post-exercise hepcidin-25 response in elite athletes. In: European Journal of Applied Physiology. 2017 ; Vol. 117, No. 6. pp. 1233-1239.
    @article{e72dd3211ece4a2f97a21312ffc29567,
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    abstract = "PURPOSE: The extent to which hepcidin regulation after acute bouts of exercise is influenced by baseline (resting) concentrations of key iron parameters remains uncertain. This investigation explored the influence of selected iron parameters and 25-km race walk time on 3-h post-exercise hepcidin-25 levels in international-level race walkers.METHODS: Twenty-four male race walkers completed a graded exercise test and a 25-km race-walk trial. Throughout the 25-km race-walk, venous blood samples were collected pre-exercise, immediately post-exercise, and at 3-h post-exercise. Blood was analysed for serum ferritin, serum iron, Interleukin-6 (IL-6), and hepcidin-25 concentration.RESULTS: IL-6 and hepcidin-25 increased (7.6- and 7.5-fold, respectively) in response to the 25-km race-walk trial (both p < 0.01). Significant individual relationships were evident between 3-h post-exercise hepcidin-25, baseline serum ferritin and serum iron (r > 0.62; p < 0.05). Multiple regression analysis showed that these two iron parameters, in addition to post-exercise IL-6 concentration and 25-km race-walk time, accounted for ~77{\%} of the variance in 3-h post-exercise hepcidin-25 (p < 0.01). A median split by the cohort's baseline serum ferritin concentration (LOW: 58.0 vs. HIGH: 101.8 µg/L; p < 0.01) showed a significant between group difference in the 3-h post-exercise hepcidin-25 (LOW: 6.0 ± 3.6 vs. 11.3 ± 5.4 nM; p = 0.01), despite no differences in baseline serum iron, post-exercise IL-6, or 25-km race-walk time (all p > 0.05).CONCLUSION: Despite exercise activating numerous hepcidin regulators, baseline iron status appears to play a dominant role in the regulation of hepcidin-25 in elite-level athletes subsequent to endurance exercise.",
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    author = "Peter Peeling and McKay, {Alannah K A} and Pyne, {David B} and Guelfi, {Kym J} and McCormick, {Rachel H} and Laarakkers, {Coby M} and Swinkels, {Dorine W} and Garvican-Lewis, {Laura A} and Ross, {Megan L R} and Sharma, {Avish P} and Leckey, {Jill J} and Burke, {Louise M}",
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    Peeling, P, McKay, AKA, Pyne, DB, Guelfi, KJ, McCormick, RH, Laarakkers, CM, Swinkels, DW, Garvican-Lewis, LA, Ross, MLR, Sharma, AP, Leckey, JJ & Burke, LM 2017, 'Factors influencing the post-exercise hepcidin-25 response in elite athletes', European Journal of Applied Physiology, vol. 117, no. 6, pp. 1233-1239. https://doi.org/10.1007/s00421-017-3611-3

    Factors influencing the post-exercise hepcidin-25 response in elite athletes. / Peeling, Peter; McKay, Alannah K A; Pyne, David B; Guelfi, Kym J; McCormick, Rachel H; Laarakkers, Coby M; Swinkels, Dorine W; Garvican-Lewis, Laura A; Ross, Megan L R; Sharma, Avish P; Leckey, Jill J; Burke, Louise M.

    In: European Journal of Applied Physiology, Vol. 117, No. 6, 06.2017, p. 1233-1239.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Factors influencing the post-exercise hepcidin-25 response in elite athletes

    AU - Peeling, Peter

    AU - McKay, Alannah K A

    AU - Pyne, David B

    AU - Guelfi, Kym J

    AU - McCormick, Rachel H

    AU - Laarakkers, Coby M

    AU - Swinkels, Dorine W

    AU - Garvican-Lewis, Laura A

    AU - Ross, Megan L R

    AU - Sharma, Avish P

    AU - Leckey, Jill J

    AU - Burke, Louise M

    PY - 2017/6

    Y1 - 2017/6

    N2 - PURPOSE: The extent to which hepcidin regulation after acute bouts of exercise is influenced by baseline (resting) concentrations of key iron parameters remains uncertain. This investigation explored the influence of selected iron parameters and 25-km race walk time on 3-h post-exercise hepcidin-25 levels in international-level race walkers.METHODS: Twenty-four male race walkers completed a graded exercise test and a 25-km race-walk trial. Throughout the 25-km race-walk, venous blood samples were collected pre-exercise, immediately post-exercise, and at 3-h post-exercise. Blood was analysed for serum ferritin, serum iron, Interleukin-6 (IL-6), and hepcidin-25 concentration.RESULTS: IL-6 and hepcidin-25 increased (7.6- and 7.5-fold, respectively) in response to the 25-km race-walk trial (both p < 0.01). Significant individual relationships were evident between 3-h post-exercise hepcidin-25, baseline serum ferritin and serum iron (r > 0.62; p < 0.05). Multiple regression analysis showed that these two iron parameters, in addition to post-exercise IL-6 concentration and 25-km race-walk time, accounted for ~77% of the variance in 3-h post-exercise hepcidin-25 (p < 0.01). A median split by the cohort's baseline serum ferritin concentration (LOW: 58.0 vs. HIGH: 101.8 µg/L; p < 0.01) showed a significant between group difference in the 3-h post-exercise hepcidin-25 (LOW: 6.0 ± 3.6 vs. 11.3 ± 5.4 nM; p = 0.01), despite no differences in baseline serum iron, post-exercise IL-6, or 25-km race-walk time (all p > 0.05).CONCLUSION: Despite exercise activating numerous hepcidin regulators, baseline iron status appears to play a dominant role in the regulation of hepcidin-25 in elite-level athletes subsequent to endurance exercise.

    AB - PURPOSE: The extent to which hepcidin regulation after acute bouts of exercise is influenced by baseline (resting) concentrations of key iron parameters remains uncertain. This investigation explored the influence of selected iron parameters and 25-km race walk time on 3-h post-exercise hepcidin-25 levels in international-level race walkers.METHODS: Twenty-four male race walkers completed a graded exercise test and a 25-km race-walk trial. Throughout the 25-km race-walk, venous blood samples were collected pre-exercise, immediately post-exercise, and at 3-h post-exercise. Blood was analysed for serum ferritin, serum iron, Interleukin-6 (IL-6), and hepcidin-25 concentration.RESULTS: IL-6 and hepcidin-25 increased (7.6- and 7.5-fold, respectively) in response to the 25-km race-walk trial (both p < 0.01). Significant individual relationships were evident between 3-h post-exercise hepcidin-25, baseline serum ferritin and serum iron (r > 0.62; p < 0.05). Multiple regression analysis showed that these two iron parameters, in addition to post-exercise IL-6 concentration and 25-km race-walk time, accounted for ~77% of the variance in 3-h post-exercise hepcidin-25 (p < 0.01). A median split by the cohort's baseline serum ferritin concentration (LOW: 58.0 vs. HIGH: 101.8 µg/L; p < 0.01) showed a significant between group difference in the 3-h post-exercise hepcidin-25 (LOW: 6.0 ± 3.6 vs. 11.3 ± 5.4 nM; p = 0.01), despite no differences in baseline serum iron, post-exercise IL-6, or 25-km race-walk time (all p > 0.05).CONCLUSION: Despite exercise activating numerous hepcidin regulators, baseline iron status appears to play a dominant role in the regulation of hepcidin-25 in elite-level athletes subsequent to endurance exercise.

    KW - Journal Article

    U2 - 10.1007/s00421-017-3611-3

    DO - 10.1007/s00421-017-3611-3

    M3 - Article

    VL - 117

    SP - 1233

    EP - 1239

    JO - European Journal of Applied Physiology and Occupational Physiology

    JF - European Journal of Applied Physiology and Occupational Physiology

    SN - 1439-6319

    IS - 6

    ER -