First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes):

insights into the karyotype evolution of this ancient fish order

Terumi Hatanaka, Ezequiel Aguiar De Oliveira, Petr Ráb, Cassia Fernanda Yano, Luiz Antônio Carlos Bertollo, Tariq EZAZ, Oladele OI Jegedi, Thomas Liehr, Victor F Olaleye, Marcelo DB Cioffi

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Osteoglossiformes is one of the most ancient (Early Triassic) groups of freshwater teleosts and has at least one representative on each continent of the Southern Hemisphere, with the exception of Antarctica. Among its six described families, the Gymnarchidae occurs in many African rivers, including the river Nile, where Gymnarchus niloticus is its only representative. In this study we provide, for the first time, a comprehensive cytogenetic analysis for G. niloticus, which includes definition of the standard karyotype, C-banding, repetitive DNA mapping and comparative genomic hybridization, in order to investigate its chromosomal patterns and provide new data for comparative analyses with other Osteoglossiformes species. The 2n was 34 (26 metacentric + 8 submetacentric) in both sexes, and C-positive heterochromatic bands were detected in the centromeric/pericentromeric regions of all chromosomes. The simultaneous detection of 18S and 5S ribosomal DNAs showed simple and non-syntenical locations for such sequences, and a Chromomycin A3-positive band was observed, which is co-localized within the 18S ribosomal RNA gene sequences. Fluorescence in situ hybridization using U2 small nuclear RNA, Rex6 and some microssatelites as probes showed a scattered distribution among chromosomes. Data from comparative genomic hybridization experiments with some other African Osteoglossiformes species showed that all these species have an advanced stage of sequence divergence. The results highlight the evolutionary diversification of osteoglossiform fishes.
    Original languageEnglish
    Pages (from-to)83-92
    Number of pages10
    JournalBiological Journal of the Linnean Society
    Volume125
    Issue number1
    DOIs
    Publication statusPublished - 20 Jul 2018

    Fingerprint

    Osteoglossiformes
    karyotype
    karyotyping
    comparative genomic hybridization
    RNA
    chromosome
    genomics
    fish
    small nuclear RNA
    Nile River
    chromosomes
    DNA
    cytogenetic analysis
    cytogenetics
    chromosome banding
    fluorescence in situ hybridization
    teleost
    river
    ribosomal DNA
    Antarctica

    Cite this

    Hatanaka, Terumi ; De Oliveira, Ezequiel Aguiar ; Ráb, Petr ; Yano, Cassia Fernanda ; Bertollo, Luiz Antônio Carlos ; EZAZ, Tariq ; Jegedi, Oladele OI ; Liehr, Thomas ; Olaleye, Victor F ; Cioffi, Marcelo DB. / First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes): insights into the karyotype evolution of this ancient fish order. In: Biological Journal of the Linnean Society. 2018 ; Vol. 125, No. 1. pp. 83-92.
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    abstract = "Osteoglossiformes is one of the most ancient (Early Triassic) groups of freshwater teleosts and has at least one representative on each continent of the Southern Hemisphere, with the exception of Antarctica. Among its six described families, the Gymnarchidae occurs in many African rivers, including the river Nile, where Gymnarchus niloticus is its only representative. In this study we provide, for the first time, a comprehensive cytogenetic analysis for G. niloticus, which includes definition of the standard karyotype, C-banding, repetitive DNA mapping and comparative genomic hybridization, in order to investigate its chromosomal patterns and provide new data for comparative analyses with other Osteoglossiformes species. The 2n was 34 (26 metacentric + 8 submetacentric) in both sexes, and C-positive heterochromatic bands were detected in the centromeric/pericentromeric regions of all chromosomes. The simultaneous detection of 18S and 5S ribosomal DNAs showed simple and non-syntenical locations for such sequences, and a Chromomycin A3-positive band was observed, which is co-localized within the 18S ribosomal RNA gene sequences. Fluorescence in situ hybridization using U2 small nuclear RNA, Rex6 and some microssatelites as probes showed a scattered distribution among chromosomes. Data from comparative genomic hybridization experiments with some other African Osteoglossiformes species showed that all these species have an advanced stage of sequence divergence. The results highlight the evolutionary diversification of osteoglossiform fishes.",
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    First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes): insights into the karyotype evolution of this ancient fish order. / Hatanaka, Terumi; De Oliveira, Ezequiel Aguiar; Ráb, Petr; Yano, Cassia Fernanda; Bertollo, Luiz Antônio Carlos; EZAZ, Tariq; Jegedi, Oladele OI; Liehr, Thomas; Olaleye, Victor F; Cioffi, Marcelo DB.

    In: Biological Journal of the Linnean Society, Vol. 125, No. 1, 20.07.2018, p. 83-92.

    Research output: Contribution to journalArticle

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    T1 - First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes):

    T2 - insights into the karyotype evolution of this ancient fish order

    AU - Hatanaka, Terumi

    AU - De Oliveira, Ezequiel Aguiar

    AU - Ráb, Petr

    AU - Yano, Cassia Fernanda

    AU - Bertollo, Luiz Antônio Carlos

    AU - EZAZ, Tariq

    AU - Jegedi, Oladele OI

    AU - Liehr, Thomas

    AU - Olaleye, Victor F

    AU - Cioffi, Marcelo DB

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    Y1 - 2018/7/20

    N2 - Osteoglossiformes is one of the most ancient (Early Triassic) groups of freshwater teleosts and has at least one representative on each continent of the Southern Hemisphere, with the exception of Antarctica. Among its six described families, the Gymnarchidae occurs in many African rivers, including the river Nile, where Gymnarchus niloticus is its only representative. In this study we provide, for the first time, a comprehensive cytogenetic analysis for G. niloticus, which includes definition of the standard karyotype, C-banding, repetitive DNA mapping and comparative genomic hybridization, in order to investigate its chromosomal patterns and provide new data for comparative analyses with other Osteoglossiformes species. The 2n was 34 (26 metacentric + 8 submetacentric) in both sexes, and C-positive heterochromatic bands were detected in the centromeric/pericentromeric regions of all chromosomes. The simultaneous detection of 18S and 5S ribosomal DNAs showed simple and non-syntenical locations for such sequences, and a Chromomycin A3-positive band was observed, which is co-localized within the 18S ribosomal RNA gene sequences. Fluorescence in situ hybridization using U2 small nuclear RNA, Rex6 and some microssatelites as probes showed a scattered distribution among chromosomes. Data from comparative genomic hybridization experiments with some other African Osteoglossiformes species showed that all these species have an advanced stage of sequence divergence. The results highlight the evolutionary diversification of osteoglossiform fishes.

    AB - Osteoglossiformes is one of the most ancient (Early Triassic) groups of freshwater teleosts and has at least one representative on each continent of the Southern Hemisphere, with the exception of Antarctica. Among its six described families, the Gymnarchidae occurs in many African rivers, including the river Nile, where Gymnarchus niloticus is its only representative. In this study we provide, for the first time, a comprehensive cytogenetic analysis for G. niloticus, which includes definition of the standard karyotype, C-banding, repetitive DNA mapping and comparative genomic hybridization, in order to investigate its chromosomal patterns and provide new data for comparative analyses with other Osteoglossiformes species. The 2n was 34 (26 metacentric + 8 submetacentric) in both sexes, and C-positive heterochromatic bands were detected in the centromeric/pericentromeric regions of all chromosomes. The simultaneous detection of 18S and 5S ribosomal DNAs showed simple and non-syntenical locations for such sequences, and a Chromomycin A3-positive band was observed, which is co-localized within the 18S ribosomal RNA gene sequences. Fluorescence in situ hybridization using U2 small nuclear RNA, Rex6 and some microssatelites as probes showed a scattered distribution among chromosomes. Data from comparative genomic hybridization experiments with some other African Osteoglossiformes species showed that all these species have an advanced stage of sequence divergence. The results highlight the evolutionary diversification of osteoglossiform fishes.

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    KW - Chromosome banding

    KW - Comparative genomic hybrydization

    KW - Karyotype evolution

    KW - Molecular cytogenetics

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