Forensically relevant SNaPshot assays for human DNA SNP analysis: a review

Bhavik MEHTA, Runa Daniel, Chris Phillips, Dennis MCNEVIN

Research output: Contribution to journalReview article

21 Citations (Scopus)

Abstract

Short tandem repeats are the gold standard for human identification but are not informative for forensic DNA phenotyping (FDP). Single-nucleotide polymorphisms (SNPs) as genetic markers can be applied to both identification and FDP. The concept of DNA intelligence emerged with the potential for SNPs to infer biogeographical ancestry (BGA) and externally visible characteristics (EVCs), which together enable the FDP process. For more than a decade, the SNaPshot® technique has been utilised to analyse identity and FDP-associated SNPs in forensic DNA analysis. SNaPshot is a single-base extension (SBE) assay with capillary electrophoresis as its detection system. This multiplexing technique offers the advantage of easy integration into operational forensic laboratories without the requirement for any additional equipment. Further, the SNP panels from SNaPshot® assays can be incorporated into customised panels for massively parallel sequencing (MPS). Many SNaPshot® assays are available for identity, BGA and EVC profiling with examples including the well-known SNPforID 52-plex identity assay, the SNPforID 34-plex BGA assay and the HIrisPlex EVC assay. This review lists the major forensically relevant SNaPshot® assays for human DNA SNP analysis and can be used as a guide for selecting the appropriate assay for specific identity and FDP applications.
Original languageEnglish
Pages (from-to)21-37
Number of pages17
JournalInternational Journal of Legal Medicine
Volume131
Issue number1
DOIs
Publication statusPublished - Jan 2017

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Single Nucleotide Polymorphism
DNA
High-Throughput Nucleotide Sequencing
Forensic Anthropology
Capillary Electrophoresis
Intelligence
Genetic Markers
Microsatellite Repeats
Equipment and Supplies

Cite this

MEHTA, Bhavik ; Daniel, Runa ; Phillips, Chris ; MCNEVIN, Dennis. / Forensically relevant SNaPshot assays for human DNA SNP analysis: a review. In: International Journal of Legal Medicine. 2017 ; Vol. 131, No. 1. pp. 21-37.
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abstract = "Short tandem repeats are the gold standard for human identification but are not informative for forensic DNA phenotyping (FDP). Single-nucleotide polymorphisms (SNPs) as genetic markers can be applied to both identification and FDP. The concept of DNA intelligence emerged with the potential for SNPs to infer biogeographical ancestry (BGA) and externally visible characteristics (EVCs), which together enable the FDP process. For more than a decade, the SNaPshot{\circledR} technique has been utilised to analyse identity and FDP-associated SNPs in forensic DNA analysis. SNaPshot is a single-base extension (SBE) assay with capillary electrophoresis as its detection system. This multiplexing technique offers the advantage of easy integration into operational forensic laboratories without the requirement for any additional equipment. Further, the SNP panels from SNaPshot{\circledR} assays can be incorporated into customised panels for massively parallel sequencing (MPS). Many SNaPshot{\circledR} assays are available for identity, BGA and EVC profiling with examples including the well-known SNPforID 52-plex identity assay, the SNPforID 34-plex BGA assay and the HIrisPlex EVC assay. This review lists the major forensically relevant SNaPshot{\circledR} assays for human DNA SNP analysis and can be used as a guide for selecting the appropriate assay for specific identity and FDP applications.",
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Forensically relevant SNaPshot assays for human DNA SNP analysis: a review. / MEHTA, Bhavik; Daniel, Runa; Phillips, Chris; MCNEVIN, Dennis.

In: International Journal of Legal Medicine, Vol. 131, No. 1, 01.2017, p. 21-37.

Research output: Contribution to journalReview article

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