G80A reduced folate carrier SNP influences the absorption and cellular translocation of dietary folate and its association with blood pressure in an elderly population

Lisa Dufficy, Nenad Naumovski, Xiaowei Ng, Barbara Blades, Zoe Yates, Cheryl Travers, Peter Lewis, Jonathon Sturm, Martin Veysey, Paul D. Roach, Mark D. Lucock

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The functional consequences of the G80A RFC SNP on the expressed reduced folate carrier protein were evaluated by looking at the relationship between intake of folate, plasma folate and cellular stores of the vitamin. The effect on homocysteine was also examined. Homocysteine is a thiol that is known to be inversely associated with folate, and which is considered to be both thrombo- and athrogenic. At high levels, homocysteine may also interfere with nitric oxide mediated vasodilation, cause oxidative injury to, and proliferation of the vascular endothelium, and alter the elastic properties of the vascular wall, contributing to increased blood pressure. Participants (119; 52 male, 67 female) from a NSW retirement village were assessed. Independent of gender, the assimilation of folate from dietary sources into red cells showed a significant association for GG (r = 0.399; p = 0.022) and GA (r = 0.564; p < 0.0001) subjects, but not homozygous recessive (AA) individuals (r = 0.223; p = 0.236). The same genotype based pattern of significance was shown for the association between dietary folate and plasma folate (GG: r = 0.524; p = 0.002, GA: r = 0.408; p = 0.002). No genotype-related pattern of significance was shown for the association between dietary folate and homocysteine. When examined by gender, some differences were apparent; one-way ANOVA showed that genotype influenced diastolic blood pressure in males (p = 0.019), while only females showed a significant correlation between dietary folate and blood pressure within specific genotypes (Systolic pressure GA: r = - 0.372; p = 0.025, carriage of A: r = 0.-0.357; p = 0.011. Diastolic pressure GA: r = - 0.355; p = 0.034, carriage of A: r = 0.-0.310; p = 0.029). The G80A RFC SNP had an impact on the absorption and cellular translocation of dietary folate and its association with blood pressure in an elderly population.

Original languageEnglish
Pages (from-to)957-966
Number of pages10
JournalLife Sciences
Volume79
Issue number10
DOIs
Publication statusPublished - 1 Aug 2006
Externally publishedYes

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Reduced Folate Carrier Protein
Blood pressure
Folic Acid
Single Nucleotide Polymorphism
Blood Pressure
Homocysteine
Population
Genotype
Plasmas
Retirement
Vascular Endothelium
Analysis of variance (ANOVA)
Sulfhydryl Compounds
Vasodilation
Vitamins
Blood Vessels
Analysis of Variance
Nitric Oxide
Cells

Cite this

Dufficy, Lisa ; Naumovski, Nenad ; Ng, Xiaowei ; Blades, Barbara ; Yates, Zoe ; Travers, Cheryl ; Lewis, Peter ; Sturm, Jonathon ; Veysey, Martin ; Roach, Paul D. ; Lucock, Mark D. / G80A reduced folate carrier SNP influences the absorption and cellular translocation of dietary folate and its association with blood pressure in an elderly population. In: Life Sciences. 2006 ; Vol. 79, No. 10. pp. 957-966.
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G80A reduced folate carrier SNP influences the absorption and cellular translocation of dietary folate and its association with blood pressure in an elderly population. / Dufficy, Lisa; Naumovski, Nenad; Ng, Xiaowei; Blades, Barbara; Yates, Zoe; Travers, Cheryl; Lewis, Peter; Sturm, Jonathon; Veysey, Martin; Roach, Paul D.; Lucock, Mark D.

In: Life Sciences, Vol. 79, No. 10, 01.08.2006, p. 957-966.

Research output: Contribution to journalArticle

TY - JOUR

T1 - G80A reduced folate carrier SNP influences the absorption and cellular translocation of dietary folate and its association with blood pressure in an elderly population

AU - Dufficy, Lisa

AU - Naumovski, Nenad

AU - Ng, Xiaowei

AU - Blades, Barbara

AU - Yates, Zoe

AU - Travers, Cheryl

AU - Lewis, Peter

AU - Sturm, Jonathon

AU - Veysey, Martin

AU - Roach, Paul D.

AU - Lucock, Mark D.

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N2 - The functional consequences of the G80A RFC SNP on the expressed reduced folate carrier protein were evaluated by looking at the relationship between intake of folate, plasma folate and cellular stores of the vitamin. The effect on homocysteine was also examined. Homocysteine is a thiol that is known to be inversely associated with folate, and which is considered to be both thrombo- and athrogenic. At high levels, homocysteine may also interfere with nitric oxide mediated vasodilation, cause oxidative injury to, and proliferation of the vascular endothelium, and alter the elastic properties of the vascular wall, contributing to increased blood pressure. Participants (119; 52 male, 67 female) from a NSW retirement village were assessed. Independent of gender, the assimilation of folate from dietary sources into red cells showed a significant association for GG (r = 0.399; p = 0.022) and GA (r = 0.564; p < 0.0001) subjects, but not homozygous recessive (AA) individuals (r = 0.223; p = 0.236). The same genotype based pattern of significance was shown for the association between dietary folate and plasma folate (GG: r = 0.524; p = 0.002, GA: r = 0.408; p = 0.002). No genotype-related pattern of significance was shown for the association between dietary folate and homocysteine. When examined by gender, some differences were apparent; one-way ANOVA showed that genotype influenced diastolic blood pressure in males (p = 0.019), while only females showed a significant correlation between dietary folate and blood pressure within specific genotypes (Systolic pressure GA: r = - 0.372; p = 0.025, carriage of A: r = 0.-0.357; p = 0.011. Diastolic pressure GA: r = - 0.355; p = 0.034, carriage of A: r = 0.-0.310; p = 0.029). The G80A RFC SNP had an impact on the absorption and cellular translocation of dietary folate and its association with blood pressure in an elderly population.

AB - The functional consequences of the G80A RFC SNP on the expressed reduced folate carrier protein were evaluated by looking at the relationship between intake of folate, plasma folate and cellular stores of the vitamin. The effect on homocysteine was also examined. Homocysteine is a thiol that is known to be inversely associated with folate, and which is considered to be both thrombo- and athrogenic. At high levels, homocysteine may also interfere with nitric oxide mediated vasodilation, cause oxidative injury to, and proliferation of the vascular endothelium, and alter the elastic properties of the vascular wall, contributing to increased blood pressure. Participants (119; 52 male, 67 female) from a NSW retirement village were assessed. Independent of gender, the assimilation of folate from dietary sources into red cells showed a significant association for GG (r = 0.399; p = 0.022) and GA (r = 0.564; p < 0.0001) subjects, but not homozygous recessive (AA) individuals (r = 0.223; p = 0.236). The same genotype based pattern of significance was shown for the association between dietary folate and plasma folate (GG: r = 0.524; p = 0.002, GA: r = 0.408; p = 0.002). No genotype-related pattern of significance was shown for the association between dietary folate and homocysteine. When examined by gender, some differences were apparent; one-way ANOVA showed that genotype influenced diastolic blood pressure in males (p = 0.019), while only females showed a significant correlation between dietary folate and blood pressure within specific genotypes (Systolic pressure GA: r = - 0.372; p = 0.025, carriage of A: r = 0.-0.357; p = 0.011. Diastolic pressure GA: r = - 0.355; p = 0.034, carriage of A: r = 0.-0.310; p = 0.029). The G80A RFC SNP had an impact on the absorption and cellular translocation of dietary folate and its association with blood pressure in an elderly population.

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KW - Folate

KW - Homocysteine

KW - Hypertension

KW - Polymorphism

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