Gel-free species identification using melt-curve analysis

Oliver Berry, Stephen Sarre

    Research output: Contribution to journal › Article

    49 Citations (Scopus)

    Abstract

    DNA-based identification of organisms is an important tool in biosecurity, ecological monitoring and wildlife forensics. Current methods usually involve post-polymerase chain reaction (PCR) manipulations (e.g. restriction digest, gel electrophoresis), which add to the expense and time required for processing samples, and may introduce error. We developed a method of species identification that uses species-specific primers and melt-curve analysis, and avoids post-PCR manipulation of samples. The method was highly accurate when trialled on DNA from six large carnivore species from Tasmania, Australia. Because of its flexibility and cost-effectiveness, this method should find wide use in many areas of applied biological science
    Original languageEnglish
    Pages (from-to)1-4
    Number of pages4
    JournalMolecular Ecology Resources
    Volume7
    Issue number1
    DOIs
    Publication statusPublished - 2007

    Fingerprint

    gel
    Gels
    gels
    melt
    polymerase chain reaction
    Tasmania
    DNA
    Polymerase Chain Reaction
    Biological Science Disciplines
    biosecurity
    cost effectiveness
    carnivore
    methodology
    carnivores
    gel electrophoresis
    Cost-Benefit Analysis
    Electrophoresis
    wildlife
    electrokinesis
    sampling

    Cite this

    Berry, Oliver ; Sarre, Stephen. / Gel-free species identification using melt-curve analysis. In: Molecular Ecology Resources. 2007 ; Vol. 7, No. 1. pp. 1-4.
    @article{0fe2b585ac184ef6b7cfea1cfa58eb6d,
    title = "Gel-free species identification using melt-curve analysis",
    abstract = "DNA-based identification of organisms is an important tool in biosecurity, ecological monitoring and wildlife forensics. Current methods usually involve post-polymerase chain reaction (PCR) manipulations (e.g. restriction digest, gel electrophoresis), which add to the expense and time required for processing samples, and may introduce error. We developed a method of species identification that uses species-specific primers and melt-curve analysis, and avoids post-PCR manipulation of samples. The method was highly accurate when trialled on DNA from six large carnivore species from Tasmania, Australia. Because of its flexibility and cost-effectiveness, this method should find wide use in many areas of applied biological science",
    author = "Oliver Berry and Stephen Sarre",
    year = "2007",
    doi = "10.1111/j.1471-8286.2006.01541.x",
    language = "English",
    volume = "7",
    pages = "1--4",
    journal = "Molecular Ecology Notes",
    issn = "1755-098X",
    publisher = "Wiley-Blackwell",
    number = "1",

    }

    Berry, O & Sarre, S 2007, 'Gel-free species identification using melt-curve analysis', Molecular Ecology Resources, vol. 7, no. 1, pp. 1-4. https://doi.org/10.1111/j.1471-8286.2006.01541.x

    Gel-free species identification using melt-curve analysis. / Berry, Oliver; Sarre, Stephen.

    In: Molecular Ecology Resources, Vol. 7, No. 1, 2007, p. 1-4.

    Research output: Contribution to journal › Article

    TY - JOUR

    T1 - Gel-free species identification using melt-curve analysis

    AU - Berry, Oliver

    AU - Sarre, Stephen

    PY - 2007

    Y1 - 2007

    N2 - DNA-based identification of organisms is an important tool in biosecurity, ecological monitoring and wildlife forensics. Current methods usually involve post-polymerase chain reaction (PCR) manipulations (e.g. restriction digest, gel electrophoresis), which add to the expense and time required for processing samples, and may introduce error. We developed a method of species identification that uses species-specific primers and melt-curve analysis, and avoids post-PCR manipulation of samples. The method was highly accurate when trialled on DNA from six large carnivore species from Tasmania, Australia. Because of its flexibility and cost-effectiveness, this method should find wide use in many areas of applied biological science

    AB - DNA-based identification of organisms is an important tool in biosecurity, ecological monitoring and wildlife forensics. Current methods usually involve post-polymerase chain reaction (PCR) manipulations (e.g. restriction digest, gel electrophoresis), which add to the expense and time required for processing samples, and may introduce error. We developed a method of species identification that uses species-specific primers and melt-curve analysis, and avoids post-PCR manipulation of samples. The method was highly accurate when trialled on DNA from six large carnivore species from Tasmania, Australia. Because of its flexibility and cost-effectiveness, this method should find wide use in many areas of applied biological science

    U2 - 10.1111/j.1471-8286.2006.01541.x

    DO - 10.1111/j.1471-8286.2006.01541.x

    M3 - Article

    VL - 7

    SP - 1

    EP - 4

    JO - Molecular Ecology Notes

    JF - Molecular Ecology Notes

    SN - 1755-098X

    IS - 1

    ER -

    Berry O, Sarre S. Gel-free species identification using melt-curve analysis. Molecular Ecology Resources. 2007;7(1):1-4. https://doi.org/10.1111/j.1471-8286.2006.01541.x

    Access to Document