Genetic mapping of resistance to Diuraphis noxia (Kurdjumov) biotype 2 in wheat (Triticum aestivum L.) accession CI2401

Mehdi Fazel-Najafabadi, Junhua Peng, Frank Peairs, Hana Simkova, Andrzej Kilian, Nora Lapitan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The RWA, Diuraphis noxia (Kurdjumov), is a devastating insect pest of wheat (Triticumaestivum L.) and barley (Hordeumvulgare) in the United States and in many parts of the world. The use of D. noxia-resistant cultivars is an economically useful approach for protecting cereals from this aphid. However, there are few genes conferring resistance to the most predominant US biotype (Biotype RWA2). Wheat line CI2401, originating from Tajikistan, has been identified to be resistant to RWA2. An F2-derived F3 (F2:3) segregating population developed from a cross between CI2401 and Glupro (a high quality susceptible wheat cultivar) was used to genetically map the resistance in CI2401. Seedlings from F2 individuals and F3 families were infested with RWA2 aphids. Seedling reactions were scored as resistant or susceptible based on the degrees of leaf rolling and chlorosis. The observed segregation ratios in the F2 and F3 generations indicate the presence of a major dominant gene controlling resistance to RWA2. The gene, named Dn2401, was genetically mapped to the short arm of chromosome 7D. Xbarc214 mapped 1.1 cM and Xgwm473 mapped 1.8 cM distal and proximal, respectively, to the gene. Association studies using more than 12,000 SNPs and SilicoDArTs confirmed the presence of a major signal associated with resistance on chromosome 7DS. In addition, a minor signal was detected in chromosome 1D. The markers developed in this study will be useful for marker-assisted-breeding for resistance to RWA2.
Original languageEnglish
Pages (from-to)607-614
Number of pages8
JournalEuphytica
Volume203
Issue number3
DOIs
Publication statusPublished - Jul 2015
Externally publishedYes

Fingerprint

Diuraphis noxia
biotypes
Triticum
chromosome mapping
Aphids
Triticum aestivum
Chromosomes
chromosomes
Seedlings
wheat
Aphidoidea
Tajikistan
Genes
Hypochromic Anemia
leaf rolling
Dominant Genes
genes
seedlings
dominant genes
cultivars

Cite this

Fazel-Najafabadi, Mehdi ; Peng, Junhua ; Peairs, Frank ; Simkova, Hana ; Kilian, Andrzej ; Lapitan, Nora. / Genetic mapping of resistance to Diuraphis noxia (Kurdjumov) biotype 2 in wheat (Triticum aestivum L.) accession CI2401. In: Euphytica. 2015 ; Vol. 203, No. 3. pp. 607-614.
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abstract = "The RWA, Diuraphis noxia (Kurdjumov), is a devastating insect pest of wheat (Triticumaestivum L.) and barley (Hordeumvulgare) in the United States and in many parts of the world. The use of D. noxia-resistant cultivars is an economically useful approach for protecting cereals from this aphid. However, there are few genes conferring resistance to the most predominant US biotype (Biotype RWA2). Wheat line CI2401, originating from Tajikistan, has been identified to be resistant to RWA2. An F2-derived F3 (F2:3) segregating population developed from a cross between CI2401 and Glupro (a high quality susceptible wheat cultivar) was used to genetically map the resistance in CI2401. Seedlings from F2 individuals and F3 families were infested with RWA2 aphids. Seedling reactions were scored as resistant or susceptible based on the degrees of leaf rolling and chlorosis. The observed segregation ratios in the F2 and F3 generations indicate the presence of a major dominant gene controlling resistance to RWA2. The gene, named Dn2401, was genetically mapped to the short arm of chromosome 7D. Xbarc214 mapped 1.1 cM and Xgwm473 mapped 1.8 cM distal and proximal, respectively, to the gene. Association studies using more than 12,000 SNPs and SilicoDArTs confirmed the presence of a major signal associated with resistance on chromosome 7DS. In addition, a minor signal was detected in chromosome 1D. The markers developed in this study will be useful for marker-assisted-breeding for resistance to RWA2.",
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Fazel-Najafabadi, M, Peng, J, Peairs, F, Simkova, H, Kilian, A & Lapitan, N 2015, 'Genetic mapping of resistance to Diuraphis noxia (Kurdjumov) biotype 2 in wheat (Triticum aestivum L.) accession CI2401', Euphytica, vol. 203, no. 3, pp. 607-614. https://doi.org/10.1007/s10681-014-1284-0

Genetic mapping of resistance to Diuraphis noxia (Kurdjumov) biotype 2 in wheat (Triticum aestivum L.) accession CI2401. / Fazel-Najafabadi, Mehdi; Peng, Junhua; Peairs, Frank; Simkova, Hana; Kilian, Andrzej; Lapitan, Nora.

In: Euphytica, Vol. 203, No. 3, 07.2015, p. 607-614.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Genetic mapping of resistance to Diuraphis noxia (Kurdjumov) biotype 2 in wheat (Triticum aestivum L.) accession CI2401

AU - Fazel-Najafabadi, Mehdi

AU - Peng, Junhua

AU - Peairs, Frank

AU - Simkova, Hana

AU - Kilian, Andrzej

AU - Lapitan, Nora

PY - 2015/7

Y1 - 2015/7

N2 - The RWA, Diuraphis noxia (Kurdjumov), is a devastating insect pest of wheat (Triticumaestivum L.) and barley (Hordeumvulgare) in the United States and in many parts of the world. The use of D. noxia-resistant cultivars is an economically useful approach for protecting cereals from this aphid. However, there are few genes conferring resistance to the most predominant US biotype (Biotype RWA2). Wheat line CI2401, originating from Tajikistan, has been identified to be resistant to RWA2. An F2-derived F3 (F2:3) segregating population developed from a cross between CI2401 and Glupro (a high quality susceptible wheat cultivar) was used to genetically map the resistance in CI2401. Seedlings from F2 individuals and F3 families were infested with RWA2 aphids. Seedling reactions were scored as resistant or susceptible based on the degrees of leaf rolling and chlorosis. The observed segregation ratios in the F2 and F3 generations indicate the presence of a major dominant gene controlling resistance to RWA2. The gene, named Dn2401, was genetically mapped to the short arm of chromosome 7D. Xbarc214 mapped 1.1 cM and Xgwm473 mapped 1.8 cM distal and proximal, respectively, to the gene. Association studies using more than 12,000 SNPs and SilicoDArTs confirmed the presence of a major signal associated with resistance on chromosome 7DS. In addition, a minor signal was detected in chromosome 1D. The markers developed in this study will be useful for marker-assisted-breeding for resistance to RWA2.

AB - The RWA, Diuraphis noxia (Kurdjumov), is a devastating insect pest of wheat (Triticumaestivum L.) and barley (Hordeumvulgare) in the United States and in many parts of the world. The use of D. noxia-resistant cultivars is an economically useful approach for protecting cereals from this aphid. However, there are few genes conferring resistance to the most predominant US biotype (Biotype RWA2). Wheat line CI2401, originating from Tajikistan, has been identified to be resistant to RWA2. An F2-derived F3 (F2:3) segregating population developed from a cross between CI2401 and Glupro (a high quality susceptible wheat cultivar) was used to genetically map the resistance in CI2401. Seedlings from F2 individuals and F3 families were infested with RWA2 aphids. Seedling reactions were scored as resistant or susceptible based on the degrees of leaf rolling and chlorosis. The observed segregation ratios in the F2 and F3 generations indicate the presence of a major dominant gene controlling resistance to RWA2. The gene, named Dn2401, was genetically mapped to the short arm of chromosome 7D. Xbarc214 mapped 1.1 cM and Xgwm473 mapped 1.8 cM distal and proximal, respectively, to the gene. Association studies using more than 12,000 SNPs and SilicoDArTs confirmed the presence of a major signal associated with resistance on chromosome 7DS. In addition, a minor signal was detected in chromosome 1D. The markers developed in this study will be useful for marker-assisted-breeding for resistance to RWA2.

KW - Hordeumvulgare

KW - Marker-assisted selection

KW - RWA biotype 2

KW - RWA resistance

KW - Simple sequence repeat markers

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U2 - 10.1007/s10681-014-1284-0

DO - 10.1007/s10681-014-1284-0

M3 - Article

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