TY - JOUR
T1 - Genomic Restructuring in the Tasmanian Devil Facial Tumour
T2 - Chromosome Painting and Gene Mapping Provide Clues to Evolution of a Transmissible Tumour
AU - Deakin, Janine
AU - Bender, Hannah
AU - Pearse, Anne-Maree
AU - Rens, Willem
AU - O'Brien, Patricia
AU - Ferguson-Smith, Malcolm
AU - Cheng, Yuanyuan
AU - Morris, Katrina
AU - Taylor, Robyn
AU - Stuart, Andrew
AU - Belov, Katherine
AU - Amemiya, Chris
AU - Murchison, Elizabeth
AU - Papenfuss, Anthony
AU - Marshall Graves, Jennifer
PY - 2012
Y1 - 2012
N2 - Devil facial tumour disease (DFTD) is a fatal, transmissible malignancy that threatens the world’s largest marsupial carnivore, the Tasmanian devil, with extinction. First recognised in 1996, DFTD has had a catastrophic effect on wild devil numbers, and intense research efforts to understand and contain the disease have since demonstrated that the tumour is a clonal cell line transmitted by allograft. We used chromosome painting and gene mapping to deconstruct the DFTD karyotype and determine the chromosome and gene rearrangements involved in carcinogenesis. Chromosome painting on three different DFTD tumour strains determined the origins of marker chromosomes and provided a general overview of the rearrangement in DFTD karyotypes. Mapping of 105 BAC clones by fluorescence in situ hybridisation provided a finer level of resolution of genome rearrangements in DFTD strains. Our findings demonstrate that only limited regions of the genome, mainly chromosomes 1 and X, are rearranged in DFTD. Regions rearranged in DFTD are also highly rearranged between different marsupials. Differences between strains are limited, reflecting the unusually stable nature of DFTD. Finally, our detailed maps of both the devil and tumour karyotypes provide a physical framework for future genomic investigations into DFTD.
AB - Devil facial tumour disease (DFTD) is a fatal, transmissible malignancy that threatens the world’s largest marsupial carnivore, the Tasmanian devil, with extinction. First recognised in 1996, DFTD has had a catastrophic effect on wild devil numbers, and intense research efforts to understand and contain the disease have since demonstrated that the tumour is a clonal cell line transmitted by allograft. We used chromosome painting and gene mapping to deconstruct the DFTD karyotype and determine the chromosome and gene rearrangements involved in carcinogenesis. Chromosome painting on three different DFTD tumour strains determined the origins of marker chromosomes and provided a general overview of the rearrangement in DFTD karyotypes. Mapping of 105 BAC clones by fluorescence in situ hybridisation provided a finer level of resolution of genome rearrangements in DFTD strains. Our findings demonstrate that only limited regions of the genome, mainly chromosomes 1 and X, are rearranged in DFTD. Regions rearranged in DFTD are also highly rearranged between different marsupials. Differences between strains are limited, reflecting the unusually stable nature of DFTD. Finally, our detailed maps of both the devil and tumour karyotypes provide a physical framework for future genomic investigations into DFTD.
U2 - 10.1371/journal.pgen.1002483
DO - 10.1371/journal.pgen.1002483
M3 - Article
SN - 1553-7390
VL - 8
SP - 1
EP - 16
JO - PLoS Genetics
JF - PLoS Genetics
ER -