Hepatitis C virus replication in cell culture

Michael Frese; Volker Lohmann; Thomas Pietschmann; Artur Kaul; Nicole Krieger; Jens Bukh; Ralf Bartenschlager

doi:10.1007/978-4-431-53977-3_11

Hepatitis C virus replication in cell culture

Michael Frese, Volker Lohmann, Thomas Pietschmann, Artur Kaul, Nicole Krieger, Jens Bukh, Ralf Bartenschlager

Research output: A Conference proceeding or a Chapter in Book › Conference contribution › peer-review

Abstract

By using self-replicating hepatitis C virus (HCV) RNAs, it is now possible to investigate various aspects of the viral replication cycle in cell culture. Here, we describe the construction of subgenomic and genomic replicons which replicate to high levels in the human hepatoma cell line Huh-7. Furthermore, we report and discuss recent contributions from our laboratory which has provided a better understanding of (i) HCV particle formation, (ii) the mechanisms behind the cell culture-adaptation of HCV, and (iii) the innate immune response against HCV

Original language	English
Title of host publication	Therapy for Viral Hepatitis and Prevention of Hepatocellular Carcinoma
Editors	M Omata, K Okita
Place of Publication	Tokyo
Publisher	Springer
Pages	108-122
Number of pages	15
ISBN (Electronic)	9784431539773
ISBN (Print)	9784431679752
DOIs	https://doi.org/10.1007/978-4-431-53977-3_11
Publication status	Published - 2004
Event	First JSH Single Topic Conference - , Japan Duration: 14 Nov 2002 → 15 Nov 2002

Conference

Conference	First JSH Single Topic Conference
Country/Territory	Japan
Period	14/11/02 → 15/11/02

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-4-431-53977-3_11

Cite this

@inproceedings{410b1de55950473895279010bf69fc6c,

title = "Hepatitis C virus replication in cell culture",

abstract = "By using self-replicating hepatitis C virus (HCV) RNAs, it is now possible to investigate various aspects of the viral replication cycle in cell culture. Here, we describe the construction of subgenomic and genomic replicons which replicate to high levels in the human hepatoma cell line Huh-7. Furthermore, we report and discuss recent contributions from our laboratory which has provided a better understanding of (i) HCV particle formation, (ii) the mechanisms behind the cell culture-adaptation of HCV, and (iii) the innate immune response against HCV",

author = "Michael Frese and Volker Lohmann and Thomas Pietschmann and Artur Kaul and Nicole Krieger and Jens Bukh and Ralf Bartenschlager",

year = "2004",

doi = "10.1007/978-4-431-53977-3_11",

language = "English",

isbn = "9784431679752",

pages = "108--122",

editor = "M Omata and K Okita",

booktitle = "Therapy for Viral Hepatitis and Prevention of Hepatocellular Carcinoma",

publisher = "Springer",

address = "Netherlands",

note = "First JSH Single Topic Conference ; Conference date: 14-11-2002 Through 15-11-2002",

}

TY - GEN

T1 - Hepatitis C virus replication in cell culture

AU - Frese, Michael

AU - Lohmann, Volker

AU - Pietschmann, Thomas

AU - Kaul, Artur

AU - Krieger, Nicole

AU - Bukh, Jens

AU - Bartenschlager, Ralf

PY - 2004

Y1 - 2004

N2 - By using self-replicating hepatitis C virus (HCV) RNAs, it is now possible to investigate various aspects of the viral replication cycle in cell culture. Here, we describe the construction of subgenomic and genomic replicons which replicate to high levels in the human hepatoma cell line Huh-7. Furthermore, we report and discuss recent contributions from our laboratory which has provided a better understanding of (i) HCV particle formation, (ii) the mechanisms behind the cell culture-adaptation of HCV, and (iii) the innate immune response against HCV

AB - By using self-replicating hepatitis C virus (HCV) RNAs, it is now possible to investigate various aspects of the viral replication cycle in cell culture. Here, we describe the construction of subgenomic and genomic replicons which replicate to high levels in the human hepatoma cell line Huh-7. Furthermore, we report and discuss recent contributions from our laboratory which has provided a better understanding of (i) HCV particle formation, (ii) the mechanisms behind the cell culture-adaptation of HCV, and (iii) the innate immune response against HCV

U2 - 10.1007/978-4-431-53977-3_11

DO - 10.1007/978-4-431-53977-3_11

M3 - Conference contribution

SN - 9784431679752

SP - 108

EP - 122

BT - Therapy for Viral Hepatitis and Prevention of Hepatocellular Carcinoma

A2 - Omata, M

A2 - Okita, K

PB - Springer

CY - Tokyo

T2 - First JSH Single Topic Conference

Y2 - 14 November 2002 through 15 November 2002

ER -

Hepatitis C virus replication in cell culture

Abstract

Conference

UN SDGs

Access to Document

Fingerprint

Cite this