TY - JOUR
T1 - Hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability
AU - Martínez-López, Lina María
AU - Perez-Gonzalez, Alexis
AU - Washington, Elizabeth Ann
AU - Woodward, Andrew P.
AU - Roth-Schulze, Alexandra Jazmin
AU - Dandrieux, Julien R.S.
AU - Johnstone, Thurid
AU - Prakash, Nathalee
AU - Jex, Aaron
AU - Mansfield, Caroline
N1 - Funding Information:
This work was supported by the International and Postgraduate Research Scholarship (IPRS) from The Australian Government 2014 (LM). This work was funded by The Comparative and Gastroenterology Society/ Waltham Grant 2016 (LM) [https://vetmed.tamu. edu/cgs/about/] and Early Career Researcher funding through the University of Melbourne 2018 (CM). The sponsors played no role in study design, data collection/analysis and were not involved in preparation of the publication or decision to publish. The authors acknowledge the support of Stephen Wilcox and Katharina Stracke in the 16S rRNA gene sequencing, Andrew Stent for the histopathology analysis and Leilani Santos for her support during the experiments and all the staff and nurses from the U-Vet hospital at the Faculty of Veterinary and Agricultural Sciences at the University of Melbourne for their help in the collection of samples.
Publisher Copyright:
© 2021 Martínez-López et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2021/8
Y1 - 2021/8
N2 - Chronic enteropathies are a common problem in dogs, but many aspects of the pathogenesis remain unknown, making the therapeutic approach challenging in some cases. Environmental factors are intimately related to the development and perpetuation of gastrointestinal disease and the gut microbiome has been identified as a contributing factor. Previous studies have identified dysbiosis and reduced bacterial diversity in the gastrointestinal microbiota of dogs with chronic enteropathies. In this case-controlled study, we use flow cytometry and 16S rRNA sequencing to characterise bacteria highly coated with IgA or IgG in faecal samples from dogs with chronic enteropathy and evaluated their correlation with disease and resolution of the clinical signs. IgA and IgG-coated faecal bacterial counts were significantly higher during active disease compared to healthy dogs and decreased with the resolution of the clinical signs. Characterisation of taxa-specific coating of the intestinal microbiota with IgA and IgG showed marked variation between dogs and disease states, and different patterns of immunoglobulin enrichment were observed in dogs with chronic enteropathy, particularly for Erysipelotrichaceae, Clostridicaceae, Enterobacteriaceae, Prevotellaceae and Bacteroidaceae, families. Although, members of these bacterial groups have been associated with strong immunogenic properties and could potentially constitute important biomarkers of disease, their significance and role need to be further investigated.
AB - Chronic enteropathies are a common problem in dogs, but many aspects of the pathogenesis remain unknown, making the therapeutic approach challenging in some cases. Environmental factors are intimately related to the development and perpetuation of gastrointestinal disease and the gut microbiome has been identified as a contributing factor. Previous studies have identified dysbiosis and reduced bacterial diversity in the gastrointestinal microbiota of dogs with chronic enteropathies. In this case-controlled study, we use flow cytometry and 16S rRNA sequencing to characterise bacteria highly coated with IgA or IgG in faecal samples from dogs with chronic enteropathy and evaluated their correlation with disease and resolution of the clinical signs. IgA and IgG-coated faecal bacterial counts were significantly higher during active disease compared to healthy dogs and decreased with the resolution of the clinical signs. Characterisation of taxa-specific coating of the intestinal microbiota with IgA and IgG showed marked variation between dogs and disease states, and different patterns of immunoglobulin enrichment were observed in dogs with chronic enteropathy, particularly for Erysipelotrichaceae, Clostridicaceae, Enterobacteriaceae, Prevotellaceae and Bacteroidaceae, families. Although, members of these bacterial groups have been associated with strong immunogenic properties and could potentially constitute important biomarkers of disease, their significance and role need to be further investigated.
UR - http://www.scopus.com/inward/record.url?scp=85113286086&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0255012
DO - 10.1371/journal.pone.0255012
M3 - Article
C2 - 34411114
AN - SCOPUS:85113286086
SN - 1932-6203
VL - 16
SP - 1
EP - 22
JO - PLoS One
JF - PLoS One
IS - 8 August
M1 - e0255012
ER -