High Human Bocavirus Viral Load Is Associated with Disease Severity in Children under Five Years of Age

Baihui Zhao, Xuelian Yu, Chuanxian Wang, Zheng Teng, Chun Wang, Jiaren Shen, Ye Gao, Zhaokui Zhu, Jiayu Wang, Zhengan Yuan, Fan Wu, Xi Zhang, Reena GHILDYAL

Research output: Contribution to journalArticle

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Abstract

Human bocavirus (HBoV) is a parvovirus and detected worldwide in lower respiratory tract infections (LRTIs), but its pathogenic role in respiratory illness is still debatable due to high incidence of co-infection with other respiratory viruses. To determine the prevalence of HBoV infection in patients with LRTI in Shanghai and its correlation with disease severity, we performed a 3-year prospective study of HBoV in healthy controls, outpatients and inpatients under five years of age with X-ray diagnosed LRTIs. Nasopharyngeal aspirates were tested by PCR for common respiratory viruses and by real time PCR for HBoV subtypes 1–4. Nasopharyngeal swabs from healthy controls and serum samples and stools from inpatients were also tested for HBoV1-4 by real time PCR. Viral loads were determined by quantitative real time PCR in all HBoV positive samples. HBoV1 was detected in 7.0% of inpatients, with annual rates of 5.1%, 8.0% and 4.8% in 2010, 2011 and 2012, respectively. Respiratory syncytial virus (RSV) subtype A was the most frequent co-infection detected; HBoV1 and RSVA appeared to co-circulate with similar seasonal variations. High HBoV viral loads (>106 copies/ml) were significantly more frequent in inpatients and outpatients than in healthy controls. There was a direct correlation of high viral load with increasing disease severity in patients co-infected with HBoV1 and at least one other respiratory virus. In summary, our data suggest that HBoV1 can cause LRTIs, but symptomatic HBoV infection is only observed in the context of high viral load
Original languageEnglish
Pages (from-to)e62318-e62318
Number of pages1
JournalPLoS One
Volume8
Issue number4
DOIs
Publication statusPublished - 30 Apr 2013

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Bocavirus
Human bocavirus
viral load
Viral Load
Viruses
disease severity
Respiratory Tract Infections
Inpatients
respiratory tract diseases
Real-Time Polymerase Chain Reaction
viruses
quantitative polymerase chain reaction
Coinfection
mixed infection
Outpatients
Parvovirus
Respiratory Syncytial Viruses
X rays
Protoparvovirus
Infection

Cite this

Zhao, Baihui ; Yu, Xuelian ; Wang, Chuanxian ; Teng, Zheng ; Wang, Chun ; Shen, Jiaren ; Gao, Ye ; Zhu, Zhaokui ; Wang, Jiayu ; Yuan, Zhengan ; Wu, Fan ; Zhang, Xi ; GHILDYAL, Reena. / High Human Bocavirus Viral Load Is Associated with Disease Severity in Children under Five Years of Age. In: PLoS One. 2013 ; Vol. 8, No. 4. pp. e62318-e62318.
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abstract = "Human bocavirus (HBoV) is a parvovirus and detected worldwide in lower respiratory tract infections (LRTIs), but its pathogenic role in respiratory illness is still debatable due to high incidence of co-infection with other respiratory viruses. To determine the prevalence of HBoV infection in patients with LRTI in Shanghai and its correlation with disease severity, we performed a 3-year prospective study of HBoV in healthy controls, outpatients and inpatients under five years of age with X-ray diagnosed LRTIs. Nasopharyngeal aspirates were tested by PCR for common respiratory viruses and by real time PCR for HBoV subtypes 1–4. Nasopharyngeal swabs from healthy controls and serum samples and stools from inpatients were also tested for HBoV1-4 by real time PCR. Viral loads were determined by quantitative real time PCR in all HBoV positive samples. HBoV1 was detected in 7.0{\%} of inpatients, with annual rates of 5.1{\%}, 8.0{\%} and 4.8{\%} in 2010, 2011 and 2012, respectively. Respiratory syncytial virus (RSV) subtype A was the most frequent co-infection detected; HBoV1 and RSVA appeared to co-circulate with similar seasonal variations. High HBoV viral loads (>106 copies/ml) were significantly more frequent in inpatients and outpatients than in healthy controls. There was a direct correlation of high viral load with increasing disease severity in patients co-infected with HBoV1 and at least one other respiratory virus. In summary, our data suggest that HBoV1 can cause LRTIs, but symptomatic HBoV infection is only observed in the context of high viral load",
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author = "Baihui Zhao and Xuelian Yu and Chuanxian Wang and Zheng Teng and Chun Wang and Jiaren Shen and Ye Gao and Zhaokui Zhu and Jiayu Wang and Zhengan Yuan and Fan Wu and Xi Zhang and Reena GHILDYAL",
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Zhao, B, Yu, X, Wang, C, Teng, Z, Wang, C, Shen, J, Gao, Y, Zhu, Z, Wang, J, Yuan, Z, Wu, F, Zhang, X & GHILDYAL, R 2013, 'High Human Bocavirus Viral Load Is Associated with Disease Severity in Children under Five Years of Age', PLoS One, vol. 8, no. 4, pp. e62318-e62318. https://doi.org/10.1371/journal.pone.0062318

High Human Bocavirus Viral Load Is Associated with Disease Severity in Children under Five Years of Age. / Zhao, Baihui; Yu, Xuelian; Wang, Chuanxian; Teng, Zheng; Wang, Chun; Shen, Jiaren; Gao, Ye; Zhu, Zhaokui; Wang, Jiayu; Yuan, Zhengan; Wu, Fan; Zhang, Xi; GHILDYAL, Reena.

In: PLoS One, Vol. 8, No. 4, 30.04.2013, p. e62318-e62318.

Research output: Contribution to journalArticle

TY - JOUR

T1 - High Human Bocavirus Viral Load Is Associated with Disease Severity in Children under Five Years of Age

AU - Zhao, Baihui

AU - Yu, Xuelian

AU - Wang, Chuanxian

AU - Teng, Zheng

AU - Wang, Chun

AU - Shen, Jiaren

AU - Gao, Ye

AU - Zhu, Zhaokui

AU - Wang, Jiayu

AU - Yuan, Zhengan

AU - Wu, Fan

AU - Zhang, Xi

AU - GHILDYAL, Reena

PY - 2013/4/30

Y1 - 2013/4/30

N2 - Human bocavirus (HBoV) is a parvovirus and detected worldwide in lower respiratory tract infections (LRTIs), but its pathogenic role in respiratory illness is still debatable due to high incidence of co-infection with other respiratory viruses. To determine the prevalence of HBoV infection in patients with LRTI in Shanghai and its correlation with disease severity, we performed a 3-year prospective study of HBoV in healthy controls, outpatients and inpatients under five years of age with X-ray diagnosed LRTIs. Nasopharyngeal aspirates were tested by PCR for common respiratory viruses and by real time PCR for HBoV subtypes 1–4. Nasopharyngeal swabs from healthy controls and serum samples and stools from inpatients were also tested for HBoV1-4 by real time PCR. Viral loads were determined by quantitative real time PCR in all HBoV positive samples. HBoV1 was detected in 7.0% of inpatients, with annual rates of 5.1%, 8.0% and 4.8% in 2010, 2011 and 2012, respectively. Respiratory syncytial virus (RSV) subtype A was the most frequent co-infection detected; HBoV1 and RSVA appeared to co-circulate with similar seasonal variations. High HBoV viral loads (>106 copies/ml) were significantly more frequent in inpatients and outpatients than in healthy controls. There was a direct correlation of high viral load with increasing disease severity in patients co-infected with HBoV1 and at least one other respiratory virus. In summary, our data suggest that HBoV1 can cause LRTIs, but symptomatic HBoV infection is only observed in the context of high viral load

AB - Human bocavirus (HBoV) is a parvovirus and detected worldwide in lower respiratory tract infections (LRTIs), but its pathogenic role in respiratory illness is still debatable due to high incidence of co-infection with other respiratory viruses. To determine the prevalence of HBoV infection in patients with LRTI in Shanghai and its correlation with disease severity, we performed a 3-year prospective study of HBoV in healthy controls, outpatients and inpatients under five years of age with X-ray diagnosed LRTIs. Nasopharyngeal aspirates were tested by PCR for common respiratory viruses and by real time PCR for HBoV subtypes 1–4. Nasopharyngeal swabs from healthy controls and serum samples and stools from inpatients were also tested for HBoV1-4 by real time PCR. Viral loads were determined by quantitative real time PCR in all HBoV positive samples. HBoV1 was detected in 7.0% of inpatients, with annual rates of 5.1%, 8.0% and 4.8% in 2010, 2011 and 2012, respectively. Respiratory syncytial virus (RSV) subtype A was the most frequent co-infection detected; HBoV1 and RSVA appeared to co-circulate with similar seasonal variations. High HBoV viral loads (>106 copies/ml) were significantly more frequent in inpatients and outpatients than in healthy controls. There was a direct correlation of high viral load with increasing disease severity in patients co-infected with HBoV1 and at least one other respiratory virus. In summary, our data suggest that HBoV1 can cause LRTIs, but symptomatic HBoV infection is only observed in the context of high viral load

KW - Rhinovirus

KW - Nuclear Transport

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