High-polyphenol chocolate reduces endothelial dysfunction and oxidative stress during acute transient hyperglycaemia in Type 2 diabetes: a pilot randomized controlled trial

Duane MELLOR, Leigh Madden, Karen Smith, Eric Kilpatrick, Stephen Atkin

Research output: Contribution to journalArticle

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Abstract

Aims To investigate the effects of high-polyphenol chocolate upon endothelial function and oxidative stress in Type2 diabetes mellitus during acute transient hyperglycaemia induced following a 75-g oral glucose challenge. Methods Ten subjects with Type2 diabetes underwent a double-blinded randomized controlled crossover study. A 75-g oral glucose load was used to induce hyperglycaemia, which was administered to participants 60min after they had ingested either low (control) or high-polyphenol chocolate. Participants undertook testing at weekly intervals, following an initial cocoa-free period. Endothelial function was assessed by both functional [reactive hyperaemia peripheral artery tonometry (EndoPAT-2000) and serum markers (including intercellular adhesion molecule1, P-selectin and P-selectin glycoprotein ligand1]. Urinary 15-F2t-isoprostane adjusted for creatinine was used as an oxidative stress marker. Measurements were made at baseline and 2h post-ingestion of the glucose load. Results Prior consumption of high-polyphenol chocolate before a glucose load improved endothelial function (1.7 +/- 0.1 vs. 2.3 +/- 0.1%, P=0.01), whereas prior consumption of control chocolate resulted in a significant increase in intercellular adhesion molecule1 (321.1 +/- 7.6 vs. 373.6 +/- 10.5ng/ml, P=0.04) and 15-F2t-isoprostane (116.8 +/- 5.7 vs. 207.1 +/- 5.7mg/mol, P=0.02). Analysis of percentage changes from baseline comparing control and high-polyphenol chocolate showed a significant improvement for high-polyphenol chocolate in both measures of endothelial function (P<0.05) and for urinary 15-F2t-isoprostane (P=0.04). Conclusion High-polyphenol chocolate protected against acute hyperglycaemia-induced endothelial dysfunction and oxidative stress in individuals with Type2 diabetes mellitus
Original languageEnglish
Pages (from-to)478-483
Number of pages6
JournalDiabetic Medicine
Volume30
Issue number4
DOIs
Publication statusPublished - 2013
Externally publishedYes

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Polyphenols
Hyperglycemia
Type 2 Diabetes Mellitus
Oxidative Stress
Randomized Controlled Trials
Glucose
Diabetes Mellitus
P-Selectin
Hyperemia
Manometry
P-Glycoprotein
Chocolate
Cross-Over Studies
Creatinine
Arteries
Eating
Biomarkers
15-F2t-isoprostane

Cite this

MELLOR, Duane ; Madden, Leigh ; Smith, Karen ; Kilpatrick, Eric ; Atkin, Stephen. / High-polyphenol chocolate reduces endothelial dysfunction and oxidative stress during acute transient hyperglycaemia in Type 2 diabetes: a pilot randomized controlled trial. In: Diabetic Medicine. 2013 ; Vol. 30, No. 4. pp. 478-483.
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title = "High-polyphenol chocolate reduces endothelial dysfunction and oxidative stress during acute transient hyperglycaemia in Type 2 diabetes: a pilot randomized controlled trial",
abstract = "Aims To investigate the effects of high-polyphenol chocolate upon endothelial function and oxidative stress in Type2 diabetes mellitus during acute transient hyperglycaemia induced following a 75-g oral glucose challenge. Methods Ten subjects with Type2 diabetes underwent a double-blinded randomized controlled crossover study. A 75-g oral glucose load was used to induce hyperglycaemia, which was administered to participants 60min after they had ingested either low (control) or high-polyphenol chocolate. Participants undertook testing at weekly intervals, following an initial cocoa-free period. Endothelial function was assessed by both functional [reactive hyperaemia peripheral artery tonometry (EndoPAT-2000) and serum markers (including intercellular adhesion molecule1, P-selectin and P-selectin glycoprotein ligand1]. Urinary 15-F2t-isoprostane adjusted for creatinine was used as an oxidative stress marker. Measurements were made at baseline and 2h post-ingestion of the glucose load. Results Prior consumption of high-polyphenol chocolate before a glucose load improved endothelial function (1.7 +/- 0.1 vs. 2.3 +/- 0.1{\%}, P=0.01), whereas prior consumption of control chocolate resulted in a significant increase in intercellular adhesion molecule1 (321.1 +/- 7.6 vs. 373.6 +/- 10.5ng/ml, P=0.04) and 15-F2t-isoprostane (116.8 +/- 5.7 vs. 207.1 +/- 5.7mg/mol, P=0.02). Analysis of percentage changes from baseline comparing control and high-polyphenol chocolate showed a significant improvement for high-polyphenol chocolate in both measures of endothelial function (P<0.05) and for urinary 15-F2t-isoprostane (P=0.04). Conclusion High-polyphenol chocolate protected against acute hyperglycaemia-induced endothelial dysfunction and oxidative stress in individuals with Type2 diabetes mellitus",
author = "Duane MELLOR and Leigh Madden and Karen Smith and Eric Kilpatrick and Stephen Atkin",
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doi = "10.1111/dme.12030",
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pages = "478--483",
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High-polyphenol chocolate reduces endothelial dysfunction and oxidative stress during acute transient hyperglycaemia in Type 2 diabetes: a pilot randomized controlled trial. / MELLOR, Duane; Madden, Leigh; Smith, Karen; Kilpatrick, Eric; Atkin, Stephen.

In: Diabetic Medicine, Vol. 30, No. 4, 2013, p. 478-483.

Research output: Contribution to journalArticle

TY - JOUR

T1 - High-polyphenol chocolate reduces endothelial dysfunction and oxidative stress during acute transient hyperglycaemia in Type 2 diabetes: a pilot randomized controlled trial

AU - MELLOR, Duane

AU - Madden, Leigh

AU - Smith, Karen

AU - Kilpatrick, Eric

AU - Atkin, Stephen

PY - 2013

Y1 - 2013

N2 - Aims To investigate the effects of high-polyphenol chocolate upon endothelial function and oxidative stress in Type2 diabetes mellitus during acute transient hyperglycaemia induced following a 75-g oral glucose challenge. Methods Ten subjects with Type2 diabetes underwent a double-blinded randomized controlled crossover study. A 75-g oral glucose load was used to induce hyperglycaemia, which was administered to participants 60min after they had ingested either low (control) or high-polyphenol chocolate. Participants undertook testing at weekly intervals, following an initial cocoa-free period. Endothelial function was assessed by both functional [reactive hyperaemia peripheral artery tonometry (EndoPAT-2000) and serum markers (including intercellular adhesion molecule1, P-selectin and P-selectin glycoprotein ligand1]. Urinary 15-F2t-isoprostane adjusted for creatinine was used as an oxidative stress marker. Measurements were made at baseline and 2h post-ingestion of the glucose load. Results Prior consumption of high-polyphenol chocolate before a glucose load improved endothelial function (1.7 +/- 0.1 vs. 2.3 +/- 0.1%, P=0.01), whereas prior consumption of control chocolate resulted in a significant increase in intercellular adhesion molecule1 (321.1 +/- 7.6 vs. 373.6 +/- 10.5ng/ml, P=0.04) and 15-F2t-isoprostane (116.8 +/- 5.7 vs. 207.1 +/- 5.7mg/mol, P=0.02). Analysis of percentage changes from baseline comparing control and high-polyphenol chocolate showed a significant improvement for high-polyphenol chocolate in both measures of endothelial function (P<0.05) and for urinary 15-F2t-isoprostane (P=0.04). Conclusion High-polyphenol chocolate protected against acute hyperglycaemia-induced endothelial dysfunction and oxidative stress in individuals with Type2 diabetes mellitus

AB - Aims To investigate the effects of high-polyphenol chocolate upon endothelial function and oxidative stress in Type2 diabetes mellitus during acute transient hyperglycaemia induced following a 75-g oral glucose challenge. Methods Ten subjects with Type2 diabetes underwent a double-blinded randomized controlled crossover study. A 75-g oral glucose load was used to induce hyperglycaemia, which was administered to participants 60min after they had ingested either low (control) or high-polyphenol chocolate. Participants undertook testing at weekly intervals, following an initial cocoa-free period. Endothelial function was assessed by both functional [reactive hyperaemia peripheral artery tonometry (EndoPAT-2000) and serum markers (including intercellular adhesion molecule1, P-selectin and P-selectin glycoprotein ligand1]. Urinary 15-F2t-isoprostane adjusted for creatinine was used as an oxidative stress marker. Measurements were made at baseline and 2h post-ingestion of the glucose load. Results Prior consumption of high-polyphenol chocolate before a glucose load improved endothelial function (1.7 +/- 0.1 vs. 2.3 +/- 0.1%, P=0.01), whereas prior consumption of control chocolate resulted in a significant increase in intercellular adhesion molecule1 (321.1 +/- 7.6 vs. 373.6 +/- 10.5ng/ml, P=0.04) and 15-F2t-isoprostane (116.8 +/- 5.7 vs. 207.1 +/- 5.7mg/mol, P=0.02). Analysis of percentage changes from baseline comparing control and high-polyphenol chocolate showed a significant improvement for high-polyphenol chocolate in both measures of endothelial function (P<0.05) and for urinary 15-F2t-isoprostane (P=0.04). Conclusion High-polyphenol chocolate protected against acute hyperglycaemia-induced endothelial dysfunction and oxidative stress in individuals with Type2 diabetes mellitus

U2 - 10.1111/dme.12030

DO - 10.1111/dme.12030

M3 - Article

VL - 30

SP - 478

EP - 483

JO - Diabetic Medicine

JF - Diabetic Medicine

SN - 0742-3071

IS - 4

ER -