Highly conserved Z and molecularly diverged W chromosomes in the fish genus Triportheus (Characiformes, Triportheidae)

C Yano, L Bertollo, Tariq EZAZ, V Trifonov, A Sember, T Liehr, M Cioffi

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    The main objectives of this study were to test: (1) whether the W-chromosome differentiation matches to species' evolutionary divergence (phylogenetic concordance) and (2) whether sex chromosomes share a common ancestor within a congeneric group. The monophyletic genus Triportheus (Characiformes, Triportheidae) was the model group for this study. All species in this genus so far analyzed have ZW sex chromosome system, where the Z is always the largest chromosome of the karyotype, whereas the W chromosome is highly variable ranging from almost homomorphic to highly heteromorphic. We applied conventional and molecular cytogenetic approaches including C-banding, ribosomal DNA mapping, comparative genomic hybridization (CGH) and cross-species whole chromosome painting (WCP) to test our questions. We developed Z- and W-chromosome paints from T. auritus for cross-species WCP and performed CGH in a representative species (T. signatus) to decipher level of homologies and rates of differentiation of W chromosomes. Our study revealed that the ZW sex chromosome system had a common origin, showing highly conserved Z chromosomes and remarkably divergent W chromosomes. Notably, the W chromosomes have evolved to different shapes and sequence contents within ∼15-25 Myr of divergence time. Such differentiation highlights a dynamic process of W-chromosome evolution within congeneric species of Triportheus.

    Original languageEnglish
    Pages (from-to)276-283
    Number of pages8
    JournalHeredity
    Volume118
    Issue number3
    DOIs
    Publication statusPublished - 1 Mar 2017

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    Characiformes
    Fishes
    Chromosomes
    Sex Chromosomes
    Chromosome Painting
    Comparative Genomic Hybridization
    Paint
    Ribosomal DNA
    Karyotype
    Cytogenetics

    Cite this

    Yano, C ; Bertollo, L ; EZAZ, Tariq ; Trifonov, V ; Sember, A ; Liehr, T ; Cioffi, M. / Highly conserved Z and molecularly diverged W chromosomes in the fish genus Triportheus (Characiformes, Triportheidae). In: Heredity. 2017 ; Vol. 118, No. 3. pp. 276-283.
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    Highly conserved Z and molecularly diverged W chromosomes in the fish genus Triportheus (Characiformes, Triportheidae). / Yano, C; Bertollo, L; EZAZ, Tariq; Trifonov, V; Sember, A; Liehr, T; Cioffi, M.

    In: Heredity, Vol. 118, No. 3, 01.03.2017, p. 276-283.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Highly conserved Z and molecularly diverged W chromosomes in the fish genus Triportheus (Characiformes, Triportheidae)

    AU - Yano, C

    AU - Bertollo, L

    AU - EZAZ, Tariq

    AU - Trifonov, V

    AU - Sember, A

    AU - Liehr, T

    AU - Cioffi, M

    PY - 2017/3/1

    Y1 - 2017/3/1

    N2 - The main objectives of this study were to test: (1) whether the W-chromosome differentiation matches to species' evolutionary divergence (phylogenetic concordance) and (2) whether sex chromosomes share a common ancestor within a congeneric group. The monophyletic genus Triportheus (Characiformes, Triportheidae) was the model group for this study. All species in this genus so far analyzed have ZW sex chromosome system, where the Z is always the largest chromosome of the karyotype, whereas the W chromosome is highly variable ranging from almost homomorphic to highly heteromorphic. We applied conventional and molecular cytogenetic approaches including C-banding, ribosomal DNA mapping, comparative genomic hybridization (CGH) and cross-species whole chromosome painting (WCP) to test our questions. We developed Z- and W-chromosome paints from T. auritus for cross-species WCP and performed CGH in a representative species (T. signatus) to decipher level of homologies and rates of differentiation of W chromosomes. Our study revealed that the ZW sex chromosome system had a common origin, showing highly conserved Z chromosomes and remarkably divergent W chromosomes. Notably, the W chromosomes have evolved to different shapes and sequence contents within ∼15-25 Myr of divergence time. Such differentiation highlights a dynamic process of W-chromosome evolution within congeneric species of Triportheus.

    AB - The main objectives of this study were to test: (1) whether the W-chromosome differentiation matches to species' evolutionary divergence (phylogenetic concordance) and (2) whether sex chromosomes share a common ancestor within a congeneric group. The monophyletic genus Triportheus (Characiformes, Triportheidae) was the model group for this study. All species in this genus so far analyzed have ZW sex chromosome system, where the Z is always the largest chromosome of the karyotype, whereas the W chromosome is highly variable ranging from almost homomorphic to highly heteromorphic. We applied conventional and molecular cytogenetic approaches including C-banding, ribosomal DNA mapping, comparative genomic hybridization (CGH) and cross-species whole chromosome painting (WCP) to test our questions. We developed Z- and W-chromosome paints from T. auritus for cross-species WCP and performed CGH in a representative species (T. signatus) to decipher level of homologies and rates of differentiation of W chromosomes. Our study revealed that the ZW sex chromosome system had a common origin, showing highly conserved Z chromosomes and remarkably divergent W chromosomes. Notably, the W chromosomes have evolved to different shapes and sequence contents within ∼15-25 Myr of divergence time. Such differentiation highlights a dynamic process of W-chromosome evolution within congeneric species of Triportheus.

    KW - Animals

    KW - Biological Evolution

    KW - Characidae/genetics

    KW - Chromosome Mapping

    KW - Chromosome Painting

    KW - Comparative Genomic Hybridization

    KW - DNA, Ribosomal/genetics

    KW - Female

    KW - Heterochromatin/genetics

    KW - Male

    KW - Phylogeny

    KW - Sex Chromosomes

    KW - Species Specificity

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    UR - http://www.mendeley.com/research/highly-conserved-z-molecularly-diverged-w-chromosomes-fish-genus-triportheus-characiformes-triporthe

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    DO - 10.1038/hdy.2016.83

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    EP - 283

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    SN - 0018-067X

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