Histone dynamics on the interleukin-2 gene in response to T-Cell activation

Xinxin Chen, Jun Wang, Donna Woltring, Steve Gerondakis, Frances Shannon

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Several models have been proposed for the mechanism of chromatin remodelling across the promoters of inducible genes in mammalian cells. The most commonly held model is one of cooccupation where histone proteins are modified by acetylation or phosphorylation and nucleosomes are remodelled, allowing the assembly of transcription factor complexes. Using chromatin immunoprecipitation, we observed an apparent decrease of histone acetylation and phosphorylation signals at the proximal promoter region of the inducible interleukin-2 and granulocyte-macrophage colony-stimulating factor genes in response to T-cell activation. We showed that this apparent decrease was due to a loss of histone H3 and H4 proteins corresponding to a decrease in nucleosome occupation of the promoter. This histone loss is reversible; it is dependent on the continual presence of appropriate activating signals and transcription factors and is not dependent on the acetylation status of the histone proteins. These data show for the first time that histone proteins are lost from a mammalian promoter upon activation of transcription and support a model of activation-dependent disassembly and reassembly of nucleosomes
Original languageEnglish
Pages (from-to)3209-3219
Number of pages11
JournalMolecular and Cellular Biology
Volume25
Issue number8
DOIs
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Histones
Interleukin-2
T-Lymphocytes
Nucleosomes
Genes
Acetylation
Proteins
Activating Transcription Factors
Phosphorylation
Chromatin Assembly and Disassembly
Chromatin Immunoprecipitation
Granulocyte-Macrophage Colony-Stimulating Factor
Occupations
Genetic Promoter Regions
Transcriptional Activation
Transcription Factors

Cite this

Chen, Xinxin ; Wang, Jun ; Woltring, Donna ; Gerondakis, Steve ; Shannon, Frances. / Histone dynamics on the interleukin-2 gene in response to T-Cell activation. In: Molecular and Cellular Biology. 2005 ; Vol. 25, No. 8. pp. 3209-3219.
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Histone dynamics on the interleukin-2 gene in response to T-Cell activation. / Chen, Xinxin; Wang, Jun; Woltring, Donna; Gerondakis, Steve; Shannon, Frances.

In: Molecular and Cellular Biology, Vol. 25, No. 8, 2005, p. 3209-3219.

Research output: Contribution to journalArticle

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T1 - Histone dynamics on the interleukin-2 gene in response to T-Cell activation

AU - Chen, Xinxin

AU - Wang, Jun

AU - Woltring, Donna

AU - Gerondakis, Steve

AU - Shannon, Frances

PY - 2005

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AB - Several models have been proposed for the mechanism of chromatin remodelling across the promoters of inducible genes in mammalian cells. The most commonly held model is one of cooccupation where histone proteins are modified by acetylation or phosphorylation and nucleosomes are remodelled, allowing the assembly of transcription factor complexes. Using chromatin immunoprecipitation, we observed an apparent decrease of histone acetylation and phosphorylation signals at the proximal promoter region of the inducible interleukin-2 and granulocyte-macrophage colony-stimulating factor genes in response to T-cell activation. We showed that this apparent decrease was due to a loss of histone H3 and H4 proteins corresponding to a decrease in nucleosome occupation of the promoter. This histone loss is reversible; it is dependent on the continual presence of appropriate activating signals and transcription factors and is not dependent on the acetylation status of the histone proteins. These data show for the first time that histone proteins are lost from a mammalian promoter upon activation of transcription and support a model of activation-dependent disassembly and reassembly of nucleosomes

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