HOW Is Required for Stem Cell Maintenance in the Drosophila Testis and for the Onset of Transit-Amplifying Divisions

Adrian C. Monk, Nicole A. Siddall, Talila Volk, Barbara Fraser, Leonie M. Quinn, Eileen A. McLaughlin, Gary R. Hime

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The mechanisms by which germline stem cells (GSCs) in the Drosophila testis undergo asymmetric division to regenerate a stem cell as well as a daughter (gonialblast) that will only undergo a further four mitotic divisions prior to entering premeiotic S phase and differentiating into a cyst of spermatocytes are not fully resolved. Here we demonstrate that the HOW RNA-binding protein is required for maintenance of CycB and therefore mitotic progression in GSCs and gonialblasts as well as determining the timing of the spermatogonial divisions. HOW is normally expressed in a complementary pattern to Bam in the germline and bam mRNA is bound by HOW in vivo. Ectopic expression of the HOW(L) isoform is associated with a delay in accumulation of Bam to the level required for differentiation, resulting in extra mitotic divisions. Spatiotemporal regulation of HOW expression is therefore required to specify the four spermatogonial transit-amplifying divisions.

Original languageEnglish
Pages (from-to)348-360
Number of pages13
JournalCell Stem Cell
Volume6
Issue number4
DOIs
Publication statusPublished - 2 Apr 2010
Externally publishedYes

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Drosophila
Testis
Stem Cells
Maintenance
Spermatocytes
RNA-Binding Proteins
S Phase
Cysts
Protein Isoforms
Messenger RNA
Ectopic Gene Expression

Cite this

Monk, A. C., Siddall, N. A., Volk, T., Fraser, B., Quinn, L. M., McLaughlin, E. A., & Hime, G. R. (2010). HOW Is Required for Stem Cell Maintenance in the Drosophila Testis and for the Onset of Transit-Amplifying Divisions. Cell Stem Cell, 6(4), 348-360. https://doi.org/10.1016/j.stem.2010.02.016
Monk, Adrian C. ; Siddall, Nicole A. ; Volk, Talila ; Fraser, Barbara ; Quinn, Leonie M. ; McLaughlin, Eileen A. ; Hime, Gary R. / HOW Is Required for Stem Cell Maintenance in the Drosophila Testis and for the Onset of Transit-Amplifying Divisions. In: Cell Stem Cell. 2010 ; Vol. 6, No. 4. pp. 348-360.
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Monk, AC, Siddall, NA, Volk, T, Fraser, B, Quinn, LM, McLaughlin, EA & Hime, GR 2010, 'HOW Is Required for Stem Cell Maintenance in the Drosophila Testis and for the Onset of Transit-Amplifying Divisions', Cell Stem Cell, vol. 6, no. 4, pp. 348-360. https://doi.org/10.1016/j.stem.2010.02.016

HOW Is Required for Stem Cell Maintenance in the Drosophila Testis and for the Onset of Transit-Amplifying Divisions. / Monk, Adrian C.; Siddall, Nicole A.; Volk, Talila; Fraser, Barbara; Quinn, Leonie M.; McLaughlin, Eileen A.; Hime, Gary R.

In: Cell Stem Cell, Vol. 6, No. 4, 02.04.2010, p. 348-360.

Research output: Contribution to journalArticle

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