Human catechol O-methyltransferase genetic variation

Gene resequencing and functional characterization of variant allozymes

A. J. Shield, B. A. Thomae, B. W. Eckloff, E. D. Wieben, R. M. Weinshilboum

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

Catechol O-methyltransferase (COMT) plays an Important role in the metabolism of catecholamlnes, catecholestrogens and catechol drugs. A common COMT G472A genetic polymorphism (Val108/158Met) that was identified previously is associated with decreased levels of enzyme activity and has been implicated as a possible risk factor for neuropsychiatric disease. We set out to 'resequence' the human COMT gene using DNA samples from 60 African-American and 60 Caucasian-American subjects. A total of 23 single nucleotlde polymorphisms (SNPs), including a novel nonsynonymous cSNP present only in DNA from African-American subjects, and one insertion/deletion were observed. The wild type (WT) and two variant allozymes, Thr52 and Met108, were transiently expressed in COS-1 and HEK293 cells. There was no significant change in level of COMT activity for the Thr52 variant allozyme, but there was a 40% decrease In the level of activity in cells transfected with the Met108 construct. Apparent Km values of the WT and variant allozymes for the two reaction cosubstrates differed slightly, but significantly, for 3,4-dihydroxybenzoic acid but not for S-adenosyl-L-methionine. The Met108 allozyme displayed a 70-90% decrease in immunoreactive protein when compared with WT, but there was no significant change in the level of immunoreactive protein for Thr52. A significant decrease in the level of immunoreactive protein was also observed in hepatic biopsy samples from patients homozygous for the allele encoding Met108. These observations represent steps toward an understanding of molecular genetic mechanisms responsible for variation in COMT level and/or properties, variation that may contribute to the pathophysiology of neuropsychiatric disease.

Original languageEnglish
Pages (from-to)151-160
Number of pages10
JournalMolecular Psychiatry
Volume9
Issue number2
DOIs
Publication statusPublished - 2004
Externally publishedYes

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Catechol O-Methyltransferase
Isoenzymes
Genes
African Americans
Catechol Estrogens
S-Adenosylmethionine
Proteins
HEK293 Cells
COS Cells
DNA
Genetic Polymorphisms
Molecular Biology
Alleles
Biopsy
Liver
Enzymes
Pharmaceutical Preparations

Cite this

Shield, A. J. ; Thomae, B. A. ; Eckloff, B. W. ; Wieben, E. D. ; Weinshilboum, R. M. / Human catechol O-methyltransferase genetic variation : Gene resequencing and functional characterization of variant allozymes. In: Molecular Psychiatry. 2004 ; Vol. 9, No. 2. pp. 151-160.
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abstract = "Catechol O-methyltransferase (COMT) plays an Important role in the metabolism of catecholamlnes, catecholestrogens and catechol drugs. A common COMT G472A genetic polymorphism (Val108/158Met) that was identified previously is associated with decreased levels of enzyme activity and has been implicated as a possible risk factor for neuropsychiatric disease. We set out to 'resequence' the human COMT gene using DNA samples from 60 African-American and 60 Caucasian-American subjects. A total of 23 single nucleotlde polymorphisms (SNPs), including a novel nonsynonymous cSNP present only in DNA from African-American subjects, and one insertion/deletion were observed. The wild type (WT) and two variant allozymes, Thr52 and Met108, were transiently expressed in COS-1 and HEK293 cells. There was no significant change in level of COMT activity for the Thr52 variant allozyme, but there was a 40{\%} decrease In the level of activity in cells transfected with the Met108 construct. Apparent Km values of the WT and variant allozymes for the two reaction cosubstrates differed slightly, but significantly, for 3,4-dihydroxybenzoic acid but not for S-adenosyl-L-methionine. The Met108 allozyme displayed a 70-90{\%} decrease in immunoreactive protein when compared with WT, but there was no significant change in the level of immunoreactive protein for Thr52. A significant decrease in the level of immunoreactive protein was also observed in hepatic biopsy samples from patients homozygous for the allele encoding Met108. These observations represent steps toward an understanding of molecular genetic mechanisms responsible for variation in COMT level and/or properties, variation that may contribute to the pathophysiology of neuropsychiatric disease.",
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Human catechol O-methyltransferase genetic variation : Gene resequencing and functional characterization of variant allozymes. / Shield, A. J.; Thomae, B. A.; Eckloff, B. W.; Wieben, E. D.; Weinshilboum, R. M.

In: Molecular Psychiatry, Vol. 9, No. 2, 2004, p. 151-160.

Research output: Contribution to journalArticle

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T1 - Human catechol O-methyltransferase genetic variation

T2 - Gene resequencing and functional characterization of variant allozymes

AU - Shield, A. J.

AU - Thomae, B. A.

AU - Eckloff, B. W.

AU - Wieben, E. D.

AU - Weinshilboum, R. M.

PY - 2004

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