Human MxA protein inhibits tick-borne Thogoto virus but not Dhori virus

Michael Frese, Georg Kochs, Ursula Meier-Dieter, Jürgen Siebler, Otto Haller

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Thogoto and Dhori viruses are tick-borne orthomyxoviruses infecting humans and livestock in Africa, Asia, and Europe. Here, we show that human MxA protein is an efficient inhibitor of Thogoto virus but is inactive against Dhori virus. When expressed in the cytoplasm of stably transfected cell lines, MxA protein interfered with the accumulation of Thogoto viral RNA and proteins. Likewise, MxA(R645), a mutant MxA protein known to be active against influenza virus but inactive against vesicular stomatitis virus, was equally efficient in blocking Thogoto virus growth. Hence, a common antiviral mechanism that is distinct from the antiviral action against vesicular stomatitis virus may operate against both influenza virus and Thogoto virus. When moved to the nucleus with the help of a foreign nuclear transport signal, MxA(R645) remained active against Thogoto virus, indicating that a nuclear step of virus replication was inhibited. In contrast, Dhori virus was not affected by wild-type or mutant MxA protein, indicating substantial differences between these two tick-transmitted orthomyxoviruses. Human MxB protein had no antiviral activity against either virus.

Original languageEnglish
Pages (from-to)3904-3909
Number of pages6
JournalJournal of Virology
Volume69
Issue number6
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Dhori virus
Thogoto virus
Thogotovirus
Ticks
ticks
Orthomyxoviridae
Vesiculovirus
proteins
Antiviral Agents
Vesicular Stomatitis
Mutant Proteins
Viruses
mutants
virus replication
Cell Nucleus Active Transport
human MX1 protein
Viral RNA
Livestock
Viral Proteins
cytoplasm

Cite this

Frese, M., Kochs, G., Meier-Dieter, U., Siebler, J., & Haller, O. (1995). Human MxA protein inhibits tick-borne Thogoto virus but not Dhori virus. Journal of Virology, 69(6), 3904-3909.
Frese, Michael ; Kochs, Georg ; Meier-Dieter, Ursula ; Siebler, Jürgen ; Haller, Otto. / Human MxA protein inhibits tick-borne Thogoto virus but not Dhori virus. In: Journal of Virology. 1995 ; Vol. 69, No. 6. pp. 3904-3909.
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Frese, M, Kochs, G, Meier-Dieter, U, Siebler, J & Haller, O 1995, 'Human MxA protein inhibits tick-borne Thogoto virus but not Dhori virus', Journal of Virology, vol. 69, no. 6, pp. 3904-3909.

Human MxA protein inhibits tick-borne Thogoto virus but not Dhori virus. / Frese, Michael; Kochs, Georg; Meier-Dieter, Ursula; Siebler, Jürgen; Haller, Otto.

In: Journal of Virology, Vol. 69, No. 6, 1995, p. 3904-3909.

Research output: Contribution to journalArticle

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AU - Frese, Michael

AU - Kochs, Georg

AU - Meier-Dieter, Ursula

AU - Siebler, Jürgen

AU - Haller, Otto

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Frese M, Kochs G, Meier-Dieter U, Siebler J, Haller O. Human MxA protein inhibits tick-borne Thogoto virus but not Dhori virus. Journal of Virology. 1995;69(6):3904-3909.