Identification multiplex assay of 19 terrestrial mammal species present in New Zealand

Ana Ramón-Laca, Adrian M T Linacre, Dianne GLEESON, Shanan S. Tobe

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    An identification assay has been developed that allows accurate detection of 19 of the most common terrestrial mammals present in New Zealand (cow, red deer, goat, dog, horse, hedgehog, cat, tammar wallaby, mouse, weasel, ferret, stoat, sheep, rabbit, Pacific rat, Norway rat, ship rat, pig, and brushtail possum). This technique utilizes species-specific primers that, combined in a multiplex PCR, target small fragments of the mitochondrial cytochrome b gene. Each species, except hedgehog, produces two distinctive species-specific fragments, making the assay self-confirmatory and enabling the identification of multiple species simultaneously in DNA mixtures. The multiplex assay detects as little as 100 copies of mitochondrial DNA,whichmakes it a very reliable tool for degraded and trace samples. Reliability, accuracy, reproducibility, and sensitivity tests to validate the technique were performed. The technique featured here enabled a prompt response in a predation specific event, but can also be useful for wildlife management and conservation, pest incursions detection, forensic, and industrial purposes in a very simple and cost-effective manner.
    Original languageEnglish
    Pages (from-to)3370-3377
    Number of pages8
    JournalElectrophoresis
    Volume34
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    Mammals
    New Zealand
    Rats
    Assays
    Hedgehogs
    Mustelidae
    Macropodidae
    Pest Control
    Ferrets
    Cytochromes b
    Deer
    Ships
    Multiplex Polymerase Chain Reaction
    Mitochondrial DNA
    Goats
    Horses
    Conservation
    Sheep
    Cats
    Swine

    Cite this

    Ramón-Laca, Ana ; Linacre, Adrian M T ; GLEESON, Dianne ; Tobe, Shanan S. / Identification multiplex assay of 19 terrestrial mammal species present in New Zealand. In: Electrophoresis. 2013 ; Vol. 34. pp. 3370-3377.
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    abstract = "An identification assay has been developed that allows accurate detection of 19 of the most common terrestrial mammals present in New Zealand (cow, red deer, goat, dog, horse, hedgehog, cat, tammar wallaby, mouse, weasel, ferret, stoat, sheep, rabbit, Pacific rat, Norway rat, ship rat, pig, and brushtail possum). This technique utilizes species-specific primers that, combined in a multiplex PCR, target small fragments of the mitochondrial cytochrome b gene. Each species, except hedgehog, produces two distinctive species-specific fragments, making the assay self-confirmatory and enabling the identification of multiple species simultaneously in DNA mixtures. The multiplex assay detects as little as 100 copies of mitochondrial DNA,whichmakes it a very reliable tool for degraded and trace samples. Reliability, accuracy, reproducibility, and sensitivity tests to validate the technique were performed. The technique featured here enabled a prompt response in a predation specific event, but can also be useful for wildlife management and conservation, pest incursions detection, forensic, and industrial purposes in a very simple and cost-effective manner.",
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    author = "Ana Ram{\'o}n-Laca and Linacre, {Adrian M T} and Dianne GLEESON and Tobe, {Shanan S.}",
    year = "2013",
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    Identification multiplex assay of 19 terrestrial mammal species present in New Zealand. / Ramón-Laca, Ana; Linacre, Adrian M T; GLEESON, Dianne; Tobe, Shanan S.

    In: Electrophoresis, Vol. 34, 2013, p. 3370-3377.

    Research output: Contribution to journalArticle

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    AU - Linacre, Adrian M T

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    AB - An identification assay has been developed that allows accurate detection of 19 of the most common terrestrial mammals present in New Zealand (cow, red deer, goat, dog, horse, hedgehog, cat, tammar wallaby, mouse, weasel, ferret, stoat, sheep, rabbit, Pacific rat, Norway rat, ship rat, pig, and brushtail possum). This technique utilizes species-specific primers that, combined in a multiplex PCR, target small fragments of the mitochondrial cytochrome b gene. Each species, except hedgehog, produces two distinctive species-specific fragments, making the assay self-confirmatory and enabling the identification of multiple species simultaneously in DNA mixtures. The multiplex assay detects as little as 100 copies of mitochondrial DNA,whichmakes it a very reliable tool for degraded and trace samples. Reliability, accuracy, reproducibility, and sensitivity tests to validate the technique were performed. The technique featured here enabled a prompt response in a predation specific event, but can also be useful for wildlife management and conservation, pest incursions detection, forensic, and industrial purposes in a very simple and cost-effective manner.

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    KW - Pest detection

    KW - Species identification.

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